The assessment of Cholinesterase from the liver of Puntius Javanicus as detection of metal ions
Crude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B. S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 μmole/min/mg and 0.23 mM...
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| Format: | Article |
| Language: | English English |
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Hindawi Publishing Corporation
2014
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| Online Access: | https://eprints.ums.edu.my/id/eprint/27463/1/The%20assessment%20of%20Cholinesterase%20from%20the%20liver%20of%20Puntius%20Javanicus%20as%20detection%20of%20metal%20ions%20ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/27463/2/The%20assessment%20of%20Cholinesterase%20from%20the%20liver%20of%20Puntius%20Javanicus%20as%20detection%20of%20metal%20ions%20FULL%20TEXT.pdf |
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| author | Mohd Khalizan Sabullah Mohd Rosni Sulaiman Mohd Yunus Abd Shukor Mohd Arif Syed Nor Aripin Shamaan Ariff Khalid Siti Aqlima Ahmad |
| author_facet | Mohd Khalizan Sabullah Mohd Rosni Sulaiman Mohd Yunus Abd Shukor Mohd Arif Syed Nor Aripin Shamaan Ariff Khalid Siti Aqlima Ahmad |
| author_sort | Mohd Khalizan Sabullah |
| collection | UMS |
| description | Crude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B. S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 μmole/min/mg and 0.23 mM, respectively, with catalytic efficiency ratio of 0.23. The optimum parameter was obtained at pH 7.5 and optimal temperature in the range of 25 to 30°C. The effect of different storage condition was assessed where ChE activity was significantly decreased after 9 days of storage at room temperature. However, ChE activity showed no significant difference when stored at 4.0, 0, and -25°C for 15 days. Screening of heavy metals shows that chromium, copper, and mercury strongly inhibited P. javanicus ChE by lowering the activity below 50%, while several pairwise combination of metal ions exhibited synergistic inhibiting effects on the enzyme which is greater than single exposure especially chromium, copper, and mercury. The results showed that P. javanicus ChE has the potential to be used as a biosensor for the detection of metal ions. |
| first_indexed | 2024-03-06T03:07:41Z |
| format | Article |
| id | ums.eprints-27463 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:07:41Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | ums.eprints-274632021-06-28T23:09:10Z https://eprints.ums.edu.my/id/eprint/27463/ The assessment of Cholinesterase from the liver of Puntius Javanicus as detection of metal ions Mohd Khalizan Sabullah Mohd Rosni Sulaiman Mohd Yunus Abd Shukor Mohd Arif Syed Nor Aripin Shamaan Ariff Khalid Siti Aqlima Ahmad QD Chemistry SH Aquaculture. Fisheries. Angling Crude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B. S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 μmole/min/mg and 0.23 mM, respectively, with catalytic efficiency ratio of 0.23. The optimum parameter was obtained at pH 7.5 and optimal temperature in the range of 25 to 30°C. The effect of different storage condition was assessed where ChE activity was significantly decreased after 9 days of storage at room temperature. However, ChE activity showed no significant difference when stored at 4.0, 0, and -25°C for 15 days. Screening of heavy metals shows that chromium, copper, and mercury strongly inhibited P. javanicus ChE by lowering the activity below 50%, while several pairwise combination of metal ions exhibited synergistic inhibiting effects on the enzyme which is greater than single exposure especially chromium, copper, and mercury. The results showed that P. javanicus ChE has the potential to be used as a biosensor for the detection of metal ions. Hindawi Publishing Corporation 2014 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/27463/1/The%20assessment%20of%20Cholinesterase%20from%20the%20liver%20of%20Puntius%20Javanicus%20as%20detection%20of%20metal%20ions%20ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/27463/2/The%20assessment%20of%20Cholinesterase%20from%20the%20liver%20of%20Puntius%20Javanicus%20as%20detection%20of%20metal%20ions%20FULL%20TEXT.pdf Mohd Khalizan Sabullah and Mohd Rosni Sulaiman and Mohd Yunus Abd Shukor and Mohd Arif Syed and Nor Aripin Shamaan and Ariff Khalid and Siti Aqlima Ahmad (2014) The assessment of Cholinesterase from the liver of Puntius Javanicus as detection of metal ions. The Scientific World Journal, 2014. pp. 1-9. ISSN 1537-744X https://downloads.hindawi.com/journals/tswj/2014/571094.pdf |
| spellingShingle | QD Chemistry SH Aquaculture. Fisheries. Angling Mohd Khalizan Sabullah Mohd Rosni Sulaiman Mohd Yunus Abd Shukor Mohd Arif Syed Nor Aripin Shamaan Ariff Khalid Siti Aqlima Ahmad The assessment of Cholinesterase from the liver of Puntius Javanicus as detection of metal ions |
| title | The assessment of Cholinesterase from the liver of Puntius Javanicus as detection of metal ions |
| title_full | The assessment of Cholinesterase from the liver of Puntius Javanicus as detection of metal ions |
| title_fullStr | The assessment of Cholinesterase from the liver of Puntius Javanicus as detection of metal ions |
| title_full_unstemmed | The assessment of Cholinesterase from the liver of Puntius Javanicus as detection of metal ions |
| title_short | The assessment of Cholinesterase from the liver of Puntius Javanicus as detection of metal ions |
| title_sort | assessment of cholinesterase from the liver of puntius javanicus as detection of metal ions |
| topic | QD Chemistry SH Aquaculture. Fisheries. Angling |
| url | https://eprints.ums.edu.my/id/eprint/27463/1/The%20assessment%20of%20Cholinesterase%20from%20the%20liver%20of%20Puntius%20Javanicus%20as%20detection%20of%20metal%20ions%20ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/27463/2/The%20assessment%20of%20Cholinesterase%20from%20the%20liver%20of%20Puntius%20Javanicus%20as%20detection%20of%20metal%20ions%20FULL%20TEXT.pdf |
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