GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis
In the present study the stems of the Bridelia stipularis (L.) Blume, which is traditionally used by ethnic communities in Sabah, Malaysia, has been investigated for its chemical composition, total flavonoid content (TFC) and total phenolic content (TPC) via Gas-Chromatography-Mass Spectroscopy (GC-...
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| Format: | Article |
| Language: | English English |
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Penerbit UKM
2019
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| Online Access: | https://eprints.ums.edu.my/id/eprint/30738/1/GC-MS%20analysis%20of%20chemical%20constituents%20and%20in%20vitro%20antioxidant%20activity%20of%20the%20organic%20extracts%20from%20the%20stem%20of%20Bridelia%20stipularis-ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/30738/2/GC-MS%20analysis%20of%20chemical%20constituents%20and%20in%20vitro%20antioxidant%20activity%20of%20the%20organic%20extracts%20from%20the%20stem%20of%20Bridelia%20stipularis.pdf |
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| author | Samina Khan Yusufzai Mohammad Shaheen Khan Eunice Lua Hanry Mohd. Rafatullah Belsheera Elison |
| author_facet | Samina Khan Yusufzai Mohammad Shaheen Khan Eunice Lua Hanry Mohd. Rafatullah Belsheera Elison |
| author_sort | Samina Khan Yusufzai |
| collection | UMS |
| description | In the present study the stems of the Bridelia stipularis (L.) Blume, which is traditionally used by ethnic communities in Sabah, Malaysia, has been investigated for its chemical composition, total flavonoid content (TFC) and total phenolic content (TPC) via Gas-Chromatography-Mass Spectroscopy (GC-MS) analysis consuming hexane, chloroform and ethyl acetate as extraction solvents and gallic acid and quercetin as internal standards. In vitro antioxidant activity (AA) was determined by the application of 1,1-diphenyl-2-picryl hydrazine (DPPH) radical scavenging assay using tert-butyl-1-hydroxytoluene (BHT) as comparative drug. The GC-MS profiling showed the presence of 1-dodecanol (40.917%), oxalic acid, cyclobutyl octadecyl ester (24.985%), 1-octanol,2-nitro (12.424%), benzaldehyde, 2,4-dimethyl- (9.583%), 4-tridecanol (6.359%) and nitric acid, nonyl ester (5.616%) as major constituents. The TPC (224.62 ± 0.08 mg QE/g) and TFC (160.48 ± 0.08 mg GAE/g) was reported highest for the most polar solvent i.e. ethyl acetate. The in vitro antioxidant study disclosed highest IC50 value for ethyl acetate (2.15 mg/mL), queued by chloroform (1.19 mg/mL) and hexane (0.89 mg/mL), displaying that polar solvents are good extraction solvents for the identification of free radical scavenging properties, TFC and TPC. |
| first_indexed | 2024-03-06T03:11:19Z |
| format | Article |
| id | ums.eprints-30738 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:11:19Z |
| publishDate | 2019 |
| publisher | Penerbit UKM |
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| spelling | ums.eprints-307382021-10-26T08:04:42Z https://eprints.ums.edu.my/id/eprint/30738/ GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis Samina Khan Yusufzai Mohammad Shaheen Khan Eunice Lua Hanry Mohd. Rafatullah Belsheera Elison QD1-999 Chemistry QK710-899 Plant physiology In the present study the stems of the Bridelia stipularis (L.) Blume, which is traditionally used by ethnic communities in Sabah, Malaysia, has been investigated for its chemical composition, total flavonoid content (TFC) and total phenolic content (TPC) via Gas-Chromatography-Mass Spectroscopy (GC-MS) analysis consuming hexane, chloroform and ethyl acetate as extraction solvents and gallic acid and quercetin as internal standards. In vitro antioxidant activity (AA) was determined by the application of 1,1-diphenyl-2-picryl hydrazine (DPPH) radical scavenging assay using tert-butyl-1-hydroxytoluene (BHT) as comparative drug. The GC-MS profiling showed the presence of 1-dodecanol (40.917%), oxalic acid, cyclobutyl octadecyl ester (24.985%), 1-octanol,2-nitro (12.424%), benzaldehyde, 2,4-dimethyl- (9.583%), 4-tridecanol (6.359%) and nitric acid, nonyl ester (5.616%) as major constituents. The TPC (224.62 ± 0.08 mg QE/g) and TFC (160.48 ± 0.08 mg GAE/g) was reported highest for the most polar solvent i.e. ethyl acetate. The in vitro antioxidant study disclosed highest IC50 value for ethyl acetate (2.15 mg/mL), queued by chloroform (1.19 mg/mL) and hexane (0.89 mg/mL), displaying that polar solvents are good extraction solvents for the identification of free radical scavenging properties, TFC and TPC. Penerbit UKM 2019 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/30738/1/GC-MS%20analysis%20of%20chemical%20constituents%20and%20in%20vitro%20antioxidant%20activity%20of%20the%20organic%20extracts%20from%20the%20stem%20of%20Bridelia%20stipularis-ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/30738/2/GC-MS%20analysis%20of%20chemical%20constituents%20and%20in%20vitro%20antioxidant%20activity%20of%20the%20organic%20extracts%20from%20the%20stem%20of%20Bridelia%20stipularis.pdf Samina Khan Yusufzai and Mohammad Shaheen Khan and Eunice Lua Hanry and Mohd. Rafatullah and Belsheera Elison (2019) GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis. Sains Malaysiana, 48 (5). pp. 999-1009. ISSN 0126-6039 (P-ISSN) , 2735-0118 (E-ISSN) https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068354475&doi=10.17576%2fjsm-2019-4805-08&partnerID=40&md5=67da582cd7f52591dceaf63432a08c6f http://dx.doi.org/10.17576/jsm-2019-4805-08 http://dx.doi.org/10.17576/jsm-2019-4805-08 |
| spellingShingle | QD1-999 Chemistry QK710-899 Plant physiology Samina Khan Yusufzai Mohammad Shaheen Khan Eunice Lua Hanry Mohd. Rafatullah Belsheera Elison GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis |
| title | GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis |
| title_full | GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis |
| title_fullStr | GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis |
| title_full_unstemmed | GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis |
| title_short | GC-MS analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of Bridelia stipularis |
| title_sort | gc ms analysis of chemical constituents and in vitro antioxidant activity of the organic extracts from the stem of bridelia stipularis |
| topic | QD1-999 Chemistry QK710-899 Plant physiology |
| url | https://eprints.ums.edu.my/id/eprint/30738/1/GC-MS%20analysis%20of%20chemical%20constituents%20and%20in%20vitro%20antioxidant%20activity%20of%20the%20organic%20extracts%20from%20the%20stem%20of%20Bridelia%20stipularis-ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/30738/2/GC-MS%20analysis%20of%20chemical%20constituents%20and%20in%20vitro%20antioxidant%20activity%20of%20the%20organic%20extracts%20from%20the%20stem%20of%20Bridelia%20stipularis.pdf |
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