Structural Insights into the Enzymatic Activity of Cysteine Protease Bromelain of MD2 Pineapple
Background and Objective: The MD2 pineapple contains 14 various sizes of bromelain (MD2-bromelains) ranging from 19-200 kDa which are suspected to be structurally and enzymatically varied. This study aims to compare the enzymatic activity and structural features of small and medium-sizes of MD2-brom...
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| Format: | Article |
| Language: | English English |
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2020
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| Online Access: | https://eprints.ums.edu.my/id/eprint/25972/1/Structural%20Insights%20into%20the%20Enzymatic%20Activity%20of%20Cysteine%20Protease%20Bromelain%20of%20MD2%20Pineapple.pdf https://eprints.ums.edu.my/id/eprint/25972/2/Structural%20Insights%20into%20the%20Enzymatic%20Activity%20of%20Cysteine%20Protease%20Bromelain%20of%20MD2%20Pineapple1.pdf |
| _version_ | 1796910444571525120 |
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| author | Rafida Razali Vijay Kumar Subbiah Cahyo Budiman |
| author_facet | Rafida Razali Vijay Kumar Subbiah Cahyo Budiman |
| author_sort | Rafida Razali |
| collection | UMS |
| description | Background and Objective: The MD2 pineapple contains 14 various sizes of bromelain (MD2-bromelains) ranging from 19-200 kDa which are suspected to be structurally and enzymatically varied. This study aims to compare the enzymatic activity and structural features of small and medium-sizes of MD2-bromelains, designated as MD2-SBro (19 kDa) and MD2-MBro (38 kDa), respectively. Materials and Methods: Purified recombinant MD2-SBro and MD2-MBro obtained were used in this study. The enzymatic activity of both MD2-bromelain was determined using a plate agar system with casein as a substrate. Three-dimensional (3D) structures of both MD2-bromelains were constructed under SWISS-MODEL server-based structural homology modeling and verified stereo-chemically. Results: The MD2-SBro and MD2-MBro were shown to be enzymatically active toward casein with MD2-MBro exhibited higher enzymatic activity than MD2-SBro. The 3D structures revealed that Cys-His active site position of MD2-SBro was found to be located in the inappropriate location for catalysis. Besides, the substrate-binding pocket of MD2-SBro was found to be less hydrophobic than that of MD2-MBro. Conclusion: Unique structural features around the active site of MD2-SBro and MD2-MBro might account for the discrepancy in their enzymatic activities. |
| first_indexed | 2024-03-06T03:04:33Z |
| format | Article |
| id | ums.eprints-25972 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:04:33Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ums.eprints-259722020-09-21T00:16:58Z https://eprints.ums.edu.my/id/eprint/25972/ Structural Insights into the Enzymatic Activity of Cysteine Protease Bromelain of MD2 Pineapple Rafida Razali Vijay Kumar Subbiah Cahyo Budiman Q Science (General) QK Botany Background and Objective: The MD2 pineapple contains 14 various sizes of bromelain (MD2-bromelains) ranging from 19-200 kDa which are suspected to be structurally and enzymatically varied. This study aims to compare the enzymatic activity and structural features of small and medium-sizes of MD2-bromelains, designated as MD2-SBro (19 kDa) and MD2-MBro (38 kDa), respectively. Materials and Methods: Purified recombinant MD2-SBro and MD2-MBro obtained were used in this study. The enzymatic activity of both MD2-bromelain was determined using a plate agar system with casein as a substrate. Three-dimensional (3D) structures of both MD2-bromelains were constructed under SWISS-MODEL server-based structural homology modeling and verified stereo-chemically. Results: The MD2-SBro and MD2-MBro were shown to be enzymatically active toward casein with MD2-MBro exhibited higher enzymatic activity than MD2-SBro. The 3D structures revealed that Cys-His active site position of MD2-SBro was found to be located in the inappropriate location for catalysis. Besides, the substrate-binding pocket of MD2-SBro was found to be less hydrophobic than that of MD2-MBro. Conclusion: Unique structural features around the active site of MD2-SBro and MD2-MBro might account for the discrepancy in their enzymatic activities. 2020 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/25972/1/Structural%20Insights%20into%20the%20Enzymatic%20Activity%20of%20Cysteine%20Protease%20Bromelain%20of%20MD2%20Pineapple.pdf text en https://eprints.ums.edu.my/id/eprint/25972/2/Structural%20Insights%20into%20the%20Enzymatic%20Activity%20of%20Cysteine%20Protease%20Bromelain%20of%20MD2%20Pineapple1.pdf Rafida Razali and Vijay Kumar Subbiah and Cahyo Budiman (2020) Structural Insights into the Enzymatic Activity of Cysteine Protease Bromelain of MD2 Pineapple. Pakistan Journal of Biological Sciences, 23 (6). pp. 829-838. ISSN 1028-8880 https://doi.org/10.3923/pjbs.2020.829.838 |
| spellingShingle | Q Science (General) QK Botany Rafida Razali Vijay Kumar Subbiah Cahyo Budiman Structural Insights into the Enzymatic Activity of Cysteine Protease Bromelain of MD2 Pineapple |
| title | Structural Insights into the Enzymatic Activity of Cysteine Protease Bromelain of MD2 Pineapple |
| title_full | Structural Insights into the Enzymatic Activity of Cysteine Protease Bromelain of MD2 Pineapple |
| title_fullStr | Structural Insights into the Enzymatic Activity of Cysteine Protease Bromelain of MD2 Pineapple |
| title_full_unstemmed | Structural Insights into the Enzymatic Activity of Cysteine Protease Bromelain of MD2 Pineapple |
| title_short | Structural Insights into the Enzymatic Activity of Cysteine Protease Bromelain of MD2 Pineapple |
| title_sort | structural insights into the enzymatic activity of cysteine protease bromelain of md2 pineapple |
| topic | Q Science (General) QK Botany |
| url | https://eprints.ums.edu.my/id/eprint/25972/1/Structural%20Insights%20into%20the%20Enzymatic%20Activity%20of%20Cysteine%20Protease%20Bromelain%20of%20MD2%20Pineapple.pdf https://eprints.ums.edu.my/id/eprint/25972/2/Structural%20Insights%20into%20the%20Enzymatic%20Activity%20of%20Cysteine%20Protease%20Bromelain%20of%20MD2%20Pineapple1.pdf |
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