The anti-leech potential of the solvent extract of Bornean neem leaves and ultra-high performance liquid chromatography-high-resolution mass spectrometry profiling
Aquaculture plays a vital role in the Malaysian economy and food production however, sometimes the development is impeded by parasites. The parasitic leech Zeylanicobdella arugamensis (Hirudinea) infest the sea bass, snappers and groupers. They feed on the blood and cause secondary infection which c...
| _version_ | 1825714378271358976 |
|---|---|
| author | Muhammad Dawood Shah Balu Alagar Venmathi Maran Jen, Kit Tan Yoong, Soon Yong Ching Fui Fui @ Faihana Ching Abdullah Sitti Raehanah Muhamad Shaleh Rossita Shapawi |
| author_facet | Muhammad Dawood Shah Balu Alagar Venmathi Maran Jen, Kit Tan Yoong, Soon Yong Ching Fui Fui @ Faihana Ching Abdullah Sitti Raehanah Muhamad Shaleh Rossita Shapawi |
| author_sort | Muhammad Dawood Shah |
| collection | UMS |
| description | Aquaculture plays a vital role in the Malaysian economy and food production however, sometimes the development is impeded by parasites. The parasitic leech Zeylanicobdella arugamensis (Hirudinea) infest the sea bass, snappers and groupers. They feed on the blood and cause secondary infection which could cause host death in a short time. In this study, the leaves of the neem plant (Azadirachta indica) (Meliaceae) have been selected as a natural product for the elimination of the parasitic leeches Z. arugamensis and to evaluate the phytochemical composition via ultra-high performance liquid chromatography–high-resolution mass spectrometry system (UHPLC-HRMS). The parasitic leeches were collected from Universiti Malaysia Sabah aquaculture facilities and challenged with various concentration of neem leaves methanol extract. The results revealed significant anti leech activity of the methanol extract against Z. Arugamensis with total mortality at the concentration of 25, 50 and 100 mg/ml in an average period of 35.81 ± 5.40, 14.18 ± 0.99 and 9.21 ± 1.51 min, respectively. The analysis of UHPLC-HRMS showed the existence of isorhamnetin, myricetin, myricetin 3-O-galactoside, trifolin, and quercetin(flavonoids), 2-(3,4-dihydroxyphenyl) acetamide, apocynin, p-coumaric acid, scopoletin, and, phloretin(phenolics), pulegone, and carvone (terpenoids). Thus, the research showed that methanol extract of neem leaves contained effective bioactive compounds with anti-leech effects. This study will be very helpful to fish farmers in Malaysia and other Southeast Asian countries to control the parasitic leeches in aquaculture using a natural product. |
| first_indexed | 2024-03-06T03:10:55Z |
| format | Article |
| id | ums.eprints-30566 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:10:55Z |
| publishDate | 2021 |
| publisher | King Saud University |
| record_format | dspace |
| spelling | ums.eprints-305662021-10-22T02:47:00Z https://eprints.ums.edu.my/id/eprint/30566/ The anti-leech potential of the solvent extract of Bornean neem leaves and ultra-high performance liquid chromatography-high-resolution mass spectrometry profiling Muhammad Dawood Shah Balu Alagar Venmathi Maran Jen, Kit Tan Yoong, Soon Yong Ching Fui Fui @ Faihana Ching Abdullah Sitti Raehanah Muhamad Shaleh Rossita Shapawi SH171-179 Diseases and adverse factors Aquaculture plays a vital role in the Malaysian economy and food production however, sometimes the development is impeded by parasites. The parasitic leech Zeylanicobdella arugamensis (Hirudinea) infest the sea bass, snappers and groupers. They feed on the blood and cause secondary infection which could cause host death in a short time. In this study, the leaves of the neem plant (Azadirachta indica) (Meliaceae) have been selected as a natural product for the elimination of the parasitic leeches Z. arugamensis and to evaluate the phytochemical composition via ultra-high performance liquid chromatography–high-resolution mass spectrometry system (UHPLC-HRMS). The parasitic leeches were collected from Universiti Malaysia Sabah aquaculture facilities and challenged with various concentration of neem leaves methanol extract. The results revealed significant anti leech activity of the methanol extract against Z. Arugamensis with total mortality at the concentration of 25, 50 and 100 mg/ml in an average period of 35.81 ± 5.40, 14.18 ± 0.99 and 9.21 ± 1.51 min, respectively. The analysis of UHPLC-HRMS showed the existence of isorhamnetin, myricetin, myricetin 3-O-galactoside, trifolin, and quercetin(flavonoids), 2-(3,4-dihydroxyphenyl) acetamide, apocynin, p-coumaric acid, scopoletin, and, phloretin(phenolics), pulegone, and carvone (terpenoids). Thus, the research showed that methanol extract of neem leaves contained effective bioactive