Preliminary data on the expression profiles of microRNAs in dengue patients infected with DENV-1 serotype
Introduction: MicroRNAs (miRNAs) are highly promising as biomarkers and are an attractive tool for novel therapeutic approaches. The expression of miRNAs in patient’s serum has been broadly used as biomarker candidates against viral infection. Circulatory miRNAs can directly regulate viral genes eit...
| Главные авторы: | , , , , |
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| Формат: | Conference or Workshop Item |
| Язык: | English English |
| Опубликовано: |
2021
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| Online-ссылка: | https://eprints.ums.edu.my/id/eprint/31000/1/Preliminary%20data%20on%20the%20expression%20profiles%20of%20microRNAs%20in%20dengue%20patients%20infected%20with%20DENV-1%20serotype.pdf https://eprints.ums.edu.my/id/eprint/31000/2/Preliminary%20data%20on%20the%20expression%20profiles%20of%20microRNAs%20in%20dengue%20patients%20infected%20with%20DENV-1%20serotype_ABSTRACT.pdf |
| _version_ | 1825714443086987264 |
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| author | Nadia Iryani Najri Vijay Kumar Subbiah Noor Hydayaty Md. Yusuf Rashidah Mohammad Mohammad Zahirul Hoque |
| author_facet | Nadia Iryani Najri Vijay Kumar Subbiah Noor Hydayaty Md. Yusuf Rashidah Mohammad Mohammad Zahirul Hoque |
| author_sort | Nadia Iryani Najri |
| collection | UMS |
| description | Introduction: MicroRNAs (miRNAs) are highly promising as biomarkers and are an attractive tool for novel therapeutic approaches. The expression of miRNAs in patient’s serum has been broadly used as biomarker candidates against viral infection. Circulatory miRNAs can directly regulate viral genes either by promoting or repressing viral replications. Objectives: Thus, we attempted to identify serotype specific miRNA in patients with dengue infection. For the purpose of this paper, we present the expression profile of microRNAs in Dengue Serotype 1 (DENV-1) patients. Methodology: A total of 40 patients with a single DENV-1 serotype infection were identified along with 40 healthy controls. Serum RNAs was isolated from these subjects and subjected to high-throughput small RNA (sRNA) sequencing. This research was approved by the Medical Research Ethics Committee (MREC), Ministry of Health, Malaysia (No. NMRR-18-2782-42195). Result: After, trimming and quality control of the reads, we shortlisted 35 miRNAs candidates that were promising for downstream analysis. From this, 24 miRNAs was upregulated in DENV-1 while, 11 was downregulated in the sera of patients. Conclusion: Differential expression of microRNA may serve as reliable biomarkers of disease severity during early stages of dengue infection. |
| first_indexed | 2024-03-06T03:11:53Z |
| format | Conference or Workshop Item |
| id | ums.eprints-31000 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:11:53Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | ums.eprints-310002021-10-22T01:37:24Z https://eprints.ums.edu.my/id/eprint/31000/ Preliminary data on the expression profiles of microRNAs in dengue patients infected with DENV-1 serotype Nadia Iryani Najri Vijay Kumar Subbiah Noor Hydayaty Md. Yusuf Rashidah Mohammad Mohammad Zahirul Hoque RA643-645 Disease (Communicable and noninfectious) and public health Introduction: MicroRNAs (miRNAs) are highly promising as biomarkers and are an attractive tool for novel therapeutic approaches. The expression of miRNAs in patient’s serum has been broadly used as biomarker candidates against viral infection. Circulatory miRNAs can directly regulate viral genes either by promoting or repressing viral replications. Objectives: Thus, we attempted to identify serotype specific miRNA in patients with dengue infection. For the purpose of this paper, we present the expression profile of microRNAs in Dengue Serotype 1 (DENV-1) patients. Methodology: A total of 40 patients with a single DENV-1 serotype infection were identified along with 40 healthy controls. Serum RNAs was isolated from these subjects and subjected to high-throughput small RNA (sRNA) sequencing. This research was approved by the Medical Research Ethics Committee (MREC), Ministry of Health, Malaysia (No. NMRR-18-2782-42195). Result: After, trimming and quality control of the reads, we shortlisted 35 miRNAs candidates that were promising for downstream analysis. From this, 24 miRNAs was upregulated in DENV-1 while, 11 was downregulated in the sera of patients. Conclusion: Differential expression of microRNA may serve as reliable biomarkers of disease severity during early stages of dengue infection. 2021 Conference or Workshop Item PeerReviewed text en https://eprints.ums.edu.my/id/eprint/31000/1/Preliminary%20data%20on%20the%20expression%20profiles%20of%20microRNAs%20in%20dengue%20patients%20infected%20with%20DENV-1%20serotype.pdf text en https://eprints.ums.edu.my/id/eprint/31000/2/Preliminary%20data%20on%20the%20expression%20profiles%20of%20microRNAs%20in%20dengue%20patients%20infected%20with%20DENV-1%20serotype_ABSTRACT.pdf Nadia Iryani Najri and Vijay Kumar Subbiah and Noor Hydayaty Md. Yusuf and Rashidah Mohammad and Mohammad Zahirul Hoque (2021) Preliminary data on the expression profiles of microRNAs in dengue patients infected with DENV-1 serotype. In: 14th Malaysia International Genetic Congress (MIGC14), 15 -17 March 2021, Malaysia (Virtual Conference). https://migc-pgm.com/images/saintifik/program_book_MiGC14.pdf |
| spellingShingle | RA643-645 Disease (Communicable and noninfectious) and public health Nadia Iryani Najri Vijay Kumar Subbiah Noor Hydayaty Md. Yusuf Rashidah Mohammad Mohammad Zahirul Hoque Preliminary data on the expression profiles of microRNAs in dengue patients infected with DENV-1 serotype |
| title | Preliminary data on the expression profiles of microRNAs in dengue patients infected with DENV-1 serotype |
| title_full | Preliminary data on the expression profiles of microRNAs in dengue patients infected with DENV-1 serotype |
| title_fullStr | Preliminary data on the expression profiles of microRNAs in dengue patients infected with DENV-1 serotype |
| title_full_unstemmed | Preliminary data on the expression profiles of microRNAs in dengue patients infected with DENV-1 serotype |
| title_short | Preliminary data on the expression profiles of microRNAs in dengue patients infected with DENV-1 serotype |
| title_sort | preliminary data on the expression profiles of micrornas in dengue patients infected with denv 1 serotype |
| topic | RA643-645 Disease (Communicable and noninfectious) and public health |
| url | https://eprints.ums.edu.my/id/eprint/31000/1/Preliminary%20data%20on%20the%20expression%20profiles%20of%20microRNAs%20in%20dengue%20patients%20infected%20with%20DENV-1%20serotype.pdf https://eprints.ums.edu.my/id/eprint/31000/2/Preliminary%20data%20on%20the%20expression%20profiles%20of%20microRNAs%20in%20dengue%20patients%20infected%20with%20DENV-1%20serotype_ABSTRACT.pdf |
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