Antioxidant screening of Garcinia forbesii originated from Sabah
Garcinia forbesii is a wild-type of plant that have been long used traditionally with broad utilities in several fields like medicines, cosmetics, food and neutraceutics. Increasing awareness towards the use of phytochemicals and other plant derives products worldwide has broaden the study of bioact...
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| Format: | Proceedings |
| Language: | English English |
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Faculty of Science and Natural Resources
2021
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| Online Access: | https://eprints.ums.edu.my/id/eprint/32031/5/Antioxidant%20screening%20of%20Garcinia%20forbesii%20originated%20from%20Sabah.ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/32031/2/ANTIOXIDANT%20SCREENING%20OF%20GARCINIA%20FORBESII%20ORIGINATED%20FROM%20SABAH.pdf |
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| author | Suraya Abdul Sani Mohammad Khalizan Sabullah Wan Fahmi Wan Mohamad Nazarie Ainol Azifa Mohd Faik Rahmath Abdulla |
| author_facet | Suraya Abdul Sani Mohammad Khalizan Sabullah Wan Fahmi Wan Mohamad Nazarie Ainol Azifa Mohd Faik Rahmath Abdulla |
| author_sort | Suraya Abdul Sani |
| collection | UMS |
| description | Garcinia forbesii is a wild-type of plant that have been long used traditionally with broad utilities in several fields like medicines, cosmetics, food and neutraceutics. Increasing awareness towards the use of phytochemicals and other plant derives products worldwide has broaden the study of bioactivities from several industrial sectors. Therefore, the present study aims to screen the antioxidant and antimicrobial properties of fruits and leaves of Garcinia forbesii. The methanolic, hexanic and ethyl acetate extracts were obtained by maceration extraction, isolated and undergoes purification through Thin layer Chromatography (TLC) and Column Chromatography (CC). The highest yield of extraction are both from methanol extracts of fruits and leaves which are 9.26 ± 0.34 g and 7.04 ±0.21 g. The antimicrobial activity of the extracts was determined against Escherichia coli using Kirby-Bauer method. The disc diffusion assay showed that the inhibition of growth of E. coli was fully attributed to hexanic extract for fruits and leaves of G. forbesii while all other extracts displayed minimum or none inhibition zone. The inhibition zone of hexanic extract of leaves (11.83mm ± 1.04) showed the highest than fruits (9.33mm ± 1.53) among all the fractions. Antioxidant activities of the leave extracts of G. forbesii in reducing power, FRAP (ferric reducing antioxidant power) and DPPH followed the same order of methanolic > ethyl acetate > hexanic. Meanwhile, extract hexane, methanol and ethyl acetate of the fruits of G. forbesii showed IC50 values at 1.05%, 3.35% and 4.44% while for leaves are at 1.19%, 2.4% and 8.94% respectively. Methanol is therefore a better solvent to extract most of the antioxidant components from G. forbesii leaves. |
| first_indexed | 2024-03-06T03:14:29Z |
| format | Proceedings |
| id | ums.eprints-32031 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:14:29Z |
| publishDate | 2021 |
| publisher | Faculty of Science and Natural Resources |
| record_format | dspace |
| spelling | ums.eprints-320312022-03-30T09:41:12Z https://eprints.ums.edu.my/id/eprint/32031/ Antioxidant screening of Garcinia forbesii originated from Sabah Suraya Abdul Sani Mohammad Khalizan Sabullah Wan Fahmi Wan Mohamad Nazarie Ainol Azifa Mohd Faik Rahmath Abdulla QK710-899 Plant physiology Garcinia forbesii is a wild-type of plant that have been long used traditionally with broad utilities in several fields like medicines, cosmetics, food and neutraceutics. Increasing awareness towards the use of phytochemicals and other plant derives products worldwide has broaden the study of bioactivities from several industrial sectors. Therefore, the present study aims to screen the antioxidant and antimicrobial properties of fruits and leaves of Garcinia forbesii. The methanolic, hexanic and ethyl acetate extracts were obtained by maceration extraction, isolated and undergoes purification through Thin layer Chromatography (TLC) and Column Chromatography (CC). The highest yield of extraction are both from methanol extracts of fruits and leaves which are 9.26 ± 0.34 g and 7.04 ±0.21 g. The antimicrobial activity of the extracts was determined against Escherichia coli using Kirby-Bauer method. The disc diffusion assay showed that the inhibition of growth of E. coli was fully attributed to hexanic extract for fruits and leaves of G. forbesii while all other extracts displayed minimum or none inhibition zone. The inhibition zone of hexanic extract of leaves (11.83mm ± 1.04) showed the highest than fruits (9.33mm ± 1.53) among all the fractions. Antioxidant activities of the leave extracts of G. forbesii in reducing power, FRAP (ferric reducing antioxidant power) and DPPH followed the same order of methanolic > ethyl acetate > hexanic. Meanwhile, extract hexane, methanol and ethyl acetate of the fruits of G. forbesii showed IC50 values at 1.05%, 3.35% and 4.44% while for leaves are at 1.19%, 2.4% and 8.94% respectively. Methanol is therefore a better solvent to extract most of the antioxidant components from G. forbesii leaves. Faculty of Science and Natural Resources 2021-10 Proceedings PeerReviewed text en https://eprints.ums.edu.my/id/eprint/32031/5/Antioxidant%20screening%20of%20Garcinia%20forbesii%20originated%20from%20Sabah.ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/32031/2/ANTIOXIDANT%20SCREENING%20OF%20GARCINIA%20FORBESII%20ORIGINATED%20FROM%20SABAH.pdf Suraya Abdul Sani and Mohammad Khalizan Sabullah and Wan Fahmi Wan Mohamad Nazarie and Ainol Azifa Mohd Faik and Rahmath Abdulla (2021) Antioxidant screening of Garcinia forbesii originated from Sabah. https://drive.google.com/file/d/1zKEpSiYvg9vZHHzuKra49512D_AMLubu/view |
| spellingShingle | QK710-899 Plant physiology Suraya Abdul Sani Mohammad Khalizan Sabullah Wan Fahmi Wan Mohamad Nazarie Ainol Azifa Mohd Faik Rahmath Abdulla Antioxidant screening of Garcinia forbesii originated from Sabah |
| title | Antioxidant screening of Garcinia forbesii originated from Sabah |
| title_full | Antioxidant screening of Garcinia forbesii originated from Sabah |
| title_fullStr | Antioxidant screening of Garcinia forbesii originated from Sabah |
| title_full_unstemmed | Antioxidant screening of Garcinia forbesii originated from Sabah |
| title_short | Antioxidant screening of Garcinia forbesii originated from Sabah |
| title_sort | antioxidant screening of garcinia forbesii originated from sabah |
| topic | QK710-899 Plant physiology |
| url | https://eprints.ums.edu.my/id/eprint/32031/5/Antioxidant%20screening%20of%20Garcinia%20forbesii%20originated%20from%20Sabah.ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/32031/2/ANTIOXIDANT%20SCREENING%20OF%20GARCINIA%20FORBESII%20ORIGINATED%20FROM%20SABAH.pdf |
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