Evaluation and comparison of Chlorohexidine gluconate bathing solution and antiseptic wipes antimicrobial effects against bacteria causing nosocomial infections
Nosocomial infections among critical patients in intensive care units are associated with significant morbidity and mortality globally, including in Malaysia. Both Chlorhexidine gluconate (CHG) bathing solution and antiseptic wipes were used in preoperative preparation. A controversy was reported on...
| _version_ | 1796910721519321088 |
|---|---|
| author | Hassanain Al-Talib Nurul Asyiqin Rihzam Chandrika Murugaiah |
| author_facet | Hassanain Al-Talib Nurul Asyiqin Rihzam Chandrika Murugaiah |
| author_sort | Hassanain Al-Talib |
| collection | UMS |
| description | Nosocomial infections among critical patients in intensive care units are associated with significant morbidity and mortality globally, including in Malaysia. Both Chlorhexidine gluconate (CHG) bathing solution and antiseptic wipes were used in preoperative preparation. A controversy was reported on the inhibitory effects of antiseptic wipes and CHG bathing solution on the bacteria causing nosocomial infections. This study was conducted to evaluate the antimicrobial effects of the antiseptic wipes and CHG bathing solution on nosocomial bacteria (methicillin-resistant S. aureus (MRSA), Acinetobacter baumannii, Escherichia coli, Klebsiella spp. and Pseudomonas aeruginosa). The antibacterial effectiveness of antiseptic wipes impregnated with 2% CHG and bathing solution 4% CHG were assessed using agar well diffusion method. Microtiter plate assay was used to estimate the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). The CHG from antiseptic wipes was aseptically extracted by squeezing method. The CHG bathing solution revealed excellent inhibitory effects against all study bacteria with inhibition zones [15 mm (A. Baumannii), 17 mm (Klebsiella spp.), 20 mm (Pseudomonas aeruginosa), 23 mm (Escherichia coli) and 25 mm (MRSA). In contrast, antiseptic wipes were effective against E. coli only with 15 mm inhibition zone. The MIC of CHG bathing solution was 0.03% for all study bacteria except for P. aeruginosa, which was 0.06%; however, the MIC of the antiseptic wipes against MRSA and E. coli were 0.13% and 0.5%, respectively. The MBC of CHG bathing solution against MRSA, A. baumannii and E. coli were 4%, 0.25% and 0.5% respectively. The MBC of antiseptic wipes couldn’t be determined since all study bacteria showed uncountable colonies even with the highest concentrations. In conclusion, CHG bathing solution showed a stronger antibacterial effect than antiseptic wipes against nosocomial bacteria. Using CHG bathing solution will significantly reduce the risks of acquiring multidrug resistant organisms and developing nosocomial infections. |
| first_indexed | 2024-03-06T03:08:28Z |
| format | Article |
| id | ums.eprints-29019 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:08:28Z |
| publishDate | 2022 |
| publisher | Penerbit UMT |
| record_format | dspace |
| spelling | ums.eprints-290192022-08-01T00:29:55Z https://eprints.ums.edu.my/id/eprint/29019/ Evaluation and comparison of Chlorohexidine gluconate bathing solution and antiseptic wipes antimicrobial effects against bacteria causing nosocomial infections Hassanain Al-Talib Nurul Asyiqin Rihzam Chandrika Murugaiah QR1-502 Microbiology RM300-666 Drugs and their actions Nosocomial infections among critical patients in intensive care units are associated with significant morbidity and mortality globally, including in Malaysia. Both Chlorhexidine gluconate (CHG) bathing solution and antiseptic wipes were used in preoperative preparation. A controversy was reported on the inhibitory effects of antiseptic wipes and CHG bathing solution on the bacteria causing nosocomial infections. This study was conducted to evaluate the antimicrobial effects of the antiseptic wipes and CHG bathing solution on nosocomial bacteria (methicillin-resistant S. aureus (MRSA), Acinetobacter baumannii, Escherichia coli, Klebsiella spp. and Pseudomonas aeruginosa). The antibacterial effectiveness of antiseptic wipes impregnated with 2% CHG and bathing solution 4% CHG were assessed using agar well diffusion method. Microtiter plate assay was used to estimate