Snake venom proteomics, immunoreactivity and toxicity neutralization studies for the asiatic mountain pit vipers, ovophis convictus, ovophis tonkinensis, and hime habu, ovophis okinavensis
Snakebite envenomation is a serious neglected tropical disease, and its management is often complicated by the diversity of snake venoms. In Asia, pit vipers of the Ovophis species complex are medically important venomous snakes whose venom properties have not been investigated in depth. This study...
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MDPI
2021
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| Online Access: | https://eprints.ums.edu.my/id/eprint/30908/1/Snake%20Venom%20Proteomics%2C%20Immunoreactivity%20and%20Toxicity%20Neutralization%20Studies%20for%20the%20Asiatic%20Mountain%20Pit%20Vipers-ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/30908/2/Snake%20Venom%20Proteomics%2C%20Immunoreactivity%20and%20Toxicity%20Neutralization%20Studies%20for%20the%20Asiatic%20Mountain%20Pit%20Vipers.pdf |
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| author | Choo Hock Tan Praneetha Palasuberniam Kae Yi Tan |
| author_facet | Choo Hock Tan Praneetha Palasuberniam Kae Yi Tan |
| author_sort | Choo Hock Tan |
| collection | UMS |
| description | Snakebite envenomation is a serious neglected tropical disease, and its management is often complicated by the diversity of snake venoms. In Asia, pit vipers of the Ovophis species complex are medically important venomous snakes whose venom properties have not been investigated in depth. This study characterized the venom proteomes of Ovophis convictus (West Malaysia), Ovophis tonkinensis (northern Vietnam, southern China), and Ovophis okinavensis (Okinawa, Japan) by applying liquid chromatography-tandem mass spectrometry, which detected a high abundance of snake venom serine proteases (SVSP, constituting 40–60% of total venom proteins), followed by phospholipases A2, snake venom metalloproteinases of mainly P-III class, L-amino acid oxidases, and toxins from other protein families which were less abundant. The venoms exhibited different procoagulant activities in human plasma, with potency decreasing from O. tonkinensis > O. okinavensis > O. convictus. The procoagulant nature of venom confirms that consumptive coagulopathy underlies the pathophysiology of Ovophis pit viper envenomation. The hetero-specific antivenoms Gloydius brevicaudus monovalent antivenom (GbMAV) and Trimeresurus albolabris monovalent antivenom (TaMAV) were immunoreactive toward the venoms, and cross-neutralized their procoagulant activities, albeit at variably limited efficacy. In the absence of species-specific antivenom, these hetero-specific antivenoms may be useful in treating coagulotoxic envenomation caused by the different snakes in their respective regions. |
| first_indexed | 2024-03-06T03:11:38Z |
| format | Article |
| id | ums.eprints-30908 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:11:38Z |
| publishDate | 2021 |
| publisher | MDPI |
| record_format | dspace |
| spelling | ums.eprints-309082021-10-29T06:12:41Z https://eprints.ums.edu.my/id/eprint/30908/ Snake venom proteomics, immunoreactivity and toxicity neutralization studies for the asiatic mountain pit vipers, ovophis convictus, ovophis tonkinensis, and hime habu, ovophis okinavensis Choo Hock Tan Praneetha Palasuberniam Kae Yi Tan QL750-795 Animal behavior QP1-(981) Physiology Snakebite envenomation is a serious neglected tropical disease, and its management is often complicated by the diversity of snake venoms. In Asia, pit vipers of the Ovophis species complex are medically important venomous snakes whose venom properties have not been investigated in depth. This study characterized the venom proteomes of Ovophis convictus (West Malaysia), Ovophis tonkinensis (northern Vietnam, southern China), and Ovophis okinavensis (Okinawa, Japan) by applying liquid chromatography-tandem mass spectrometry, which detected a high abundance of snake venom serine proteases (SVSP, constituting 40–60% of total venom proteins), followed by phospholipases A2, snake venom metalloproteinases of mainly P-III class, L-amino acid oxidases, and toxins