Glutathione, Antioxidant Enzymes and Oxidative Stress in Acute and Subacute Exposure of Diazinon-Mediated Renal Oxidative Injury in Rats
This study aimed at investigating the possible nephrotoxic effects of the diazinon induced oxidative stress in rats following the acute and subacute administration. Oxidative stress markers in renal tissues, as well as serum biochemical parameters, were evaluated using colorimetric spectrophotometri...
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| Format: | Article |
| Language: | English English |
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Science Signpost Publishing
2019
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| Online Access: | https://eprints.ums.edu.my/id/eprint/25787/1/Glutathione%2C%20Antioxidant%20Enzymes%20and%20Oxidative%20Stress%20in%20Acute%20and%20Subacute%20Exposure%20of%20Diazinon-Mediated%20Renal%20Oxidative%20Injury%20in%20Rats.pdf https://eprints.ums.edu.my/id/eprint/25787/2/Glutathione%2C%20Antioxidant%20Enzymes%20and%20Oxidative%20Stress%20in%20Acute%20and%20Subacute%20Exposure%20of%20Diazinon-Mediated%20Renal%20Oxidative%20Injury%20in%20Rats1.pdf |
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| author | Muhammad Dawood Shah Urban J.A.D’Souza Mohammad Iqbal |
| author_facet | Muhammad Dawood Shah Urban J.A.D’Souza Mohammad Iqbal |
| author_sort | Muhammad Dawood Shah |
| collection | UMS |
| description | This study aimed at investigating the possible nephrotoxic effects of the diazinon induced oxidative stress in rats following the acute and subacute administration. Oxidative stress markers in renal tissues, as well as serum biochemical parameters, were evaluated using colorimetric spectrophotometric techniques. Our data showed that diazinon administration to rats induced oxidative stress in kidney, as evidenced by increasing of renal lipid peroxidation level which was accompanied by decreased activities of antioxidant enzymes and depletion in the level of GSH (p<0.05) compared to saline-treated control. The activities of renal ƴ-glutamyl transpeptidase and quinone reductase were increased (p<0.05) compared to saline-treated control. In addition, diazinon treatment augments renal injuries as evident by the increase in serum creatinine and blood urea nitrogen (p<0.05). Histopathological analysis of renal tissues was in concurrence with the biochemical studies. Overall results suggest that oxidative stress-induced lipid peroxidation and alteration in activities of glutathione and antioxidant enzymes play roles in diazinon- mediated renal injury and toxicity in rats. |
| first_indexed | 2024-03-06T03:04:13Z |
| format | Article |
| id | ums.eprints-25787 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:04:13Z |
| publishDate | 2019 |
| publisher | Science Signpost Publishing |
| record_format | dspace |
| spelling | ums.eprints-257872020-08-12T02:55:02Z https://eprints.ums.edu.my/id/eprint/25787/ Glutathione, Antioxidant Enzymes and Oxidative Stress in Acute and Subacute Exposure of Diazinon-Mediated Renal Oxidative Injury in Rats Muhammad Dawood Shah Urban J.A.D’Souza Mohammad Iqbal Q Science (General) This study aimed at investigating the possible nephrotoxic effects of the diazinon induced oxidative stress in rats following the acute and subacute administration. Oxidative stress markers in renal tissues, as well as serum biochemical parameters, were evaluated using colorimetric spectrophotometric techniques. Our data showed that diazinon administration to rats induced oxidative stress in kidney, as evidenced by increasing of renal lipid peroxidation level which was accompanied by decreased activities of antioxidant enzymes and depletion in the level of GSH (p<0.05) compared to saline-treated control. The activities of renal ƴ-glutamyl transpeptidase and quinone reductase were increased (p<0.05) compared to saline-treated control. In addition, diazinon treatment augments renal injuries as evident by the increase in serum creatinine and blood urea nitrogen (p<0.05). Histopathological analysis of renal tissues was in concurrence with the biochemical studies. Overall results suggest that oxidative stress-induced lipid peroxidation and alteration in activities of glutathione and antioxidant enzymes play roles in diazinon- mediated renal injury and toxicity in rats. Science Signpost Publishing 2019 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/25787/1/Glutathione%2C%20Antioxidant%20Enzymes%20and%20Oxidative%20Stress%20in%20Acute%20and%20Subacute%20Exposure%20of%20Diazinon-Mediated%20Renal%20Oxidative%20Injury%20in%20Rats.pdf text en https://eprints.ums.edu.my/id/eprint/25787/2/Glutathione%2C%20Antioxidant%20Enzymes%20and%20Oxidative%20Stress%20in%20Acute%20and%20Subacute%20Exposure%20of%20Diazinon-Mediated%20Renal%20Oxidative%20Injury%20in%20Rats1.pdf Muhammad Dawood Shah and Urban J.A.D’Souza and Mohammad Iqbal (2019) Glutathione, Antioxidant Enzymes and Oxidative Stress in Acute and Subacute Exposure of Diazinon-Mediated Renal Oxidative Injury in Rats. Biological and Chemical Research, 6. pp. 135-149. ISSN 2312-0088 |
| spellingShingle | Q Science (General) Muhammad Dawood Shah Urban J.A.D’Souza Mohammad Iqbal Glutathione, Antioxidant Enzymes and Oxidative Stress in Acute and Subacute Exposure of Diazinon-Mediated Renal Oxidative Injury in Rats |
| title | Glutathione, Antioxidant Enzymes and Oxidative Stress in Acute and Subacute Exposure of Diazinon-Mediated Renal Oxidative Injury in Rats |
| title_full | Glutathione, Antioxidant Enzymes and Oxidative Stress in Acute and Subacute Exposure of Diazinon-Mediated Renal Oxidative Injury in Rats |
| title_fullStr | Glutathione, Antioxidant Enzymes and Oxidative Stress in Acute and Subacute Exposure of Diazinon-Mediated Renal Oxidative Injury in Rats |
| title_full_unstemmed | Glutathione, Antioxidant Enzymes and Oxidative Stress in Acute and Subacute Exposure of Diazinon-Mediated Renal Oxidative Injury in Rats |
| title_short | Glutathione, Antioxidant Enzymes and Oxidative Stress in Acute and Subacute Exposure of Diazinon-Mediated Renal Oxidative Injury in Rats |
| title_sort | glutathione antioxidant enzymes and oxidative stress in acute and subacute exposure of diazinon mediated renal oxidative injury in rats |
| topic | Q Science (General) |
| url | https://eprints.ums.edu.my/id/eprint/25787/1/Glutathione%2C%20Antioxidant%20Enzymes%20and%20Oxidative%20Stress%20in%20Acute%20and%20Subacute%20Exposure%20of%20Diazinon-Mediated%20Renal%20Oxidative%20Injury%20in%20Rats.pdf https://eprints.ums.edu.my/id/eprint/25787/2/Glutathione%2C%20Antioxidant%20Enzymes%20and%20Oxidative%20Stress%20in%20Acute%20and%20Subacute%20Exposure%20of%20Diazinon-Mediated%20Renal%20Oxidative%20Injury%20in%20Rats1.pdf |
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