Callus formation and plant regeneration from immature embryos of pigmented upland rice (Oryza sativa cv. Tadong)
The application of biotechnology in upland rice improvement programs depends on the availability of efficient regeneration protocols. Although protocols for shoot regeneration of upland rice are available, none has been reported for pigmented cultivars. This study reports on a protocol for callus in...
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| Format: | Article |
| Language: | English English |
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Penerbit UTM
2021
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| Online Access: | https://eprints.ums.edu.my/id/eprint/31989/3/Callus%20Formation%20and%20Plant%20Regeneration%20from%20Immature%20Embryos%20of%20Pigmented%20Upland%20Rice%20%28Oryza%20sativa%20cv.%20Tadong%29_ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/31989/1/Callus%20Formation%20and%20Plant%20Regeneration%20from%20Immature%20Embryos%20of%20Pigmented%20Upland%20Rice%20%28Oryza%20sativa%20cv.%20Tadong%29.pdf |
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| author | Hoo, Kah Yan Lee, Ping Chin Mariam A. Latip Loumi @ Noumie Surugau Zaleha A. Aziz |
| author_facet | Hoo, Kah Yan Lee, Ping Chin Mariam A. Latip Loumi @ Noumie Surugau Zaleha A. Aziz |
| author_sort | Hoo, Kah Yan |
| collection | UMS |
| description | The application of biotechnology in upland rice improvement programs depends on the availability of efficient regeneration protocols. Although protocols for shoot regeneration of upland rice are available, none has been reported for pigmented cultivars. This study reports on a protocol for callus induction and regeneration of Tadong, a pigmented upland rice cultivar from Sabah. For callus induction, immature embryos were cultured on media containing 2,4-Dichlorophenoxyacetic (2,4-D) at various concentrations (0 – 2.5 mg/L) and on different types of media (MS; MSB5; N6B5; N6). To induce shoot regeneration, callus explants were cultured on MS medium supplemented with combinations of 6-Benzylaminopurine (BAP) at various concentrations (0 – 3.0 mg/L) and 1-Naphthaleneacetic acid (NAA) at 1.0 mg/L. To induce shoot development, callus explants were pre-treated with Thidiazuron (TDZ) at various concentrations (0-1.0 mg/l) and exposed to different desiccation periods (0 – 72 hours). 2,4-Dichlorophenoxyacetic at 2.5 mg/L and N6B5 medium resulted in the highest percentages of explant forming callus which were 60.3 ± 17.0 % and 58.7 ± 9.8 % respectively. The regeneration media failed to induce shoot on callus explants, instead, green spots were formed on the surface of the callus. The green spots were stimulated to develop into shoots when the callus explants were pre-treated with 0.5 mg/L TDZ or exposed to partial desiccation for 24 h, the percentages of explant forming shoot were 35.7 ± 4.8 % and 47.7 ± 6.8 % respectively. Shoots developed into complete plants on hormone-free MS medium and acclimatized. |
| first_indexed | 2024-03-06T03:14:22Z |
| format | Article |
| id | ums.eprints-31989 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:14:22Z |
| publishDate | 2021 |
| publisher | Penerbit UTM |
| record_format | dspace |
| spelling | ums.eprints-319892022-03-22T07:06:30Z https://eprints.ums.edu.my/id/eprint/31989/ Callus formation and plant regeneration from immature embryos of pigmented upland rice (Oryza sativa cv. Tadong) Hoo, Kah Yan Lee, Ping Chin Mariam A. Latip Loumi @ Noumie Surugau Zaleha A. Aziz SB113.2-118.46 Seeds. Seed technology The application of biotechnology in upland rice improvement programs depends on the availability of efficient regeneration protocols. Although protocols for shoot regeneration of upland rice are available, none has been reported for pigmented cultivars. This