Growth and lipid production of isochrysis galbana in an upscale cultivation system

Microalgae for biofuel production require further research and development to be economically viable, especially in terms of cost and biomass production. These include the need to optimize the favorable growth conditions for low-cost but large-scale cultivation. This study aims to determine the best...

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Main Authors: Erma Rizah Abdul Sani, Thau, Wilson Lym Yong, Chin, Grace Joy Wei Lie, Mailin Misson
Format: Article
Language:English
English
Published: UniSE Press 2021
Subjects:
Online Access:https://eprints.ums.edu.my/id/eprint/32050/1/Growth%20and%20lipid%20production%20of%20isochrysis%20galbana%20in%20an%20upscale%20cultivation%20system_ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/32050/2/Growth%20and%20lipid%20production%20of%20isochrysis%20galbana%20in%20an%20upscale%20cultivation%20system.pdf
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author Erma Rizah Abdul Sani
Thau, Wilson Lym Yong
Chin, Grace Joy Wei Lie
Mailin Misson
author_facet Erma Rizah Abdul Sani
Thau, Wilson Lym Yong
Chin, Grace Joy Wei Lie
Mailin Misson
author_sort Erma Rizah Abdul Sani
collection UMS
description Microalgae for biofuel production require further research and development to be economically viable, especially in terms of cost and biomass production. These include the need to optimize the favorable growth conditions for low-cost but large-scale cultivation. This study aims to determine the best initial biomass concentration of Isochrysis galbana on a pilot scale cultivation system. Isochrysis galbana was cultured for 69 days in an upscaled 2-liter Erlemenmeyer flask with a variety of initial biomass concentrations using the previously established stock culture (250 ml) at 25°C room temperature, 16:8 light/dark cycle, and 135 µmol/m2/s light intensity. The initial biomass concentration was optimized from a range of 103 cells/ml to 104 cells/ml, 105 cells/ml, and 106 cells/ml. The cell density was calculated every three days to determine the growth curve, and the lipid content was measured weekly throughout the cultivation cycle. The results show that the 106 cells/ml initial concentrations produced the highest growth, but the 104 cells/ml initial concentration produced the highest lipid content. This finding indicates that a higher initial concentration might be better for cell growth in upscale cultivation, but not for lipid production, which may be due to the presence of threshold nutrients.
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spelling ums.eprints-320502022-03-29T04:00:17Z https://eprints.ums.edu.my/id/eprint/32050/ Growth and lipid production of isochrysis galbana in an upscale cultivation system Erma Rizah Abdul Sani Thau, Wilson Lym Yong Chin, Grace Joy Wei Lie Mailin Misson QK504-(638) Cryptogams TP315-360 Fuel Microalgae for biofuel production require further research and development to be economically viable, especially in terms of cost and biomass production. These include the need to optimize the favorable growth conditions for low-cost but large-scale cultivation. This study aims to determine the best initial biomass concentration of Isochrysis galbana on a pilot scale cultivation system. Isochrysis galbana was cultured for 69 days in an upscaled 2-liter Erlemenmeyer flask with a variety of initial biomass concentrations using the previously established stock culture (250 ml) at 25°C room temperature, 16:8 light/dark cycle, and 135 µmol/m2/s light intensity. The initial biomass concentration was optimized from a range of 103 cells/ml to 104 cells/ml, 105 cells/ml, and 106 cells/ml. The cell density was calculated every three days to determine the growth curve, and the lipid content was measured weekly throughout the cultivation cycle. The results show that the 106 cells/ml initial concentrations produced the highest growth, but the 104 cells/ml initial concentration produced the highest lipid content. This finding indicates that a higher initial concentration might be better for cell growth in upscale cultivation, but not for lipid production, which may be due to the presence of threshold nutrients. UniSE Press 2021 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/32050/1/Growth%20and%20lipid%20production%20of%20isochrysis%20galbana%20in%20an%20upscale%20cultivation%20system_ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/32050/2/Growth%20and%20lipid%20production%20of%20isochrysis%20galbana%20in%20an%20upscale%20cultivation%20system.pdf Erma Rizah Abdul Sani and Thau, Wilson Lym Yong and Chin, Grace Joy Wei Lie and Mailin Misson (2021) Growth and lipid production of isochrysis galbana in an upscale cultivation system. Transactions on Science and Technology, 8 (2). 203 -209. ISSN 2259-8786 http://tost.unise.org/pdfs/vol8/no3-2/ToST-CoFA2020-203-209-OA.pdf
spellingShingle QK504-(638) Cryptogams
TP315-360 Fuel
Erma Rizah Abdul Sani
Thau, Wilson Lym Yong
Chin, Grace Joy Wei Lie
Mailin Misson
Growth and lipid production of isochrysis galbana in an upscale cultivation system
title Growth and lipid production of isochrysis galbana in an upscale cultivation system
title_full Growth and lipid production of isochrysis galbana in an upscale cultivation system
title_fullStr Growth and lipid production of isochrysis galbana in an upscale cultivation system
title_full_unstemmed Growth and lipid production of isochrysis galbana in an upscale cultivation system
title_short Growth and lipid production of isochrysis galbana in an upscale cultivation system
title_sort growth and lipid production of isochrysis galbana in an upscale cultivation system
topic QK504-(638) Cryptogams
TP315-360 Fuel
url https://eprints.ums.edu.my/id/eprint/32050/1/Growth%20and%20lipid%20production%20of%20isochrysis%20galbana%20in%20an%20upscale%20cultivation%20system_ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/32050/2/Growth%20and%20lipid%20production%20of%20isochrysis%20galbana%20in%20an%20upscale%20cultivation%20system.pdf
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