Induction and Microscopic Characterization of Globular Callus from Stem Explant of Labisia pumila var. alata

Labisia pumila (BI.) F. Vill (Myrsinaceae) also known as Kacip Fatimah is a medicinal plant with an enormous therapeutics value and used widely for herbal industries in Malaysia. This study reports the induction of globular calli from stem explants of L. pumila var. alata....

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主要な著者: Jualang Azlan Gansau, Rozalia Mohd Geoffery, Suraya, Devina David, Roslina Jawan, Hartinie Marbawi
フォーマット: 論文
言語:English
English
出版事項: 2015
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オンライン・アクセス:https://eprints.ums.edu.my/id/eprint/21813/1/Induction%20and%20microscopic%20characterization%20of%20globular%20callus%20from%20stem%20explant%20of%20Labisia%20pumila%20var.%20alata..pdf
https://eprints.ums.edu.my/id/eprint/21813/7/Induction%20and%20Microscopic%20Characterization%20of%20Globular%20Callus%20from%20Stem%20Explant%20of%20Labisia%20pumila%20var.%20alata..pdf
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author Jualang Azlan Gansau
Rozalia Mohd Geoffery
Suraya
Devina David
Roslina Jawan
Hartinie Marbawi
author_facet Jualang Azlan Gansau
Rozalia Mohd Geoffery
Suraya
Devina David
Roslina Jawan
Hartinie Marbawi
author_sort Jualang Azlan Gansau
collection UMS
description Labisia pumila (BI.) F. Vill (Myrsinaceae) also known as Kacip Fatimah is a medicinal plant with an enormous therapeutics value and used widely for herbal industries in Malaysia. This study reports the induction of globular calli from stem explants of L. pumila var. alata. The embryogenic characteristics of the globular calli were further confirmed by the morphological and histological analyses using the light microscope of sectioned material and scanning electron microscope. Globular callus was induced from stem explants that were pre-treated/un-pretreated with thidiazuron (TDZ) on Murashige and Skoog (MS) medium supplemented with 20 µ M 2,4-dichlorophenoxyacetic acid (2,4-D) and various amino acids (glutamine, arginine, proline and leucine) at the concentrations of 0.05, 0.1, 0.25 and 0.5 mM. After six weeks of incubation, explants pre-treated with TDZ induced 100% of globular callus while only friable calli were observed on un-pretreated explants. The addition of amino acids at concentrations between 0.05-0.25 mM enhanced the induction of globular callus. Off all, 0.25 mM leucine provided the highest callus score (5.93±0.12) which produced 31 to 40 globular callus per explant. Double staining test shows the positive reaction to aceto-carmine red (0.5%) in the globular callus indicating the existence of embryogenic nature. Analysis under SEM and histological evaluation has confirmed the embryogenic characteristics with the appearance of isodiametric clump cells containing prominent nuclei. The globular callus is further regenerated to shoot clump, thus, it is suggested that the use of TDZ-pretreatment method with addition of 20 µM 2,4-D and 0.25mM leucine successfully induced embryogenic callus from stem explant of L. pumila var. alata. Copyright © 2015 Penerbit Akademia Baru - All rights reserved
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spelling ums.eprints-218132021-04-30T07:46:31Z https://eprints.ums.edu.my/id/eprint/21813/ Induction and Microscopic Characterization of Globular Callus from Stem Explant of Labisia pumila var. alata Jualang Azlan Gansau Rozalia Mohd Geoffery Suraya Devina David Roslina Jawan Hartinie Marbawi QK Botany Labisia pumila (BI.) F. Vill (Myrsinaceae) also known as Kacip Fatimah is a medicinal plant with an enormous therapeutics value and used widely for herbal industries in Malaysia. This study reports the induction of globular calli from stem explants of L. pumila var. alata. The embryogenic characteristics of the globular calli were further confirmed by the morphological and histological analyses using the light microscope of sectioned material and scanning electron microscope. Globular callus was induced from stem explants that were pre-treated/un-pretreated with thidiazuron (TDZ) on Murashige and Skoog (MS) medium supplemented with 20 µ M 2,4-dichlorophenoxyacetic acid (2,4-D) and various amino acids (glutamine, arginine, proline and leucine) at the concentrations of 0.05, 0.1, 0.25 and 0.5 mM. After six weeks of incubation, explants pre-treated with TDZ induced 100% of globular callus while only friable calli were observed on un-pretreated explants. The addition of amino acids at concentrations between 0.05-0.25 mM enhanced the induction of globular callus. Off all, 0.25 mM leucine provided the highest callus score (5.93±0.12) which produced 31 to 40 globular callus per explant. Double staining test shows the positive reaction to aceto-carmine red (0.5%) in the globular callus indicating the existence of embryogenic nature. Analysis under SEM and histological evaluation has confirmed the embryogenic characteristics with the appearance of isodiametric clump cells containing prominent nuclei. The globular callus is further regenerated to shoot clump, thus, it is suggested that the use of TDZ-pretreatment method with addition of 20 µM 2,4-D and 0.25mM leucine successfully induced embryogenic callus from stem explant of L. pumila var. alata. Copyright © 2015 Penerbit Akademia Baru - All rights reserved 2015 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/21813/1/Induction%20and%20microscopic%20characterization%20of%20globular%20callus%20from%20stem%20explant%20of%20Labisia%20pumila%20var.%20alata..pdf text en https://eprints.ums.edu.my/id/eprint/21813/7/Induction%20and%20Microscopic%20Characterization%20of%20Globular%20Callus%20from%20Stem%20Explant%20of%20Labisia%20pumila%20var.%20alata..pdf Jualang Azlan Gansau and Rozalia Mohd Geoffery and Suraya and Devina David and Roslina Jawan and Hartinie Marbawi (2015) Induction and Microscopic Characterization of Globular Callus from Stem Explant of Labisia pumila var. alata. Journal of Advanced Research in Materials Sciences, 9 (1). pp. 1-12.
spellingShingle QK Botany
Jualang Azlan Gansau
Rozalia Mohd Geoffery
Suraya
Devina David
Roslina Jawan
Hartinie Marbawi
Induction and Microscopic Characterization of Globular Callus from Stem Explant of Labisia pumila var. alata
title Induction and Microscopic Characterization of Globular Callus from Stem Explant of Labisia pumila var. alata
title_full Induction and Microscopic Characterization of Globular Callus from Stem Explant of Labisia pumila var. alata
title_fullStr Induction and Microscopic Characterization of Globular Callus from Stem Explant of Labisia pumila var. alata
title_full_unstemmed Induction and Microscopic Characterization of Globular Callus from Stem Explant of Labisia pumila var. alata
title_short Induction and Microscopic Characterization of Globular Callus from Stem Explant of Labisia pumila var. alata
title_sort induction and microscopic characterization of globular callus from stem explant of labisia pumila var alata
topic QK Botany
url https://eprints.ums.edu.my/id/eprint/21813/1/Induction%20and%20microscopic%20characterization%20of%20globular%20callus%20from%20stem%20explant%20of%20Labisia%20pumila%20var.%20alata..pdf
https://eprints.ums.edu.my/id/eprint/21813/7/Induction%20and%20Microscopic%20Characterization%20of%20Globular%20Callus%20from%20Stem%20Explant%20of%20Labisia%20pumila%20var.%20alata..pdf
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