Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia
A simple, ready-to-use concentrated specimen smear microscopy method employing a nanometer silicon polyvinylidene fluoride (PVDF) polymer membrane sandwich filtration vessel to concentrate acid-fast bacilli (AFB) in samples (SFV-CSSM, Hunan-Tech New Medical System Co. Ltd. China) was compared with d...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English English |
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Elsevier Ltd.
2022
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| Online Access: | https://eprints.ums.edu.my/id/eprint/31876/1/Concentrated%20specimen%20smear%20microscopy%20utilising%20a%20polymer%20membrane%20.ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/31876/2/Concentrated%20specimen%20smear%20microscopy%20utilising%20a%20polymer%20membrane.pdf |
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| author | Dawn Carmel Paul Yun Fong Ngeow Sook Fan Yap Jiloris F. Dony Richard Avoi Rashidah Mohammad Hien Fuh Ng |
| author_facet | Dawn Carmel Paul Yun Fong Ngeow Sook Fan Yap Jiloris F. Dony Richard Avoi Rashidah Mohammad Hien Fuh Ng |
| author_sort | Dawn Carmel Paul |
| collection | UMS |
| description | A simple, ready-to-use concentrated specimen smear microscopy method employing a nanometer silicon polyvinylidene fluoride (PVDF) polymer membrane sandwich filtration vessel to concentrate acid-fast bacilli (AFB) in samples (SFV-CSSM, Hunan-Tech New Medical System Co. Ltd. China) was compared with direct sputum smear microscopy (DSSM) to determine its performance using culture on modified Ogawa agar as reference. The results for 4114 clinical samples collected from health facilities in Sabah were interpreted with reference to culture results, sample collection-transportation conditions and clinical data including responses to anti-TB drug treatment. The SFV-CSSM showed higher sensitivity than DSSM (79.4% versus 60.5%) and less background interference. Its ability to detect low levels of AFB at an affordable cost makes it an excellent tool for the screening of pauci-bacillary samples as well as for active case finding in TB control programs. |
| first_indexed | 2024-03-06T03:14:04Z |
| format | Article |
| id | ums.eprints-31876 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:14:04Z |
| publishDate | 2022 |
| publisher | Elsevier Ltd. |
| record_format | dspace |
| spelling | ums.eprints-318762022-03-24T07:21:37Z https://eprints.ums.edu.my/id/eprint/31876/ Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia Dawn Carmel Paul Yun Fong Ngeow Sook Fan Yap Jiloris F. Dony Richard Avoi Rashidah Mohammad Hien Fuh Ng RA1-1270 Public aspects of medicine A simple, ready-to-use concentrated specimen smear microscopy method employing a nanometer silicon polyvinylidene fluoride (PVDF) polymer membrane sandwich filtration vessel to concentrate acid-fast bacilli (AFB) in samples (SFV-CSSM, Hunan-Tech New Medical System Co. Ltd. China) was compared with direct sputum smear microscopy (DSSM) to determine its performance using culture on modified Ogawa agar as reference. The results for 4114 clinical samples collected from health facilities in Sabah were interpreted with reference to culture results, sample collection-transportation conditions and clinical data including responses to anti-TB drug treatment. The SFV-CSSM showed higher sensitivity than DSSM (79.4% versus 60.5%) and less background interference. Its ability to detect low levels of AFB at an affordable cost makes it an excellent tool for the screening of pauci-bacillary samples as well as for active case finding in TB control programs. Elsevier Ltd. 2022-02-13 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/31876/1/Concentrated%20specimen%20smear%20microscopy%20utilising%20a%20polymer%20membrane%20.ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/31876/2/Concentrated%20specimen%20smear%20microscopy%20utilising%20a%20polymer%20membrane.pdf Dawn Carmel Paul and Yun Fong Ngeow and Sook Fan Yap and Jiloris F. Dony and Richard Avoi and Rashidah Mohammad and Hien Fuh Ng (2022) Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia. Tuberculosis, 133. pp. 1-6. ISSN 1472-9792 https://www.sciencedirect.com/science/article/abs/pii/S1472979222000208 https://doi.org/10.1016/j.tube.2022.102183 https://doi.org/10.1016/j.tube.2022.102183 |
| spellingShingle | RA1-1270 Public aspects of medicine Dawn Carmel Paul Yun Fong Ngeow Sook Fan Yap Jiloris F. Dony Richard Avoi Rashidah Mohammad Hien Fuh Ng Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia |
| title | Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia |
| title_full | Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia |
| title_fullStr | Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia |
| title_full_unstemmed | Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia |
| title_short | Concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid-fast bacilli in health facilities in Sabah, East Malaysia |
| title_sort | concentrated specimen smear microscopy utilising a polymer membrane sandwich filtration vessel for the detection of acid fast bacilli in health facilities in sabah east malaysia |
| topic | RA1-1270 Public aspects of medicine |
| url | https://eprints.ums.edu.my/id/eprint/31876/1/Concentrated%20specimen%20smear%20microscopy%20utilising%20a%20polymer%20membrane%20.ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/31876/2/Concentrated%20specimen%20smear%20microscopy%20utilising%20a%20polymer%20membrane.pdf |
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