Peptide conjugate on multilayer graphene oxide film for the osteogenic differentiation of human Wharton’s jelly-derived mesenchymal stem cells
Graphene oxide (GO) is extensively studied as a template material for mesenchymal stem cell application due to its two-dimensional nature and unique functionalization chemistries. Herein, a new type of peptide-conjugated multilayer graphene oxide (peptide/m-GO film) was fabricated and used as biomat...
| _version_ | 1825714528227164160 |
|---|---|
| author | Perng Yang Puah Pak, Yan Moh Coswald Stephen Sipaut @ Mohd Nasri Ping, Chin Lee Siew, Eng How |
| author_facet | Perng Yang Puah Pak, Yan Moh Coswald Stephen Sipaut @ Mohd Nasri Ping, Chin Lee Siew, Eng How |
| author_sort | Perng Yang Puah |
| collection | UMS |
| description | Graphene oxide (GO) is extensively studied as a template material for mesenchymal stem cell application due to its two-dimensional nature and unique functionalization chemistries. Herein, a new type of peptide-conjugated multilayer graphene oxide (peptide/m-GO film) was fabricated and used as biomaterial for culturing human Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs). The characterization of the peptide/m-GO films was performed, and the biocompatibility of the WJ-MSCs on the peptide/m-GO films was investigated. The results demonstrated that the peptide conjugate on the m-GO film did not hamper the normal growth of WJ-MSCs but supported the growth of WJ-MSCs after the 6-day culture period. In addition, the osteogenic differentiation of WJMSCs on the peptide/m-GO films was enhanced as compared with the parent m-GO film. Therefore, such peptide-conjugated m-GO films could provide a highly biocompatible and multifunctional 2D material to tailor the potential application of WJ-MSCs in bone tissue regeneration. |
| first_indexed | 2024-03-06T03:13:10Z |
| format | Article |
| id | ums.eprints-31529 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:13:10Z |
| publishDate | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
| record_format | dspace |
| spelling | ums.eprints-315292021-12-22T09:56:15Z https://eprints.ums.edu.my/id/eprint/31529/ Peptide conjugate on multilayer graphene oxide film for the osteogenic differentiation of human Wharton’s jelly-derived mesenchymal stem cells Perng Yang Puah Pak, Yan Moh Coswald Stephen Sipaut @ Mohd Nasri Ping, Chin Lee Siew, Eng How Q1-295 General QD1-999 Chemistry Graphene oxide (GO) is extensively studied as a template material for mesenchymal stem cell application due to its two-dimensional nature and unique functionalization chemistries. Herein, a new type of peptide-conjugated multilayer graphene oxide (peptide/m-GO film) was fabricated and used as biomaterial for culturing human Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs). The characterization of the peptide/m-GO films was performed, and the biocompatibility of the WJ-MSCs on the peptide/m-GO films was investigated. The results demonstrated that the peptide conjugate on the m-GO film did not hamper the normal growth of WJ-MSCs but supported the growth of WJ-MSCs after the 6-day culture period. In addition, the osteogenic differentiation of WJMSCs on the peptide/m-GO films was enhanced as compared with the parent m-GO film. Therefore, such peptide-conjugated m-GO films could provide a highly biocompatible and multifunctional 2D material to tailor the potential application of WJ-MSCs in bone tissue regeneration. Multidisciplinary Digital Publishing Institute (MDPI) 2021 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/31529/1/Peptide%20conjugate%20on%20multilayer%20graphene%20oxide%20film%20for%20the%20osteogenic%20differentiation%20of%20human%20Wharton%E2%80%99s%20jelly-derived%20mesenchymal%20stem%20cells.pdf text en https://eprints.ums.edu.my/id/eprint/31529/2/Peptide%20conjugate%20on%20multilayer%20graphene%20oxide%20film%20for%20the%20osteogenic%20differentiation%20of%20human%20Wharton%E2%80%99s%20jelly-derived%20mesenchymal%20stem%20cells1.pdf Perng Yang Puah and Pak, Yan Moh and Coswald Stephen Sipaut @ Mohd Nasri and Ping, Chin Lee and Siew, Eng How (2021) Peptide conjugate on multilayer graphene oxide film for the osteogenic differentiation of human Wharton’s jelly-derived mesenchymal stem cells. Polymers, 13 (3290). pp. 1-13. ISSN 2073-4360 https://www.mdpi.com/2073-4360/13/19/3290/htm https://doi.org/10.3390/polym13193290 https://doi.org/10.3390/polym13193290 |
| spellingShingle | Q1-295 General QD1-999 Chemistry Perng Yang Puah Pak, Yan Moh Coswald Stephen Sipaut @ Mohd Nasri Ping, Chin Lee Siew, Eng How Peptide conjugate on multilayer graphene oxide film for the osteogenic differentiation of human Wharton’s jelly-derived mesenchymal stem cells |
| title | Peptide conjugate on multilayer graphene oxide film for the osteogenic differentiation of human Wharton’s jelly-derived mesenchymal stem cells |
| title_full | Peptide conjugate on multilayer graphene oxide film for the osteogenic differentiation of human Wharton’s jelly-derived mesenchymal stem cells |
| title_fullStr | Peptide conjugate on multilayer graphene oxide film for the osteogenic differentiation of human Wharton’s jelly-derived mesenchymal stem cells |
| title_full_unstemmed | Peptide conjugate on multilayer graphene oxide film for the osteogenic differentiation of human Wharton’s jelly-derived mesenchymal stem cells |
| title_short | Peptide conjugate on multilayer graphene oxide film for the osteogenic differentiation of human Wharton’s jelly-derived mesenchymal stem cells |
| title_sort | peptide conjugate on multilayer graphene oxide film for the osteogenic differentiation of human wharton s jelly derived mesenchymal stem cells |
| topic | Q1-295 General QD1-999 Chemistry |
| url | https://eprints.ums.edu.my/id/eprint/31529/1/Peptide%20conjugate%20on%20multilayer%20graphene%20oxide%20film%20for%20the%20osteogenic%20differentiation%20of%20human%20Wharton%E2%80%99s%20jelly-derived%20mesenchymal%20stem%20cells.pdf https://eprints.ums.edu.my/id/eprint/31529/2/Peptide%20conjugate%20on%20multilayer%20graphene%20oxide%20film%20for%20the%20osteogenic%20differentiation%20of%20human%20Wharton%E2%80%99s%20jelly-derived%20mesenchymal%20stem%20cells1.pdf |
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