the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). The CHG from antiseptic wipes was aseptically extracted by squeezing method. The CHG bathing solution revealed excellent inhibitory effects against all study bacteria with inhibition zones [15 mm (A. Baumannii), 17 mm (Klebsiella spp.), 20 mm (Pseudomonas aeruginosa), 23 mm (Escherichia coli) and 25 mm (MRSA). In contrast, antiseptic wipes were effective against E. coli only with 15 mm inhibition zone. The MIC of CHG bathing solution was 0.03% for all study bacteria except for P. aeruginosa, which was 0.06%; however, the MIC of the antiseptic wipes against MRSA and E. coli were 0.13% and 0.5%, respectively. The MBC of CHG bathing solution against MRSA, A. baumannii and E. coli were 4%, 0.25% and 0.5% respectively. The MBC of antiseptic wipes couldn’t be determined since all study bacteria showed uncountable colonies even with the highest concentrations. In conclusion, CHG bathing solution showed a stronger antibacterial effect than antiseptic wipes against nosocomial bacteria. Using CHG bathing solution will significantly reduce the risks of acquiring multidrug resistant organisms and developing nosocomial infections. Penerbit UMT 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/29019/2/Evaluation%20and%20comparison%20of%20Chlorohexidine%20gluconate%20bathing%20solution%20and%20antiseptic%20wipes%20antimicrobial%20effects%20against%20bacteria%20causing%20nosocomial%20infections.pdf text en https://eprints.ums.edu.my/id/eprint/29019/3/Evaluation%20and%20comparison%20of%20Chlorohexidine%20gluconate%20bathing%20solution%20and%20antiseptic%20wipes%20antimicrobial%20effects%20against%20bacteria%20causing%20nosocomial%20infections%20_ABSTRACT.pdf Hassanain Al-Talib and Nurul Asyiqin Rihzam and Chandrika Murugaiah (2022) Evaluation and comparison of Chlorohexidine gluconate bathing solution and antiseptic wipes antimicrobial effects against bacteria causing nosocomial infections. Journal of Sustainability Science and Management, 17. pp. 187-193. ISSN 1823-8556 (P-ISSN) , 2672-7226 (E-ISSN) https://jssm.umt.edu.my/wp-content/uploads/sites/51/2022/04/12.-EVALUATION-AND-COMPARISON-OF-CHLORHEXIDINE-GLUCONATE-1.pdf http://doi.org/10.46754/jssm.2022.01.012 http://doi.org/10.46754/jssm.2022.01.012 |
| spellingShingle | QR1-502 Microbiology RM300-666 Drugs and their actions Hassanain Al-Talib Nurul Asyiqin Rihzam Chandrika Murugaiah Evaluation and comparison of Chlorohexidine gluconate bathing solution and antiseptic wipes antimicrobial effects against bacteria causing nosocomial infections |
| title | Evaluation and comparison of Chlorohexidine gluconate bathing solution and antiseptic wipes antimicrobial effects against bacteria causing nosocomial infections |
| title_full | Evaluation and comparison of Chlorohexidine gluconate bathing solution and antiseptic wipes antimicrobial effects against bacteria causing nosocomial infections |
| title_fullStr | Evaluation and comparison of Chlorohexidine gluconate bathing solution and antiseptic wipes antimicrobial effects against bacteria causing nosocomial infections |
| title_full_unstemmed | Evaluation and comparison of Chlorohexidine gluconate bathing solution and antiseptic wipes antimicrobial effects against bacteria causing nosocomial infections |
| title_short | Evaluation and comparison of Chlorohexidine gluconate bathing solution and antiseptic wipes antimicrobial effects against bacteria causing nosocomial infections |
| title_sort | evaluation and comparison of chlorohexidine gluconate bathing solution and antiseptic wipes antimicrobial effects against bacteria causing nosocomial infections |
| topic | QR1-502 Microbiology RM300-666 Drugs and their actions |
| url | https://eprints.ums.edu.my/id/eprint/29019/2/Evaluation%20and%20comparison%20of%20Chlorohexidine%20gluconate%20bathing%20solution%20and%20antiseptic%20wipes%20antimicrobial%20effects%20against%20bacteria%20causing%20nosocomial%20infections.pdf https://eprints.ums.edu.my/id/eprint/29019/3/Evaluation%20and%20comparison%20of%20Chlorohexidine%20gluconate%20bathing%20solution%20and%20antiseptic%20wipes%20antimicrobial%20effects%20against%20bacteria%20causing%20nosocomial%20infections%20_ABSTRACT.pdf |
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