Comparative transcriptome profiling of horseshoe crab tachypleus gigas hemocytes in response to lipopolysaccharides
Horseshoe crabs (HSCs) are living fossil species of marine arthropods with a long evolutionary history spanning approximately 500 million years. Their survival is helped by their innate immune system that comprises cellular and humoral immune components to protect them against invading pathogens. To...
| Huvudupphovsmän: | , , , , , , , |
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| Materialtyp: | Artikel |
| Språk: | English English |
| Publicerad: |
Elsevier Ltd
2021
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| Ämnen: | |
| Länkar: | https://eprints.ums.edu.my/id/eprint/30884/1/Comparative%20transcriptome%20profiling%20of%20horseshoe%20crab%20tachypleus%20gigas%20hemocytes%20in%20response%20to%20lipopolysaccharides-ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/30884/2/Comparative%20transcriptome%20profiling%20of%20horseshoe%20crab%20Tachypleus%20gigas%20hemocytes%20in%20response%20to%20lipopolysaccharides.pdf |
| _version_ | 1825714422221373440 |
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| author | Maria E. Sarmiento Kai Ling Chin Nyok Sean Lau Ismail Aziah Noraznawati Ismail Mohd Nor Norazmi Armando Acosta Nik Soriani Yaacob |
| author_facet | Maria E. Sarmiento Kai Ling Chin Nyok Sean Lau Ismail Aziah Noraznawati Ismail Mohd Nor Norazmi Armando Acosta Nik Soriani Yaacob |
| author_sort | Maria E. Sarmiento |
| collection | UMS |
| description | Horseshoe crabs (HSCs) are living fossil species of marine arthropods with a long evolutionary history spanning approximately 500 million years. Their survival is helped by their innate immune system that comprises cellular and humoral immune components to protect them against invading pathogens. To help understand the genetic mechanisms involved, the present study utilized the Illumina HiSeq platform to perform transcriptomic analysis of hemocytes from the HSC, Tachypleus gigas, that were challenged with lipopolysaccharides (LPS). The highthroughput sequencing resulted in 352,077,208 and 386,749,136 raw reads corresponding to 282,490,910 and 305,709,830 high-quality mappable reads for the control and LPS-treated hemocyte samples, respectively. Based on the log-fold change of > 0.3 or < -0.3, 1338 genes were significantly upregulated and 215 genes were significantly downregulated following LPS stimulation. The differentially expressed genes (DEGs) were further identified to be associated with multiple pathways such as those related to immune defense, stress response, cytoskeleton function and signal transduction. This study provides insights into the underlying molecular and regulatory mechanisms in hemocytes exposed to LPS, which has relevance for the study of the immune response of HSCs to infection. |
| first_indexed | 2024-03-06T03:11:34Z |
| format | Article |
| id | ums.eprints-30884 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:11:34Z |
| publishDate | 2021 |
| publisher | Elsevier Ltd |
| record_format | dspace |
| spelling | ums.eprints-308842021-10-29T02:37:58Z https://eprints.ums.edu.my/id/eprint/30884/ Comparative transcriptome profiling of horseshoe crab tachypleus gigas hemocytes in response to lipopolysaccharides Maria E. Sarmiento Kai Ling Chin Nyok Sean Lau Ismail Aziah Noraznawati Ismail Mohd Nor Norazmi Armando Acosta Nik Soriani Yaacob QL1-991 Zoology QR1-502 Microbiology Horseshoe crabs (HSCs) are living fossil species of marine arthropods with a long evolutionary history spanning approximately 500 million years. Their survival is helped by their innate immune system that comprises cellular and humoral immune components to protect them against invading pathogens. To help understand the genetic mechanisms involved, the present study utilized the Illumina HiSeq platform to perform transcriptomic analysis of hemocytes from the HSC, Tachypleus gigas, that were challenged with lipopolysaccharides (LPS). The highthroughput sequencing resulted in 352,077,208 and 386,749,136 raw reads corresponding to 282,490,910 and 305,709,830 high-quality mappable reads for the control and LPS-treated hemocyte samples, respectively. Based on the log-fold change of > 0.3 or < -0.3, 1338 genes were significantly upregulated and 215 genes were significantly downregulated following LPS stimulation. The differentially expressed genes (DEGs) were further identified to be associated with multiple pathways such as those related to immune defense, stress response, cytoskeleton function and signal transduction. This study provides insights into the underlying molecular and regulatory mechanisms in hemocytes exposed to LPS, which has relevance for the study of the immune response of HSCs to infection. Elsevier Ltd 2021-08-03 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/30884/1/Comparative%20transcriptome%20profiling%20of%20horseshoe%20crab%20tachypleus%20gigas%20hemocytes%20in%20response%20to%20lipopolysaccharides-ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/30884/2/Comparative%20transcriptome%20profiling%20of%20horseshoe%20crab%20Tachypleus%20gigas%20hemocytes%20in%20response%20to%20lipopolysaccharides.pdf Maria E. Sarmiento and Kai Ling Chin and Nyok Sean Lau and Ismail Aziah and Noraznawati Ismail and Mohd Nor Norazmi and Armando Acosta and Nik Soriani Yaacob (2021) Comparative transcriptome profiling of horseshoe crab tachypleus gigas hemocytes in response to lipopolysaccharides. Fish and Shellfish Immunology, 117. pp. 148-156. ISSN 1050-4648 https://www.sciencedirect.com/science/article/pii/S1050464821002230 https://doi.org/10.1016/j.fsi.2021.08.001 https://doi.org/10.1016/j.fsi.2021.08.001 |
| spellingShingle | QL1-991 Zoology QR1-502 Microbiology Maria E. Sarmiento Kai Ling Chin Nyok Sean Lau Ismail Aziah Noraznawati Ismail Mohd Nor Norazmi Armando Acosta Nik Soriani Yaacob Comparative transcriptome profiling of horseshoe crab tachypleus gigas hemocytes in response to lipopolysaccharides |
| title | Comparative transcriptome profiling of horseshoe crab tachypleus gigas hemocytes in response to lipopolysaccharides |
| title_full | Comparative transcriptome profiling of horseshoe crab tachypleus gigas hemocytes in response to lipopolysaccharides |
| title_fullStr | Comparative transcriptome profiling of horseshoe crab tachypleus gigas hemocytes in response to lipopolysaccharides |
| title_full_unstemmed | Comparative transcriptome profiling of horseshoe crab tachypleus gigas hemocytes in response to lipopolysaccharides |
| title_short | Comparative transcriptome profiling of horseshoe crab tachypleus gigas hemocytes in response to lipopolysaccharides |
| title_sort | comparative transcriptome profiling of horseshoe crab tachypleus gigas hemocytes in response to lipopolysaccharides |
| topic | QL1-991 Zoology QR1-502 Microbiology |
| url | https://eprints.ums.edu.my/id/eprint/30884/1/Comparative%20transcriptome%20profiling%20of%20horseshoe%20crab%20tachypleus%20gigas%20hemocytes%20in%20response%20to%20lipopolysaccharides-ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/30884/2/Comparative%20transcriptome%20profiling%20of%20horseshoe%20crab%20Tachypleus%20gigas%20hemocytes%20in%20response%20to%20lipopolysaccharides.pdf |
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