Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense
There have been relatively few studies which support a link between Ganoderma boninense, a phytopathogenic fungus that is particularly cytotoxic and pathogenic to plant tissues and roots, and antimicrobial compounds. We previously observed that liquid-liquid extraction (LLE) using chloroformmethanol...
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| Format: | Article |
| Language: | English English |
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The Korean Society for Mocrobiology
2020
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| Online Access: | https://eprints.ums.edu.my/id/eprint/26839/1/Biophysical%20characterization%20of%20antibacterial%20compounds%20derived%20from%20pathogenic%20fungi%20Ganoderma%20boninense-ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/26839/2/Biophysical%20characterization%20of%20antibacterial%20compounds%20derived%20from%20pathogenic%20fungi%20Ganoderma%20boninense.pdf |
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| author | Syahriel Abdullah Yoon, Sin Oh Min-Kyu Kwak Khim Phin Chong, Khim Phin |
| author_facet | Syahriel Abdullah Yoon, Sin Oh Min-Kyu Kwak Khim Phin Chong, Khim Phin |
| author_sort | Syahriel Abdullah |
| collection | UMS |
| description | There have been relatively few studies which support a link between Ganoderma boninense, a phytopathogenic fungus that is particularly cytotoxic and pathogenic to plant tissues and roots, and antimicrobial compounds. We previously observed that liquid-liquid extraction (LLE) using chloroformmethanol-water at a ratio (1:1:1) was superior at detecting antibacterial activities and significant quantities of antibacterial compounds. Herein, we demonstrate that antibacterial secondary metabolites are produced from G. boninense mycelia. Antibacterial compounds were monitored in concurrent biochemical and biophysical experiments. The combined methods included high performance thin-layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR) spectroscopy. The antibacterial compounds derived from mycelia with chloroform-methanol extraction through LLE were isolated via a gradient solvent elution system using HPTLC. The antibacterial activity of the isolated compounds was observed to be the most potent against Staphylococcus aureus ATCC 25923 and multidrug-resistant S. aureus NCTC 11939. GC-MS, HPLC, and FTIR analysis confirmed two antibacterial compounds, which were identified as 4,4,14α-trimethylcholestane (m/z = 414.75; lanostane, C30H54) and ergosta-5,7,22-trien-3β-ol (m/z = 396.65; ergosterol, C28H44O). With the aid of spectroscopic evaluations, ganoboninketal (m/z = 498.66, C30H42O6), which belongs to the 3,4-seco-27-norlanostane triterpene family, was additionally characterized by 2D-NMR analysis. Despite the lack of antibacterial potential exhibited by lanostane; both ergosterol and ganoboninketal displayed significant antibacterial activities against bacterial pathogens. Results provide evidence for the existence of bioactive compounds in the mycelia of the relatively unexplored phytopathogenic G. boninense, together with a robust method for estimating the corresponding potent antibacterial secondary metabolites |
| first_indexed | 2024-03-06T03:06:13Z |
| format | Article |
| id | ums.eprints-26839 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:06:13Z |
| publishDate | 2020 |
| publisher | The Korean Society for Mocrobiology |
| record_format | dspace |
| spelling | ums.eprints-268392021-04-29T00:14:32Z https://eprints.ums.edu.my/id/eprint/26839/ Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense Syahriel Abdullah Yoon, Sin Oh Min-Kyu Kwak Khim Phin Chong, Khim Phin Q Science (General) SB Plant culture There have been relatively few studies which support a link between Ganoderma boninense, a phytopathogenic fungus that is particularly cytotoxic and pathogenic to plant tissues and roots, and antimicrobial compounds. We previously observed that liquid-liquid extraction (LLE) using chloroformmethanol-water at a ratio (1:1:1) was superior at detecting antibacterial activities and significant quantities of antibacterial compounds. Herein, we demonstrate that antibacterial secondary metabolites are produced from G. boninense mycelia. Antibacterial compounds were monitored in concurrent biochemical and biophysical experiments. The combined methods included high performance thin-layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR) spectroscopy. The antibacterial compounds derived from mycelia with chloroform-methanol extraction through LLE were isolated via a gradient solvent elution system using HPTLC. The antibacterial activity of the isolated compounds was observed to be the most potent against Staphylococcus aureus ATCC 25923 and multidrug-resistant S. aureus NCTC 11939. GC-MS, HPLC, and FTIR analysis confirmed two antibacterial compounds, which were identified as 4,4,14α-trimethylcholestane (m/z = 414.75; lanostane, C30H54) and ergosta-5,7,22-trien-3β-ol (m/z = 396.65; ergosterol, C28H44O). With the aid of spectroscopic evaluations, ganoboninketal (m/z = 498.66, C30H42O6), which belongs to the 3,4-seco-27-norlanostane triterpene family, was additionally characterized by 2D-NMR analysis. Despite the lack of antibacterial potential exhibited by lanostane; both ergosterol and ganoboninketal displayed significant antibacterial activities against bacterial pathogens. Results provide evidence for the existence of bioactive compounds in the mycelia of the relatively unexplored phytopathogenic G. boninense, together with a robust method for estimating the corresponding potent antibacterial secondary metabolites The Korean Society for Mocrobiology 2020-12-23 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/26839/1/Biophysical%20characterization%20of%20antibacterial%20compounds%20derived%20from%20pathogenic%20fungi%20Ganoderma%20boninense-ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/26839/2/Biophysical%20characterization%20of%20antibacterial%20compounds%20derived%20from%20pathogenic%20fungi%20Ganoderma%20boninense.pdf Syahriel Abdullah and Yoon, Sin Oh and Min-Kyu Kwak and Khim Phin Chong, Khim Phin (2020) Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense. Journal of Microbiology, 59. pp. 164-174. ISSN 1976-3794 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85099517886&origin=inward |
| spellingShingle | Q Science (General) SB Plant culture Syahriel Abdullah Yoon, Sin Oh Min-Kyu Kwak Khim Phin Chong, Khim Phin Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense |
| title | Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense |
| title_full | Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense |
| title_fullStr | Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense |
| title_full_unstemmed | Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense |
| title_short | Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense |
| title_sort | biophysical characterization of antibacterial compounds derived from pathogenic fungi ganoderma boninense |
| topic | Q Science (General) SB Plant culture |
| url | https://eprints.ums.edu.my/id/eprint/26839/1/Biophysical%20characterization%20of%20antibacterial%20compounds%20derived%20from%20pathogenic%20fungi%20Ganoderma%20boninense-ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/26839/2/Biophysical%20characterization%20of%20antibacterial%20compounds%20derived%20from%20pathogenic%20fungi%20Ganoderma%20boninense.pdf |
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