Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques
The distribution of hydrocarbon-utilizing bacterial may vary between surface and subsurface of the seawater. One of the identified contributors is the Total Petroleum Hydrocarbon. The isolation and characterization of bacteria using Direct and Enrichment techniques helps in identifying dominant bact...
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| Format: | Article |
| Language: | English English |
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78(1) · April 2015
2015
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| Online Access: | https://eprints.ums.edu.my/id/eprint/21978/1/Isolation%20and%20Characterization%20of%20Surface%20and%20Subsurface%20Bacteria%20in%20Seawater%20of%20Mantanani%20Island.pdf https://eprints.ums.edu.my/id/eprint/21978/7/Isolation%20and%20Characterization%20of%20Surface%20and%20Subsurface%20Bacteria%20in%20Seawater%20of%20Mantanani%20Island%2C%20Kota%20Belud%2C%20Sabah%20by%20Direct%20and%20Enrichment%20Techniques..pdf |
| _version_ | 1796910094813757440 |
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| author | Benard, L D Tuah, P.M Suadin, E G Jamian, N |
| author_facet | Benard, L D Tuah, P.M Suadin, E G Jamian, N |
| author_sort | Benard, L D |
| collection | UMS |
| description | The distribution of hydrocarbon-utilizing bacterial may vary between surface and subsurface of the seawater. One of the identified contributors is the Total Petroleum Hydrocarbon. The isolation and characterization of bacteria using Direct and Enrichment techniques helps in identifying dominant bacterial populations in seawater of Mantanani Island, Kota Belud, Sabah, potential of further investigation as hydrocarbon degrader. Crude oil (5% v/v) was added as the carbon source for bacteria in Enrichment technique. For surface seawater, the highest population of bacteria identified for both Direct and Enrichment technique were 2.60 × 107 CFU/mL and 3.84 × 106 CFU/mL respectively. Meanwhile, for subsurface seawater, the highest population of bacteria identified for both Direct and Enrichment technique were 5.21 × 106 CFU/mL and 8.99 × 107 CFU/mL respectively. Dominant species in surface seawater were characterized as Marinobacter hydrocarbonoclasticus-RMSF-C1 and RMSF-C2 and Alcanivorax borkumensis-RMSF-C3, RMSF-C4 and RMSF-C5. As for subsurface seawater, dominant species were characterized as Pseudomonas luteola-SSBR-W1, Burkholderia cepacia-SSBR-C1, Rhizobium radiobacter- SSBR-C3 and Leuconostoc-cremois -SSBR-C4. |
| first_indexed | 2024-03-06T02:59:20Z |
| format | Article |
| id | ums.eprints-21978 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T02:59:20Z |
| publishDate | 2015 |
| publisher | 78(1) · April 2015 |
| record_format | dspace |
| spelling | ums.eprints-219782021-04-30T07:53:42Z https://eprints.ums.edu.my/id/eprint/21978/ Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques Benard, L D Tuah, P.M Suadin, E G Jamian, N The distribution of hydrocarbon-utilizing bacterial may vary between surface and subsurface of the seawater. One of the identified contributors is the Total Petroleum Hydrocarbon. The isolation and characterization of bacteria using Direct and Enrichment techniques helps in identifying dominant bacterial populations in seawater of Mantanani Island, Kota Belud, Sabah, potential of further investigation as hydrocarbon degrader. Crude oil (5% v/v) was added as the carbon source for bacteria in Enrichment technique. For surface seawater, the highest population of bacteria identified for both Direct and Enrichment technique were 2.60 × 107 CFU/mL and 3.84 × 106 CFU/mL respectively. Meanwhile, for subsurface seawater, the highest population of bacteria identified for both Direct and Enrichment technique were 5.21 × 106 CFU/mL and 8.99 × 107 CFU/mL respectively. Dominant species in surface seawater were characterized as Marinobacter hydrocarbonoclasticus-RMSF-C1 and RMSF-C2 and Alcanivorax borkumensis-RMSF-C3, RMSF-C4 and RMSF-C5. As for subsurface seawater, dominant species were characterized as Pseudomonas luteola-SSBR-W1, Burkholderia cepacia-SSBR-C1, Rhizobium radiobacter- SSBR-C3 and Leuconostoc-cremois -SSBR-C4. 78(1) · April 2015 2015 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/21978/1/Isolation%20and%20Characterization%20of%20Surface%20and%20Subsurface%20Bacteria%20in%20Seawater%20of%20Mantanani%20Island.pdf text en https://eprints.ums.edu.my/id/eprint/21978/7/Isolation%20and%20Characterization%20of%20Surface%20and%20Subsurface%20Bacteria%20in%20Seawater%20of%20Mantanani%20Island%2C%20Kota%20Belud%2C%20Sabah%20by%20Direct%20and%20Enrichment%20Techniques..pdf Benard, L D and Tuah, P.M and Suadin, E G and Jamian, N (2015) Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques. Materials Science & Engineering, 78 (1). DOI: 10.1088/1757-899X/78/1/012033 |
| spellingShingle | Benard, L D Tuah, P.M Suadin, E G Jamian, N Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques |
| title | Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques |
| title_full | Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques |
| title_fullStr | Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques |
| title_full_unstemmed | Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques |
| title_short | Isolation and Characterization of Surface and Subsurface Bacteria in Seawater of Mantanani Island, Kota Belud, Sabah by Direct and Enrichment Techniques |
| title_sort | isolation and characterization of surface and subsurface bacteria in seawater of mantanani island kota belud sabah by direct and enrichment techniques |
| url | https://eprints.ums.edu.my/id/eprint/21978/1/Isolation%20and%20Characterization%20of%20Surface%20and%20Subsurface%20Bacteria%20in%20Seawater%20of%20Mantanani%20Island.pdf https://eprints.ums.edu.my/id/eprint/21978/7/Isolation%20and%20Characterization%20of%20Surface%20and%20Subsurface%20Bacteria%20in%20Seawater%20of%20Mantanani%20Island%2C%20Kota%20Belud%2C%20Sabah%20by%20Direct%20and%20Enrichment%20Techniques..pdf |
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