from other protein families which were less abundant. The venoms exhibited different procoagulant activities in human plasma, with potency decreasing from O. tonkinensis > O. okinavensis > O. convictus. The procoagulant nature of venom confirms that consumptive coagulopathy underlies the pathophysiology of Ovophis pit viper envenomation. The hetero-specific antivenoms Gloydius brevicaudus monovalent antivenom (GbMAV) and Trimeresurus albolabris monovalent antivenom (TaMAV) were immunoreactive toward the venoms, and cross-neutralized their procoagulant activities, albeit at variably limited efficacy. In the absence of species-specific antivenom, these hetero-specific antivenoms may be useful in treating coagulotoxic envenomation caused by the different snakes in their respective regions. MDPI 2021-07-23 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/30908/1/Snake%20Venom%20Proteomics%2C%20Immunoreactivity%20and%20Toxicity%20Neutralization%20Studies%20for%20the%20Asiatic%20Mountain%20Pit%20Vipers-ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/30908/2/Snake%20Venom%20Proteomics%2C%20Immunoreactivity%20and%20Toxicity%20Neutralization%20Studies%20for%20the%20Asiatic%20Mountain%20Pit%20Vipers.pdf Choo Hock Tan and Praneetha Palasuberniam and Kae Yi Tan (2021) Snake venom proteomics, immunoreactivity and toxicity neutralization studies for the asiatic mountain pit vipers, ovophis convictus, ovophis tonkinensis, and hime habu, ovophis okinavensis. Toxins, 13 (517). ISSN 2072-6651 https://www.mdpi.com/2072-6651/13/8/514 https://doi.org/ 10.3390/toxins13080514 https://doi.org/ 10.3390/toxins13080514 |
| spellingShingle | QL750-795 Animal behavior QP1-(981) Physiology Choo Hock Tan Praneetha Palasuberniam Kae Yi Tan Snake venom proteomics, immunoreactivity and toxicity neutralization studies for the asiatic mountain pit vipers, ovophis convictus, ovophis tonkinensis, and hime habu, ovophis okinavensis |
| title | Snake venom proteomics, immunoreactivity and toxicity neutralization studies for the asiatic mountain pit vipers, ovophis convictus, ovophis tonkinensis, and hime habu, ovophis okinavensis |
| title_full | Snake venom proteomics, immunoreactivity and toxicity neutralization studies for the asiatic mountain pit vipers, ovophis convictus, ovophis tonkinensis, and hime habu, ovophis okinavensis |
| title_fullStr | Snake venom proteomics, immunoreactivity and toxicity neutralization studies for the asiatic mountain pit vipers, ovophis convictus, ovophis tonkinensis, and hime habu, ovophis okinavensis |
| title_full_unstemmed | Snake venom proteomics, immunoreactivity and toxicity neutralization studies for the asiatic mountain pit vipers, ovophis convictus, ovophis tonkinensis, and hime habu, ovophis okinavensis |
| title_short | Snake venom proteomics, immunoreactivity and toxicity neutralization studies for the asiatic mountain pit vipers, ovophis convictus, ovophis tonkinensis, and hime habu, ovophis okinavensis |
| title_sort | snake venom proteomics immunoreactivity and toxicity neutralization studies for the asiatic mountain pit vipers ovophis convictus ovophis tonkinensis and hime habu ovophis okinavensis |
| topic | QL750-795 Animal behavior QP1-(981) Physiology |
| url | https://eprints.ums.edu.my/id/eprint/30908/1/Snake%20Venom%20Proteomics%2C%20Immunoreactivity%20and%20Toxicity%20Neutralization%20Studies%20for%20the%20Asiatic%20Mountain%20Pit%20Vipers-ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/30908/2/Snake%20Venom%20Proteomics%2C%20Immunoreactivity%20and%20Toxicity%20Neutralization%20Studies%20for%20the%20Asiatic%20Mountain%20Pit%20Vipers.pdf |
| work_keys_str_mv | AT choohocktan snakevenomproteomicsimmunoreactivityandtoxicityneutralizationstudiesfortheasiaticmountainpitvipersovophisconvictusovophistonkinensisandhimehabuovophisokinavensis AT praneethapalasuberniam snakevenomproteomicsimmunoreactivityandtoxicityneutralizationstudiesfortheasiaticmountainpitvipersovophisconvictusovophistonkinensisandhimehabuovophisokinavensis AT kaeyitan snakevenomproteomicsimmunoreactivityandtoxicityneutralizationstudiesfortheasiaticmountainpitvipersovophisconvictusovophistonkinensisandhimehabuovophisokinavensis |