Genetic homogeneity of vegetatively propagated Clinacanthus nutans (Acanthaceae)
Clinacanthus nutans is a medicinal Asian plant often propagated by stem cuttings but little is known about the genetic relationships between existing accessions and the extent of homogeneity. In this study, we examined the genetic homogeneity in 12 C. nutans samples from Malaysia, Thailand and Vietn...
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| Format: | Article |
| Language: | English English |
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Academic Journals
2014
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| Online Access: | https://eprints.ums.edu.my/id/eprint/20253/1/Genetic%20homogeneity%20of%20vegetatively%20propagated%20Clinacanthus%20nutans.pdf https://eprints.ums.edu.my/id/eprint/20253/7/Genetic%20homogeneity%20of%20vegetatively%20propagated%20Clinacanthus%20nutans%20%28Acanthaceae%29.pdf |
| _version_ | 1825713488609148928 |
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| author | Fong, Siat Yee Piva, Terrence Urban, Sylvia Tien Huynh |
| author_facet | Fong, Siat Yee Piva, Terrence Urban, Sylvia Tien Huynh |
| author_sort | Fong, Siat Yee |
| collection | UMS |
| description | Clinacanthus nutans is a medicinal Asian plant often propagated by stem cuttings but little is known about the genetic relationships between existing accessions and the extent of homogeneity. In this study, we examined the genetic homogeneity in 12 C. nutans samples from Malaysia, Thailand and Vietnam reproduced by vegetative propagation from different regions between and within countries, and compared it to sexually propagated Andrographis paniculata (same family), related Clinacanthus siamensis (same family) and an out-group (different family) using restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) and microsatellite markers. There was a high genetic similarity between C. nutans accessions from all countries, with identical genetic profiles even though they were geographically distant. C. nutans clustered closely with C. siamensis and was distant from A. paniculata and the out-group. Genetic similarity for C. nutans was almost double that of A. paniculata, but the combined clustering analysis revealed higher diversity in C. nutans. These results provide fundamental knowledge in future planting decisions and options, and also facilitate further germplasm conservation of C. nutans and other vegetatively propagated medicinal species. |
| first_indexed | 2024-03-06T02:57:22Z |
| format | Article |
| id | ums.eprints-20253 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T02:57:22Z |
| publishDate | 2014 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | ums.eprints-202532021-01-11T08:44:55Z https://eprints.ums.edu.my/id/eprint/20253/ Genetic homogeneity of vegetatively propagated Clinacanthus nutans (Acanthaceae) Fong, Siat Yee Piva, Terrence Urban, Sylvia Tien Huynh RS Pharmacy and materia medica Clinacanthus nutans is a medicinal Asian plant often propagated by stem cuttings but little is known about the genetic relationships between existing accessions and the extent of homogeneity. In this study, we examined the genetic homogeneity in 12 C. nutans samples from Malaysia, Thailand and Vietnam reproduced by vegetative propagation from different regions between and within countries, and compared it to sexually propagated Andrographis paniculata (same family), related Clinacanthus siamensis (same family) and an out-group (different family) using restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) and microsatellite markers. There was a high genetic similarity between C. nutans accessions from all countries, with identical genetic profiles even though they were geographically distant. C. nutans clustered closely with C. siamensis and was distant from A. paniculata and the out-group. Genetic similarity for C. nutans was almost double that of A. paniculata, but the combined clustering analysis revealed higher diversity in C. nutans. These results provide fundamental knowledge in future planting decisions and options, and also facilitate further germplasm conservation of C. nutans and other vegetatively propagated medicinal species. Academic Journals 2014-07 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/20253/1/Genetic%20homogeneity%20of%20vegetatively%20propagated%20Clinacanthus%20nutans.pdf text en https://eprints.ums.edu.my/id/eprint/20253/7/Genetic%20homogeneity%20of%20vegetatively%20propagated%20Clinacanthus%20nutans%20%28Acanthaceae%29.pdf Fong, Siat Yee and Piva, Terrence and Urban, Sylvia and Tien Huynh (2014) Genetic homogeneity of vegetatively propagated Clinacanthus nutans (Acanthaceae). Journal of Medicinal Plants Research, 8 (25). pp. 903-914. ISSN 1996-0875 https://doi.org/10.5897/JMPR2013.5328 |
| spellingShingle | RS Pharmacy and materia medica Fong, Siat Yee Piva, Terrence Urban, Sylvia Tien Huynh Genetic homogeneity of vegetatively propagated Clinacanthus nutans (Acanthaceae) |
| title | Genetic homogeneity of vegetatively propagated Clinacanthus nutans (Acanthaceae) |
| title_full | Genetic homogeneity of vegetatively propagated Clinacanthus nutans (Acanthaceae) |
| title_fullStr | Genetic homogeneity of vegetatively propagated Clinacanthus nutans (Acanthaceae) |
| title_full_unstemmed | Genetic homogeneity of vegetatively propagated Clinacanthus nutans (Acanthaceae) |
| title_short | Genetic homogeneity of vegetatively propagated Clinacanthus nutans (Acanthaceae) |
| title_sort | genetic homogeneity of vegetatively propagated clinacanthus nutans acanthaceae |
| topic | RS Pharmacy and materia medica |
| url | https://eprints.ums.edu.my/id/eprint/20253/1/Genetic%20homogeneity%20of%20vegetatively%20propagated%20Clinacanthus%20nutans.pdf https://eprints.ums.edu.my/id/eprint/20253/7/Genetic%20homogeneity%20of%20vegetatively%20propagated%20Clinacanthus%20nutans%20%28Acanthaceae%29.pdf |
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