Surface characterization, biocompatibility and osteogenic differentiation of drop-casted multilayer graphene oxide film towards human Wharton’s jelly derived mesenchymal stem cells

Graphene oxide (GO) materials have been extensively employed in mesenchymal stem cell (MSCs) research due to its unique nano topography. Herein, various concentrations of GO flakes were used to fabricate different thickness of multilayer graphene oxide (m-GO) films using a simple drop casting method...

Full description

Bibliographic Details
Main Authors: Perng Yang Puah, Umul Hanim Yusof, Lee, Ping Chin, Moh, Pak Yan, How, Siew Eng
Format: Article
Language:English
English
Published: Taylor & Francis Group 2020
Subjects:
Online Access:https://eprints.ums.edu.my/id/eprint/30157/1/Surface%20characterization%2C%20biocompatibility%20and%20osteogenic%20differentiation%20of%20drop-casted%20multilayer%20graphene%20oxide%20film%20towards%20human%20Wharton%E2%80%99s%20jelly%20derived%20mesenchymal%20stem%20cells.pdf
https://eprints.ums.edu.my/id/eprint/30157/2/Surface%20characterization%2C%20biocompatibility%20and%20osteogenic%20differentiation%20of%20drop-casted%20multilayer%20graphene%20oxide%20film%20towards%20human%20Wharton%E2%80%99s%20jelly%20derived%20mesenchymal%20stem%20cells1.pdf
_version_ 1825714306190147584
author Perng Yang Puah
Umul Hanim Yusof
Lee, Ping Chin
Moh, Pak Yan
How, Siew Eng
author_facet Perng Yang Puah
Umul Hanim Yusof
Lee, Ping Chin
Moh, Pak Yan
How, Siew Eng
author_sort Perng Yang Puah
collection UMS
description Graphene oxide (GO) materials have been extensively employed in mesenchymal stem cell (MSCs) research due to its unique nano topography. Herein, various concentrations of GO flakes were used to fabricate different thickness of multilayer graphene oxide (m-GO) films using a simple drop casting method and characterized by FTIR and AFM. The biocompatibility of m-GO films in culturing WJ-MSCs was investigated based on cell morphology, cell viability and osteogenic differentiation ability. Importantly, WJ-MSCs adhered and proliferated successfully on the m-GO films (6.25 µg, 12.5 µg and 25 µg) and showed no difference in cell morphology and viability after 5 days culture. Moreover, the WJ-MSCs growth on GO films (6.25 µg, 12.5 µg and 25 µg) enhanced the osteogenic differentiation as compared to the control (glass coverslip). Hence, the simple and inexpensive drop-casted fabrication strategy could provide biocompatible m-GO films to unlock the wider potential of WJ-MCSs in tissue engineering application.
first_indexed 2024-03-06T03:09:51Z
format Article
id ums.eprints-30157
institution Universiti Malaysia Sabah
language English
English
last_indexed 2024-03-06T03:09:51Z
publishDate 2020
publisher Taylor & Francis Group
record_format dspace
spelling ums.eprints-301572021-07-31T01:14:03Z https://eprints.ums.edu.my/id/eprint/30157/ Surface characterization, biocompatibility and osteogenic differentiation of drop-casted multilayer graphene oxide film towards human Wharton’s jelly derived mesenchymal stem cells Perng Yang Puah Umul Hanim Yusof Lee, Ping Chin Moh, Pak Yan How, Siew Eng Q Science (General) QM Human anatomy Graphene oxide (GO) materials have been extensively employed in mesenchymal stem cell (MSCs) research due to its unique nano topography. Herein, various concentrations of GO flakes were used to fabricate different thickness of multilayer graphene oxide (m-GO) films using a simple drop casting method and characterized by FTIR and AFM. The biocompatibility of m-GO films in culturing WJ-MSCs was investigated based on cell morphology, cell viability and osteogenic differentiation ability. Importantly, WJ-MSCs adhered and proliferated successfully on the m-GO films (6.25 µg, 12.5 µg and 25 µg) and showed no difference in cell morphology and viability after 5 days culture. Moreover, the WJ-MSCs growth on GO films (6.25 µg, 12.5 µg and 25 µg) enhanced the osteogenic differentiation as compared to the control (glass coverslip). Hence, the simple and inexpensive drop-casted fabrication strategy could provide biocompatible m-GO films to unlock the wider potential of WJ-MCSs in tissue engineering application. Taylor & Francis Group 