compounds with anti-leech effects. This study will be very helpful to fish farmers in Malaysia and other Southeast Asian countries to control the parasitic leeches in aquaculture using a natural product. King Saud University 2021 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/30566/1/The%20anti-leech%20potential%20of%20the%20solvent%20extract%20of%20Bornean%20neem%20leavesand%20ultra-high%20performance%20liquid%20chromatography-high-resolutionmass%20spectrometry%20profiling%20FULL%20TEXT.pdf text en https://eprints.ums.edu.my/id/eprint/30566/3/The%20anti-leech%20potential%20of%20the%20solvent%20extract%20of%20Bornean%20neem%20leaves%20and%20ultra-high%20performance%20liquid%20chromatography-high-resolution%20mass%20spectrometry%20profiling%20ABSTRACT.pdf Muhammad Dawood Shah and Balu Alagar Venmathi Maran and Jen, Kit Tan and Yoong, Soon Yong and Ching Fui Fui @ Faihana Ching Abdullah and Sitti Raehanah Muhamad Shaleh and Rossita Shapawi (2021) The anti-leech potential of the solvent extract of Bornean neem leaves and ultra-high performance liquid chromatography-high-resolution mass spectrometry profiling. Journal of King Saud University - Science, 33. pp. 1-7. ISSN 1018-3647 https://www.sciencedirect.com/science/article/pii/S1018364721002020 https://doi.org/10.1016/j.jksus.2021.101541 https://doi.org/10.1016/j.jksus.2021.101541 |
| spellingShingle | SH171-179 Diseases and adverse factors Muhammad Dawood Shah Balu Alagar Venmathi Maran Jen, Kit Tan Yoong, Soon Yong Ching Fui Fui @ Faihana Ching Abdullah Sitti Raehanah Muhamad Shaleh Rossita Shapawi The anti-leech potential of the solvent extract of Bornean neem leaves and ultra-high performance liquid chromatography-high-resolution mass spectrometry profiling |
| title | The anti-leech potential of the solvent extract of Bornean neem leaves and ultra-high performance liquid chromatography-high-resolution mass spectrometry profiling |
| title_full | The anti-leech potential of the solvent extract of Bornean neem leaves and ultra-high performance liquid chromatography-high-resolution mass spectrometry profiling |
| title_fullStr | The anti-leech potential of the solvent extract of Bornean neem leaves and ultra-high performance liquid chromatography-high-resolution mass spectrometry profiling |
| title_full_unstemmed | The anti-leech potential of the solvent extract of Bornean neem leaves and ultra-high performance liquid chromatography-high-resolution mass spectrometry profiling |
| title_short | The anti-leech potential of the solvent extract of Bornean neem leaves and ultra-high performance liquid chromatography-high-resolution mass spectrometry profiling |
| title_sort | anti leech potential of the solvent extract of bornean neem leaves and ultra high performance liquid chromatography high resolution mass spectrometry profiling |
| topic | SH171-179 Diseases and adverse factors |
| url | https://eprints.ums.edu.my/id/eprint/30566/1/The%20anti-leech%20potential%20of%20the%20solvent%20extract%20of%20Bornean%20neem%20leavesand%20ultra-high%20performance%20liquid%20chromatography-high-resolutionmass%20spectrometry%20profiling%20FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/30566/3/The%20anti-leech%20potential%20of%20the%20solvent%20extract%20of%20Bornean%20neem%20leaves%20and%20ultra-high%20performance%20liquid%20chromatography-high-resolution%20mass%20spectrometry%20profiling%20ABSTRACT.pdf |
| work_keys_str_mv | AT muhammaddawoodshah theantileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling AT balualagarvenmathimaran theantileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling AT jenkittan theantileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling AT yoongsoonyong theantileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling AT chingfuifuifaihanachingabdullah theantileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling AT sittiraehanahmuhamadshaleh theantileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling AT rossitashapawi theantileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling AT muhammaddawoodshah antileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling AT balualagarvenmathimaran antileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling AT jenkittan antileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling AT yoongsoonyong antileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling AT chingfuifuifaihanachingabdullah antileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling AT sittiraehanahmuhamadshaleh antileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling AT rossitashapawi antileechpotentialofthesolventextractofborneanneemleavesandultrahighperformanceliquidchromatographyhighresolutionmassspectrometryprofiling |