study reports on a protocol for callus induction and regeneration of Tadong, a pigmented upland rice cultivar from Sabah. For callus induction, immature embryos were cultured on media containing 2,4-Dichlorophenoxyacetic (2,4-D) at various concentrations (0 – 2.5 mg/L) and on different types of media (MS; MSB5; N6B5; N6). To induce shoot regeneration, callus explants were cultured on MS medium supplemented with combinations of 6-Benzylaminopurine (BAP) at various concentrations (0 – 3.0 mg/L) and 1-Naphthaleneacetic acid (NAA) at 1.0 mg/L. To induce shoot development, callus explants were pre-treated with Thidiazuron (TDZ) at various concentrations (0-1.0 mg/l) and exposed to different desiccation periods (0 – 72 hours). 2,4-Dichlorophenoxyacetic at 2.5 mg/L and N6B5 medium resulted in the highest percentages of explant forming callus which were 60.3 ± 17.0 % and 58.7 ± 9.8 % respectively. The regeneration media failed to induce shoot on callus explants, instead, green spots were formed on the surface of the callus. The green spots were stimulated to develop into shoots when the callus explants were pre-treated with 0.5 mg/L TDZ or exposed to partial desiccation for 24 h, the percentages of explant forming shoot were 35.7 ± 4.8 % and 47.7 ± 6.8 % respectively. Shoots developed into complete plants on hormone-free MS medium and acclimatized. Penerbit UTM 2021 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/31989/3/Callus%20Formation%20and%20Plant%20Regeneration%20from%20Immature%20Embryos%20of%20Pigmented%20Upland%20Rice%20%28Oryza%20sativa%20cv.%20Tadong%29_ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/31989/1/Callus%20Formation%20and%20Plant%20Regeneration%20from%20Immature%20Embryos%20of%20Pigmented%20Upland%20Rice%20%28Oryza%20sativa%20cv.%20Tadong%29.pdf Hoo, Kah Yan and Lee, Ping Chin and Mariam A. Latip and Loumi @ Noumie Surugau and Zaleha A. Aziz (2021) Callus formation and plant regeneration from immature embryos of pigmented upland rice (Oryza sativa cv. Tadong). Jurnal Teknologi (Sciences and Engineering), 83. pp. 91-100. ISSN 2180-3722 https://journals.utm.my/jurnalteknologi/article/view/14518/7694 https://doi.org/10.11113/jurnalteknologi.v83.14518%7C https://doi.org/10.11113/jurnalteknologi.v83.14518%7C |
| spellingShingle | SB113.2-118.46 Seeds. Seed technology Hoo, Kah Yan Lee, Ping Chin Mariam A. Latip Loumi @ Noumie Surugau Zaleha A. Aziz Callus formation and plant regeneration from immature embryos of pigmented upland rice (Oryza sativa cv. Tadong) |
| title | Callus formation and plant regeneration from immature embryos of pigmented upland rice (Oryza sativa cv. Tadong) |
| title_full | Callus formation and plant regeneration from immature embryos of pigmented upland rice (Oryza sativa cv. Tadong) |
| title_fullStr | Callus formation and plant regeneration from immature embryos of pigmented upland rice (Oryza sativa cv. Tadong) |
| title_full_unstemmed | Callus formation and plant regeneration from immature embryos of pigmented upland rice (Oryza sativa cv. Tadong) |
| title_short | Callus formation and plant regeneration from immature embryos of pigmented upland rice (Oryza sativa cv. Tadong) |
| title_sort | callus formation and plant regeneration from immature embryos of pigmented upland rice oryza sativa cv tadong |
| topic | SB113.2-118.46 Seeds. Seed technology |
| url | https://eprints.ums.edu.my/id/eprint/31989/3/Callus%20Formation%20and%20Plant%20Regeneration%20from%20Immature%20Embryos%20of%20Pigmented%20Upland%20Rice%20%28Oryza%20sativa%20cv.%20Tadong%29_ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/31989/1/Callus%20Formation%20and%20Plant%20Regeneration%20from%20Immature%20Embryos%20of%20Pigmented%20Upland%20Rice%20%28Oryza%20sativa%20cv.%20Tadong%29.pdf |
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