Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells
Human Wharton’s jelly mesenchymal stem cells (WJ-MSCs) treatments are being tested clinically for a range of disorders. Surface modification techniques have been instrumental in the development of biomaterials that promote cell-surface interactions. In this study, the surface of graphene oxide (GO)...
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| Format: | Thesis |
| Language: | English |
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2018
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| Online Access: | https://eprints.ums.edu.my/id/eprint/26953/1/Fabrication%20of%20Graphene%20Oxide%20and%20Graphene-OxidePeptide%20Biofilms%20characterization%2C%20cell%20viability%20and%20differentiation%20potential%20of%20Wharton%E2%80%99s%20Jelly%20Mesenchymal%20stem%20cells.pdf |
| _version_ | 1825714082302394368 |
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| author | Puah, Perng Yang |
| author_facet | Puah, Perng Yang |
| author_sort | Puah, Perng Yang |
| collection | UMS |
| description | Human Wharton’s jelly mesenchymal stem cells (WJ-MSCs) treatments are being tested clinically for a range of disorders. Surface modification techniques have been instrumental in the development of biomaterials that promote cell-surface interactions. In this study, the surface of graphene oxide (GO) was modified to promote the proliferation and differentiation of Wharton’s jelly mesenchymal stem cells (WJ-MSCs). Synthesized GO was prepared through modified Hummers method, fabrication of GO film using drop-casting method and attachment of peptide to GO film through non-covalent approach, π-π and electrostatic interactions. Synthesized GO were confirmed by UV-vis, XRD and FTIR. SEM and AFM images showed that synthesized GO has curled transparent thin film with thickness of 1.10 nm. Four peptide sequences, namely Pep1 (“N”-YIGSRWYQNMIRIKVAV-“C”), Pep2 (“N”-QHREDGSYIGSRIKVAV-“C”), Pep3 (“N”-WQPPRARIYIGSRIKVAV-“C”) and Pep4 (“N”-DGEARGDSPKRSR-“C”) were designed based short peptide sequences derived from extracellular matrix (ECM) adhesion peptides. AFM results revealed the thickness of GO biofilm (0.25 mg/mL) was 82.6 nm ± 10.4 nm, corresponding to 65 – 85 layers of single layer GO. The GO biofilm (0.25 mg/mL) treated with Pep1 shows decrease in thickness as compared to non-treated GO film and the present of peptide bond in GO/Pep biofilm was confirmed by modified Lowry method. Furthermore, GO biofilms with concentration lower than 0.25 mg/mL were able to maintain the cell viability at day 5 as compared to glass coverslip. The WJ-MSCs were able to attach and growth on GO film. Increased of cell viability at day 6 was observed for all the GO/Pep biofilms as compared to GO biofilm. GO and GO/Pep biofilm allowed WJ-MSCs attachment, proliferation and increased in osteogenic differentiation capacity. Besides, the cell cultured on GO and GO/Pep biofilm able to maintain its undifferentiated stem cell characteristic. The data obtained here collectively demonstrates that the GO/Peptide biofilm assemble via non-covalent approach is a potential substrate for the adhesion, proliferation and enhance osteogenic differentiation of human WJ-MSCs. In conclusion, GO/Pep biofilms can be utilized for designing and manipulating biomaterials for stem cell, biological and tissue engineering applications. |
| first_indexed | 2024-03-06T03:06:31Z |
| format | Thesis |
| id | ums.eprints-26953 |
| institution | Universiti Malaysia Sabah |
| language | English |
| last_indexed | 2024-03-06T03:06:31Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ums.eprints-269532021-06-02T03:17:40Z https://eprints.ums.edu.my/id/eprint/26953/ Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells Puah, Perng Yang QC Physics Human Wharton’s jelly mesenchymal stem cells (WJ-MSCs) treatments are being tested clinically for a range of disorders. Surface modification techniques have been instrumental in the development of biomaterials that promote cell-surface interactions. In this study, the surface of graphene oxide (GO) was modified to promote the proliferation and differentiation of Wharton’s jelly mesenchymal stem cells (WJ-MSCs). Synthesized GO was prepared through modified Hummers method, fabrication of GO film using drop-casting method and attachment of peptide to GO film through non-covalent approach, π-π and electrostatic interactions. Synthesized GO were confirmed by UV-vis, XRD and FTIR. SEM and AFM images showed that synthesized GO has curled transparent thin film with thickness of 1.10 nm. Four peptide sequences, namely Pep1 (“N”-YIGSRWYQNMIRIKVAV-“C”), Pep2 (“N”-QHREDGSYIGSRIKVAV-“C”), Pep3 (“N”-WQPPRARIYIGSRIKVAV-“C”) and Pep4 (“N”-DGEARGDSPKRSR-“C”) were designed based short peptide sequences derived from extracellular matrix (ECM) adhesion peptides. AFM results revealed the thickness of GO biofilm (0.25 mg/mL) was 82.6 nm ± 10.4 nm, corresponding to 65 – 85 layers of single layer GO. The GO biofilm (0.25 mg/mL) treated with Pep1 shows decrease in thickness as compared to non-treated GO film and the present of peptide bond in GO/Pep biofilm was confirmed by modified Lowry method. Furthermore, GO biofilms with concentration lower than 0.25 mg/mL were able to maintain the cell viability at day 5 as compared to glass coverslip. The WJ-MSCs were able to attach and growth on GO film. Increased of cell viability at day 6 was observed for all the GO/Pep biofilms as compared to GO biofilm. GO and GO/Pep biofilm allowed WJ-MSCs attachment, proliferation and increased in osteogenic differentiation capacity. Besides, the cell cultured on GO and GO/Pep biofilm able to maintain its undifferentiated stem cell characteristic. The data obtained here collectively demonstrates that the GO/Peptide biofilm assemble via non-covalent approach is a potential substrate for the adhesion, proliferation and enhance osteogenic differentiation of human WJ-MSCs. In conclusion, GO/Pep biofilms can be utilized for designing and manipulating biomaterials for stem cell, biological and tissue engineering applications. 2018 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/26953/1/Fabrication%20of%20Graphene%20Oxide%20and%20Graphene-OxidePeptide%20Biofilms%20characterization%2C%20cell%20viability%20and%20differentiation%20potential%20of%20Wharton%E2%80%99s%20Jelly%20Mesenchymal%20stem%20cells.pdf Puah, Perng Yang (2018) Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells. Masters thesis, Universiti Malaysia Sabah. |
| spellingShingle | QC Physics Puah, Perng Yang Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells |
| title | Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells |
| title_full | Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells |
| title_fullStr | Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells |
| title_full_unstemmed | Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells |
| title_short | Fabrication of Graphene Oxide and Graphene-Oxide/Peptide Biofilms: characterization, cell viability and differentiation potential of Wharton’s Jelly Mesenchymal stem cells |
| title_sort | fabrication of graphene oxide and graphene oxide peptide biofilms characterization cell viability and differentiation potential of wharton s jelly mesenchymal stem cells |
| topic | QC Physics |
| url | https://eprints.ums.edu.my/id/eprint/26953/1/Fabrication%20of%20Graphene%20Oxide%20and%20Graphene-OxidePeptide%20Biofilms%20characterization%2C%20cell%20viability%20and%20differentiation%20potential%20of%20Wharton%E2%80%99s%20Jelly%20Mesenchymal%20stem%20cells.pdf |
| work_keys_str_mv | AT puahperngyang fabricationofgrapheneoxideandgrapheneoxidepeptidebiofilmscharacterizationcellviabilityanddifferentiationpotentialofwhartonsjellymesenchymalstemcells |