Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken
An accurate and sensitive liquid chromatography-electrospray ionisation/multi stage mass spectrometry (LC–ESI–MS/MS) method has been developed for the characterization and quantitation of nine heterocyclic amine (HCA) compounds. We were identified 2-Amino-3-methyl-3H-imidazo[4,5-F]quinoline (IQ), 2-...
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| Format: | Article |
| Language: | English |
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Springer Science + Business Media
2020
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| Online Access: | https://eprints.ums.edu.my/id/eprint/31155/1/Effect%20of%20a%20different%20mobile%20phase%20on%20LC_ESI_MS_MS%20performance%20for%20the%20identification%20and%20quantitation%20of%20polar%20and%20nonpolar%20heterocyclic%20amines%20in%20cooked%20chicken_ABSTRACT.pdf |
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| author | Jinap, S. Jaafar, S.N. Hasnol, N.D.S. Md Jahurul Haque Akanda |
| author_facet | Jinap, S. Jaafar, S.N. Hasnol, N.D.S. Md Jahurul Haque Akanda |
| author_sort | Jinap, S. |
| collection | UMS |
| description | An accurate and sensitive liquid chromatography-electrospray ionisation/multi stage mass spectrometry (LC–ESI–MS/MS) method has been developed for the characterization and quantitation of nine heterocyclic amine (HCA) compounds. We were identified 2-Amino-3-methyl-3H-imidazo[4,5-F]quinoline (IQ), 2-Amino-3-methyl-3H-imidazo[4,5-f]quinoxaline (IQx), 2-Amino-3,4-dimethyl-3H-imidazo[4,5-f]quinoline (MeIQ), 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-Amino-3,7,8-trimethyl-3H-imidazo[4,5-f]quinoxaline (7,8-DiMeIQx), 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine as polar molecules, and they were eluted accordingly at the beginning of the analysis. However, Norharman, Harman and AαC were identified as a non-polar molecules and were eluted last. The excellent selectivity and sensitivity achieved by the selected reaction monitoring mode permitted satisfactory quantitation and confirmation of the injected HCA compounds with limit of detection and quantitation ranges from 0.17 to 1.44 pg and 0.53 to 4.57 pg, respectively. The recoveries ranged from 74 to 108%, with an interday and intraday precision of 1.6–1.9% and 0.1–0.9%, respectively. The method was successfully applied to identify and quantify the studied HCAs in fried and grilled chicken. |
| first_indexed | 2024-03-06T03:12:15Z |
| format | Article |
| id | ums.eprints-31155 |
| institution | Universiti Malaysia Sabah |
| language | English |
| last_indexed | 2024-03-06T03:12:15Z |
| publishDate | 2020 |
| publisher | Springer Science + Business Media |
| record_format | dspace |
| spelling | ums.eprints-311552021-11-23T02:15:27Z https://eprints.ums.edu.my/id/eprint/31155/ Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken Jinap, S. Jaafar, S.N. Hasnol, N.D.S. Md Jahurul Haque Akanda QP501-801 Animal biochemistry An accurate and sensitive liquid chromatography-electrospray ionisation/multi stage mass spectrometry (LC–ESI–MS/MS) method has been developed for the characterization and quantitation of nine heterocyclic amine (HCA) compounds. We were identified 2-Amino-3-methyl-3H-imidazo[4,5-F]quinoline (IQ), 2-Amino-3-methyl-3H-imidazo[4,5-f]quinoxaline (IQx), 2-Amino-3,4-dimethyl-3H-imidazo[4,5-f]quinoline (MeIQ), 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-Amino-3,7,8-trimethyl-3H-imidazo[4,5-f]quinoxaline (7,8-DiMeIQx), 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine as polar molecules, and they were eluted accordingly at the beginning of the analysis. However, Norharman, Harman and AαC were identified as a non-polar molecules and were eluted last. The excellent selectivity and sensitivity achieved by the selected reaction monitoring mode permitted satisfactory quantitation and confirmation of the injected HCA compounds with limit of detection and quantitation ranges from 0.17 to 1.44 pg and 0.53 to 4.57 pg, respectively. The recoveries ranged from 74 to 108%, with an interday and intraday precision of 1.6–1.9% and 0.1–0.9%, respectively. The method was successfully applied to identify and quantify the studied HCAs in fried and grilled chicken. Springer Science + Business Media 2020 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/31155/1/Effect%20of%20a%20different%20mobile%20phase%20on%20LC_ESI_MS_MS%20performance%20for%20the%20identification%20and%20quantitation%20of%20polar%20and%20nonpolar%20heterocyclic%20amines%20in%20cooked%20chicken_ABSTRACT.pdf Jinap, S. and Jaafar, S.N. and Hasnol, N.D.S. and Md Jahurul Haque Akanda (2020) Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken. Journal of Food Measurement and Characterization, 14. pp. 262-271. ISSN 2193-4126 (P-ISSN) , 2193-4134 (E-ISSN) https://pubag.nal.usda.gov/catalog/6843721 https://doi.org/10.1007/s11694-019-00288-0 https://doi.org/10.1007/s11694-019-00288-0 |
| spellingShingle | QP501-801 Animal biochemistry Jinap, S. Jaafar, S.N. Hasnol, N.D.S. Md Jahurul Haque Akanda Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| title | Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| title_full | Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| title_fullStr | Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| title_full_unstemmed | Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| title_short | Effect of a different mobile phase on LC–ESI–MS/MS performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| title_sort | effect of a different mobile phase on lc esi ms ms performance for the identification and quantitation of polar and nonpolar heterocyclic amines in cooked chicken |
| topic | QP501-801 Animal biochemistry |
| url | https://eprints.ums.edu.my/id/eprint/31155/1/Effect%20of%20a%20different%20mobile%20phase%20on%20LC_ESI_MS_MS%20performance%20for%20the%20identification%20and%20quantitation%20of%20polar%20and%20nonpolar%20heterocyclic%20amines%20in%20cooked%20chicken_ABSTRACT.pdf |
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