Antibacterials and phytochemicals investigations of Chromolaenaodorata (L.F.) King and Robinson (asteraceae) from Sabah, Malaysia
Antimicrobial properties and phytochemical constituents in leaf extract of Chromolaenaodorata was evaluated in this study. C. odorataleaves were subjected to liquid-liquid extraction by using methanol, hexane, ethyl acetate, chloroform, buthanol and water. All extract partitions were tested for anti...
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| Format: | Article |
| Language: | English |
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2013
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| Online Access: | https://eprints.ums.edu.my/id/eprint/21500/1/Antibacterials%20and%20phytochemicals%20investigations%20of%20Chromolaenaodorata.pdf |
| _version_ | 1825713568620740608 |
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| author | Jualang Azlan Gansau |
| author_facet | Jualang Azlan Gansau |
| author_sort | Jualang Azlan Gansau |
| collection | UMS |
| description | Antimicrobial properties and phytochemical constituents in leaf extract of Chromolaenaodorata was evaluated in this study. C. odorataleaves were subjected to liquid-liquid extraction by using methanol, hexane, ethyl acetate, chloroform, buthanol and water. All extract partitions were tested for antibacterial activity against five Gram positive and Gram negative bacteria by using disc diffusion method. Crude methanolic extract (CME), ethyl acetate extract (EAE) and chloroform extract (CE) showed good antibacterial properties against the tested bacterial strains. However, only the CE was further separated using silica column chromatography. About 10 semi purified fractions was obtained and fraction 2 (F2) showed consistent inhibitory zones against all bacterial tested. Phytochemical investigations on the extract partitions and fractions showed the presence of alkaloids, flavonoids, tannins, polyphenols, saponins and triterpenoids. Fraction F2 was subjected to GC-MS analysis to characterised the bioactive compounds. The GC-MS spectral data has identified 10 major compounds which are hexachloro-ethane, n-nonylaldehyde, methyl-4-oxooctanoate, longiverbenone, 2-butenal,2-methyl-4-(2,6,6-trimethyl-1-cyclohexen-1-yl), neophytadiene, phytol, dihydro-neoclovene, 2,6-ditert-butylquinone and aromadendrene |
| first_indexed | 2024-03-06T02:58:36Z |
| format | Article |
| id | ums.eprints-21500 |
| institution | Universiti Malaysia Sabah |
| language | English |
| last_indexed | 2024-03-06T02:58:36Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ums.eprints-215002019-03-05T04:38:21Z https://eprints.ums.edu.my/id/eprint/21500/ Antibacterials and phytochemicals investigations of Chromolaenaodorata (L.F.) King and Robinson (asteraceae) from Sabah, Malaysia Jualang Azlan Gansau QK Botany Antimicrobial properties and phytochemical constituents in leaf extract of Chromolaenaodorata was evaluated in this study. C. odorataleaves were subjected to liquid-liquid extraction by using methanol, hexane, ethyl acetate, chloroform, buthanol and water. All extract partitions were tested for antibacterial activity against five Gram positive and Gram negative bacteria by using disc diffusion method. Crude methanolic extract (CME), ethyl acetate extract (EAE) and chloroform extract (CE) showed good antibacterial properties against the tested bacterial strains. However, only the CE was further separated using silica column chromatography. About 10 semi purified fractions was obtained and fraction 2 (F2) showed consistent inhibitory zones against all bacterial tested. Phytochemical investigations on the extract partitions and fractions showed the presence of alkaloids, flavonoids, tannins, polyphenols, saponins and triterpenoids. Fraction F2 was subjected to GC-MS analysis to characterised the bioactive compounds. The GC-MS spectral data has identified 10 major compounds which are hexachloro-ethane, n-nonylaldehyde, methyl-4-oxooctanoate, longiverbenone, 2-butenal,2-methyl-4-(2,6,6-trimethyl-1-cyclohexen-1-yl), neophytadiene, phytol, dihydro-neoclovene, 2,6-ditert-butylquinone and aromadendrene 2013 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/21500/1/Antibacterials%20and%20phytochemicals%20investigations%20of%20Chromolaenaodorata.pdf Jualang Azlan Gansau (2013) Antibacterials and phytochemicals investigations of Chromolaenaodorata (L.F.) King and Robinson (asteraceae) from Sabah, Malaysia. Journal Of Environmental Microbiology And Toxicology, 1 (1). pp. 18-24. |
| spellingShingle | QK Botany Jualang Azlan Gansau Antibacterials and phytochemicals investigations of Chromolaenaodorata (L.F.) King and Robinson (asteraceae) from Sabah, Malaysia |
| title | Antibacterials and phytochemicals investigations of Chromolaenaodorata (L.F.) King and Robinson (asteraceae) from Sabah, Malaysia |
| title_full | Antibacterials and phytochemicals investigations of Chromolaenaodorata (L.F.) King and Robinson (asteraceae) from Sabah, Malaysia |
| title_fullStr | Antibacterials and phytochemicals investigations of Chromolaenaodorata (L.F.) King and Robinson (asteraceae) from Sabah, Malaysia |
| title_full_unstemmed | Antibacterials and phytochemicals investigations of Chromolaenaodorata (L.F.) King and Robinson (asteraceae) from Sabah, Malaysia |
| title_short | Antibacterials and phytochemicals investigations of Chromolaenaodorata (L.F.) King and Robinson (asteraceae) from Sabah, Malaysia |
| title_sort | antibacterials and phytochemicals investigations of chromolaenaodorata l f king and robinson asteraceae from sabah malaysia |
| topic | QK Botany |
| url | https://eprints.ums.edu.my/id/eprint/21500/1/Antibacterials%20and%20phytochemicals%20investigations%20of%20Chromolaenaodorata.pdf |
| work_keys_str_mv | AT jualangazlangansau antibacterialsandphytochemicalsinvestigationsofchromolaenaodoratalfkingandrobinsonasteraceaefromsabahmalaysia |