Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells
The aim of this study was to prepare, characterize, and determine the in vitro anticancer effects of platinumdoped magnesia (Pt/MgO) nanoparticles. The chemical compositions, functional groups, and size of nanoparticles were determined using X-ray diffraction, Fourier transform infrared spectroscopy...
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| Format: | Article |
| Language: | English English |
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Elsevier Masson SAS
2021
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| Online Access: | https://eprints.ums.edu.my/id/eprint/26750/3/Oxidative%20stress%20cytotoxicity%20induced%20by%20platinum-doped%20magnesia%20nanoparticles%20in%20cancer%20cells.pdf https://eprints.ums.edu.my/id/eprint/26750/2/Oxidative%20stress%20cytotoxicity%20induced%20by%20platinum-doped%20magnesia%20nanoparticles%20in%20cancer%20cells.pdf |
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| author | Mohamed Qasim Al-Fahdawi Faris A.J. Al-Doghachi Qasim Khlaif Abdullah Ruaa Tareq Hammad Abdullah Rasedee Wisam Nabeel Ibrahim Hussah Abdullah Alshwyeh Areej A Alosaimi Sahar Khamees Aldosary Eltayeb E. M. Eid Rozita Rosli Y.H. Taufiq-Yap Nagi A. Al-Haj Mothanna Sadiq Al-Qubaisi |
| author_facet | Mohamed Qasim Al-Fahdawi Faris A.J. Al-Doghachi Qasim Khlaif Abdullah Ruaa Tareq Hammad Abdullah Rasedee Wisam Nabeel Ibrahim Hussah Abdullah Alshwyeh Areej A Alosaimi Sahar Khamees Aldosary Eltayeb E. M. Eid Rozita Rosli Y.H. Taufiq-Yap Nagi A. Al-Haj Mothanna Sadiq Al-Qubaisi |
| author_sort | Mohamed Qasim Al-Fahdawi |
| collection | UMS |
| description | The aim of this study was to prepare, characterize, and determine the in vitro anticancer effects of platinumdoped magnesia (Pt/MgO) nanoparticles. The chemical compositions, functional groups, and size of nanoparticles were determined using X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive Xray spectroscopy, transmission electron microscopy, and scanning electron microscopy. Pt/MgO nanoparticles were cuboid and in the nanosize range of 30–50 nm. The cytotoxicity of Pt/MgO nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)− 2,5-diphenyltetrazolium bromide assay on the human lung and colonic cancer cells (A549 and HT29 respectively) and normal human lung and colonic fibroblasts cells (MRC-5 and CCD-18Co repectively). The Pt/MgO nanoparticles were relatively innocuous to normal cells. Pt/MgO nanoparticles downregulated Bcl-2 and upregulated Bax and p53 tumor suppressor proteins in the cancer cells. Pt/ MgO nanoparticles also induced production of reactive oxygen species, decreased cellular glutathione level, and increased lipid peroxidation. Thus, the anticancer effects of Pt/MgO nanoparticles were attributed to the induction of oxidative stress and apoptosis. The study showed the potential of Pt/MgO nanoparticles as an anticancer compound. |
| first_indexed | 2024-03-06T03:05:59Z |
| format | Article |
| id | ums.eprints-26750 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:05:59Z |
| publishDate | 2021 |
| publisher | Elsevier Masson SAS |
| record_format | dspace |
| spelling | ums.eprints-267502021-04-15T05:54:13Z https://eprints.ums.edu.my/id/eprint/26750/ Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells Mohamed Qasim Al-Fahdawi Faris A.J. Al-Doghachi Qasim Khlaif Abdullah Ruaa Tareq Hammad Abdullah Rasedee Wisam Nabeel Ibrahim Hussah Abdullah Alshwyeh Areej A Alosaimi Sahar Khamees Aldosary Eltayeb E. M. Eid Rozita Rosli Y.H. Taufiq-Yap Nagi A. Al-Haj Mothanna Sadiq Al-Qubaisi R Medicine (General) The aim of this study was to prepare, characterize, and determine the in vitro anticancer effects of platinumdoped magnesia (Pt/MgO) nanoparticles. The chemical compositions, functional groups, and size of nanoparticles were determined using X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive Xray spectroscopy, transmission electron microscopy, and scanning electron microscopy. Pt/MgO nanoparticles were cuboid and in the nanosize range of 30–50 nm. The cytotoxicity of Pt/MgO nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)− 2,5-diphenyltetrazolium bromide assay on the human lung and colonic cancer cells (A549 and HT29 respectively) and normal human lung and colonic fibroblasts cells (MRC-5 and CCD-18Co repectively). The Pt/MgO nanoparticles were relatively innocuous to normal cells. Pt/MgO nanoparticles downregulated Bcl-2 and upregulated Bax and p53 tumor suppressor proteins in the cancer cells. Pt/ MgO nanoparticles also induced production of reactive oxygen species, decreased cellular glutathione level, and increased lipid peroxidation. Thus, the anticancer effects of Pt/MgO nanoparticles were attributed to the induction of oxidative stress and apoptosis. The study showed the potential of Pt/MgO nanoparticles as an anticancer compound. Elsevier Masson SAS 2021-03-18 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/26750/3/Oxidative%20stress%20cytotoxicity%20induced%20by%20platinum-doped%20magnesia%20nanoparticles%20in%20cancer%20cells.pdf text en https://eprints.ums.edu.my/id/eprint/26750/2/Oxidative%20stress%20cytotoxicity%20induced%20by%20platinum-doped%20magnesia%20nanoparticles%20in%20cancer%20cells.pdf Mohamed Qasim Al-Fahdawi and Faris A.J. Al-Doghachi and Qasim Khlaif Abdullah and Ruaa Tareq Hammad and Abdullah Rasedee and Wisam Nabeel Ibrahim and Hussah Abdullah Alshwyeh and Areej A Alosaimi and Sahar Khamees Aldosary and Eltayeb E. M. Eid and Rozita Rosli and Y.H. Taufiq-Yap and Nagi A. Al-Haj and Mothanna Sadiq Al-Qubaisi (2021) Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells. Biomedicine & Pharmacotherapy, 138. pp. 1-11. https://doi.org/10.1016/j.biopha.2021.111483 |
| spellingShingle | R Medicine (General) Mohamed Qasim Al-Fahdawi Faris A.J. Al-Doghachi Qasim Khlaif Abdullah Ruaa Tareq Hammad Abdullah Rasedee Wisam Nabeel Ibrahim Hussah Abdullah Alshwyeh Areej A Alosaimi Sahar Khamees Aldosary Eltayeb E. M. Eid Rozita Rosli Y.H. Taufiq-Yap Nagi A. Al-Haj Mothanna Sadiq Al-Qubaisi Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells |
| title | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells |
| title_full | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells |
| title_fullStr | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells |
| title_full_unstemmed | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells |
| title_short | Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells |
| title_sort | oxidative stress cytotoxicity induced by platinum doped magnesia nanoparticles in cancer cells |
| topic | R Medicine (General) |
| url | https://eprints.ums.edu.my/id/eprint/26750/3/Oxidative%20stress%20cytotoxicity%20induced%20by%20platinum-doped%20magnesia%20nanoparticles%20in%20cancer%20cells.pdf https://eprints.ums.edu.my/id/eprint/26750/2/Oxidative%20stress%20cytotoxicity%20induced%20by%20platinum-doped%20magnesia%20nanoparticles%20in%20cancer%20cells.pdf |
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