| Summary: | This report described the growth characteristics and production of physalins from Physalis
minima hairy roots in shake fl ask culture. The presence of lateral branches in the inoculum had a
negligible effect on the fi nal root biomass dry weight (DW), specifi c growth rate (μ), doubling time (td)
and production of physalins. However, excising the primary and lateral root tips reduced μ and the total
root length but not the fi nal biomass. Mature root tissues were observed to accumulate more physalin
B and F (1.55 and 3.74 mg.g-1 DW, respectively) compared to the root tips (0.65 and 1.47 mg.g-1 DW,
respectively). Increasing the number of root tips from 2 to 12 and the medium volume signifi cantly
reduced μ and extended td. Decreasing the medium volume with a small number of inocula reduced td,
improved the biomass and production of physalins and μ. Using a 100 mL fl ask, four root tips cultured in
25 mL medium provided the optimum conditions for biomass (0.24 g DW) and production of physalins
(1.68–3.5 mg.g-1 DW).
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