2020 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/30157/1/Surface%20characterization%2C%20biocompatibility%20and%20osteogenic%20differentiation%20of%20drop-casted%20multilayer%20graphene%20oxide%20film%20towards%20human%20Wharton%E2%80%99s%20jelly%20derived%20mesenchymal%20stem%20cells.pdf text en https://eprints.ums.edu.my/id/eprint/30157/2/Surface%20characterization%2C%20biocompatibility%20and%20osteogenic%20differentiation%20of%20drop-casted%20multilayer%20graphene%20oxide%20film%20towards%20human%20Wharton%E2%80%99s%20jelly%20derived%20mesenchymal%20stem%20cells1.pdf Perng Yang Puah and Umul Hanim Yusof and Lee, Ping Chin and Moh, Pak Yan and How, Siew Eng (2020) Surface characterization, biocompatibility and osteogenic differentiation of drop-casted multilayer graphene oxide film towards human Wharton’s jelly derived mesenchymal stem cells. Materials Technology, 35. pp. 238-247. ISSN 1066-7857 https://medpubresearch.com/wp-content/uploads/2020/03/Surface-characterization-biocompatibility-and-osteogenic-differentiation-of-drop-casted-multilayer-graphene-oxide-film-towards-human-whartons-jelly-derived-mesenchymal-stem-cells.pdf https://doi.org/10.1080/10667857.2019.1674506 https://doi.org/10.1080/10667857.2019.1674506
spellingShingle Q Science (General)
QM Human anatomy
Perng Yang Puah
Umul Hanim Yusof
Lee, Ping Chin
Moh, Pak Yan
How, Siew Eng
Surface characterization, biocompatibility and osteogenic differentiation of drop-casted multilayer graphene oxide film towards human Wharton’s jelly derived mesenchymal stem cells
title Surface characterization, biocompatibility and osteogenic differentiation of drop-casted multilayer graphene oxide film towards human Wharton’s jelly derived mesenchymal stem cells
title_full Surface characterization, biocompatibility and osteogenic differentiation of drop-casted multilayer graphene oxide film towards human Wharton’s jelly derived mesenchymal stem cells
title_fullStr Surface characterization, biocompatibility and osteogenic differentiation of drop-casted multilayer graphene oxide film towards human Wharton’s jelly derived mesenchymal stem cells
title_full_unstemmed Surface characterization, biocompatibility and osteogenic differentiation of drop-casted multilayer graphene oxide film towards human Wharton’s jelly derived mesenchymal stem cells
title_short Surface characterization, biocompatibility and osteogenic differentiation of drop-casted multilayer graphene oxide film towards human Wharton’s jelly derived mesenchymal stem cells
title_sort surface characterization biocompatibility and osteogenic differentiation of drop casted multilayer graphene oxide film towards human wharton s jelly derived mesenchymal stem cells
topic Q Science (General)
QM Human anatomy
url https://eprints.ums.edu.my/id/eprint/30157/1/Surface%20characterization%2C%20biocompatibility%20and%20osteogenic%20differentiation%20of%20drop-casted%20multilayer%20graphene%20oxide%20film%20towards%20human%20Wharton%E2%80%99s%20jelly%20derived%20mesenchymal%20stem%20cells.pdf
https://eprints.ums.edu.my/id/eprint/30157/2/Surface%20characterization%2C%20biocompatibility%20and%20osteogenic%20differentiation%20of%20drop-casted%20multilayer%20graphene%20oxide%20film%20towards%20human%20Wharton%E2%80%99s%20jelly%20derived%20mesenchymal%20stem%20cells1.pdf
work_keys_str_mv AT perngyangpuah surfacecharacterizationbiocompatibilityandosteogenicdifferentiationofdropcastedmultilayergrapheneoxidefilmtowardshumanwhartonsjellyderivedmesenchymalstemcells
AT umulhanimyusof surfacecharacterizationbiocompatibilityandosteogenicdifferentiationofdropcastedmultilayergrapheneoxidefilmtowardshumanwhartonsjellyderivedmesenchymalstemcells
AT leepingchin surfacecharacterizationbiocompatibilityandosteogenicdifferentiationofdropcastedmultilayergrapheneoxidefilmtowardshumanwhartonsjellyderivedmesenchymalstemcells
AT mohpakyan surfacecharacterizationbiocompatibilityandosteogenicdifferentiationofdropcastedmultilayergrapheneoxidefilmtowardshumanwhartonsjellyderivedmesenchymalstemcells
AT howsieweng surfacecharacterizationbiocompatibilityandosteogenicdifferentiationofdropcastedmultilayergrapheneoxidefilmtowardshumanwhartonsjellyderivedmesenchymalstemcells