Recombinant LipL32 protein developed using a synthetic gene detects leptospira-specific antibodies in human serum samples
Background: Synthetic biology is emerging as a viable alternative for the production of recombinant antigens for diagnostic applications. It offers a safe alternative for the synthesis of antigenic principles derived from organisms that pose a high biological risk. Methods: Here, we describe an enzy...
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| Format: | Article |
| Language: | English English |
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Universiti Sains Malaysia
2017
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| Online Access: | https://eprints.ums.edu.my/id/eprint/30245/1/Recombinant%20LipL32%20protein%20developed%20using%20a%20synthetic%20gene%20detects%20leptospira-specific%20antibodies%20in%20human%20serum%20samples%20ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/30245/2/Recombinant%20LipL32%20protein%20developed%20using%20a%20synthetic%20gene%20detects%20leptospira-specific%20antibodies%20in%20human%20serum%20samples%20FULL%20TEXT.pdf |
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| author | Yuszniahyati Yaakob Kenneth Francis Rodrigues Fernandes Opook Timothy William Daisy Vanitha John |
| author_facet | Yuszniahyati Yaakob Kenneth Francis Rodrigues Fernandes Opook Timothy William Daisy Vanitha John |
| author_sort | Yuszniahyati Yaakob |
| collection | UMS |
| description | Background: Synthetic biology is emerging as a viable alternative for the production of recombinant antigens for diagnostic applications. It offers a safe alternative for the synthesis of antigenic principles derived from organisms that pose a high biological risk. Methods: Here, we describe an enzyme-linked immunosorbent assay (ELISA) using the synthetic recombinant LipL32 (rLipL32) protein expressed in Escherichia coli for the detection of Leptospira-specific antibodies in human serum samples. The rLipL32-based ELISA was compared with a microscopic agglutination test (MAT), which is currently used as the gold standard for the diagnosis of leptospirosis. Results: Our results showed that all the MAT-positive serum samples were positive for Leptospira-specific IgG in an ELISA, while 65% (n = 13) of these samples were also positive for Leptospira-specific IgM. In the MAT-negative serum samples, 80% and 55% of the samples were detected as negative by an ELISA for Leptospira-specific IgM and IgG, respectively. Conclusion: An ELISA using the synthetic rLipL32 antigen was able to distinguish Leptospira-specific IgM (sensitivity 65% and specificity 80%) and IgG (sensitivity 100% and specificity 55%) in human serum samples and has the potential to serve as a rapid diagnostic test for leptospirosis. |
| first_indexed | 2024-03-06T03:10:02Z |
| format | Article |
| id | ums.eprints-30245 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:10:02Z |
| publishDate | 2017 |
| publisher | Universiti Sains Malaysia |
| record_format | dspace |
| spelling | ums.eprints-302452021-08-21T04:09:34Z https://eprints.ums.edu.my/id/eprint/30245/ Recombinant LipL32 protein developed using a synthetic gene detects leptospira-specific antibodies in human serum samples Yuszniahyati Yaakob Kenneth Francis Rodrigues Fernandes Opook Timothy William Daisy Vanitha John RB37-56.5 Clinical pathology. Laboratory technique Background: Synthetic biology is emerging as a viable alternative for the production of recombinant antigens for diagnostic applications. It offers a safe alternative for the synthesis of antigenic principles derived from organisms that pose a high biological risk. Methods: Here, we describe an enzyme-linked immunosorbent assay (ELISA) using the synthetic recombinant LipL32 (rLipL32) protein expressed in Escherichia coli for the detection of Leptospira-specific antibodies in human serum samples. The rLipL32-based ELISA was compared with a microscopic agglutination test (MAT), which is currently used as the gold standard for the diagnosis of leptospirosis. Results: Our results showed that all the MAT-positive serum samples were positive for Leptospira-specific IgG in an ELISA, while 65% (n = 13) of these samples were also positive for Leptospira-specific IgM. In the MAT-negative serum samples, 80% and 55% of the samples were detected as negative by an ELISA for Leptospira-specific IgM and IgG, respectively. Conclusion: An ELISA using the synthetic rLipL32 antigen was able to distinguish Leptospira-specific IgM (sensitivity 65% and specificity 80%) and IgG (sensitivity 100% and specificity 55%) in human serum samples and has the potential to serve as a rapid diagnostic test for leptospirosis. Universiti Sains Malaysia 2017 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/30245/1/Recombinant%20LipL32%20protein%20developed%20using%20a%20synthetic%20gene%20detects%20leptospira-specific%20antibodies%20in%20human%20serum%20samples%20ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/30245/2/Recombinant%20LipL32%20protein%20developed%20using%20a%20synthetic%20gene%20detects%20leptospira-specific%20antibodies%20in%20human%20serum%20samples%20FULL%20TEXT.pdf Yuszniahyati Yaakob and Kenneth Francis Rodrigues and Fernandes Opook and Timothy William and Daisy Vanitha John (2017) Recombinant LipL32 protein developed using a synthetic gene detects leptospira-specific antibodies in human serum samples. Malaysian Journal of Medical Science, 25. pp. 44-51. ISSN 1394-195X (P-ISSN) , 2180-4303 (E-ISSN) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5772814/pdf/05mjms24052017_oa3.pdf https://doi.org/10.21315/mjms2017.24.5.5 https://doi.org/10.21315/mjms2017.24.5.5 |
| spellingShingle | RB37-56.5 Clinical pathology. Laboratory technique Yuszniahyati Yaakob Kenneth Francis Rodrigues Fernandes Opook Timothy William Daisy Vanitha John Recombinant LipL32 protein developed using a synthetic gene detects leptospira-specific antibodies in human serum samples |
| title | Recombinant LipL32 protein developed using a synthetic gene detects leptospira-specific antibodies in human serum samples |
| title_full | Recombinant LipL32 protein developed using a synthetic gene detects leptospira-specific antibodies in human serum samples |
| title_fullStr | Recombinant LipL32 protein developed using a synthetic gene detects leptospira-specific antibodies in human serum samples |
| title_full_unstemmed | Recombinant LipL32 protein developed using a synthetic gene detects leptospira-specific antibodies in human serum samples |
| title_short | Recombinant LipL32 protein developed using a synthetic gene detects leptospira-specific antibodies in human serum samples |
| title_sort | recombinant lipl32 protein developed using a synthetic gene detects leptospira specific antibodies in human serum samples |
| topic | RB37-56.5 Clinical pathology. Laboratory technique |
| url | https://eprints.ums.edu.my/id/eprint/30245/1/Recombinant%20LipL32%20protein%20developed%20using%20a%20synthetic%20gene%20detects%20leptospira-specific%20antibodies%20in%20human%20serum%20samples%20ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/30245/2/Recombinant%20LipL32%20protein%20developed%20using%20a%20synthetic%20gene%20detects%20leptospira-specific%20antibodies%20in%20human%20serum%20samples%20FULL%20TEXT.pdf |
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