Growth of harmful dinoflagellate margalefidinium polykrikoides in different nutrient concentrations
Margalefidinium polykrikoides blooms resulted in massive fish mortality in many countries around the world. The prevalence of booms is believed to be associated with the eutrophication of the coastal area. Therefore, this study aims to determine the effects of different nitrogen (N) and phosphorus (...
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| Format: | Article |
| Language: | English English |
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Department of Science and Technology
2022
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| Online Access: | https://eprints.ums.edu.my/id/eprint/32441/1/Growth%20of%20harmful%20dinoflagellate%20margalefidinium%20polykrikoides%20in%20different%20nutrient%20concentrations.pdf https://eprints.ums.edu.my/id/eprint/32441/2/Growth%20of%20harmful%20dinoflagellate%20margalefidinium%20polykrikoides%20in%20different%20nutrient%20concentrations%20_ABSTRACT.pdf |
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| author | Asilah Al-Has Normawaty Mohammad-Noor Sitti Raehanah Muhamad Shaleh Aimimuliani Adam Wan Arina Azrinor Encik Weliyadi |
| author_facet | Asilah Al-Has Normawaty Mohammad-Noor Sitti Raehanah Muhamad Shaleh Aimimuliani Adam Wan Arina Azrinor Encik Weliyadi |
| author_sort | Asilah Al-Has |
| collection | UMS |
| description | Margalefidinium polykrikoides blooms resulted in massive fish mortality in many countries around the world. The prevalence of booms is believed to be associated with the eutrophication of the coastal area. Therefore, this study aims to determine the effects of different nitrogen (N) and phosphorus (P) concentrations on the growth of M. polykrikoides. Experiments were conducted on M. polykrikoides isolated from the affected area by exposing the cell to various N and P ratio: seawater without the addition of N and P (SW), equal N:P ratio (NP), trial ratio of N and P (TR), F/2 medium (+NP), deficient N (–N), and deficient P (–P). The experiments were carried out in triplicates for 20 d in similar environmental conditions with the determination of density, specific growth rate, and cell size during the study period. Furthermore, nutrient concentrations nitrite+nitrate (NO2–+NO3–), phosphate (PO4 3–), and ammonia (NH4 +) were measured initially in the medium and both nutrients and chlorophyll were determined at the end of the experiment. The results revealed that M. polykrikoides grew in all the experiments, but the growth patterns differed between the treatments. The highest specific growth rate (0.1901 ± 0.017 d–1) with the highest cell density (1709 ± 68.21 cells mL–1) with a long chain of cells was observed in TR during the exponential phase. However, cell size was significantly smaller in SW compared to other nutrient conditions during the exponential phase. This study shows that M. polykrikoides a flexible species in nutrient uptake, thus allowing the species to survive in different nutrient conditions. The understanding of this bloom mechanism is important in monitoring and management of this harmful species, particularly in Sabah coastal waters. |
| first_indexed | 2024-03-06T03:15:37Z |
| format | Article |
| id | ums.eprints-32441 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:15:37Z |
| publishDate | 2022 |
| publisher | Department of Science and Technology |
| record_format | dspace |
| spelling | ums.eprints-324412022-04-25T03:25:50Z https://eprints.ums.edu.my/id/eprint/32441/ Growth of harmful dinoflagellate margalefidinium polykrikoides in different nutrient concentrations Asilah Al-Has Normawaty Mohammad-Noor Sitti Raehanah Muhamad Shaleh Aimimuliani Adam Wan Arina Azrinor Encik Weliyadi QE1-996.5 Geology QK1-989 Botany Margalefidinium polykrikoides blooms resulted in massive fish mortality in many countries around the world. The prevalence of booms is believed to be associated with the eutrophication of the coastal area. Therefore, this study aims to determine the effects of different nitrogen (N) and phosphorus (P) concentrations on the growth of M. polykrikoides. Experiments were conducted on M. polykrikoides isolated from the affected area by exposing the cell to various N and P ratio: seawater without the addition of N and P (SW), equal N:P ratio (NP), trial ratio of N and P (TR), F/2 medium (+NP), deficient N (–N), and deficient P (–P). The experiments were carried out in triplicates for 20 d in similar environmental conditions with the determination of density, specific growth rate, and cell size during the study period. Furthermore, nutrient concentrations nitrite+nitrate (NO2–+NO3–), phosphate (PO4 3–), and ammonia (NH4 +) were measured initially in the medium and both nutrients and chlorophyll were determined at the end of the experiment. The results revealed that M. polykrikoides grew in all the experiments, but the growth patterns differed between the treatments. The highest specific growth rate (0.1901 ± 0.017 d–1) with the highest cell density (1709 ± 68.21 cells mL–1) with a long chain of cells was observed in TR during the exponential phase. However, cell size was significantly smaller in SW compared to other nutrient conditions during the exponential phase. This study shows that M. polykrikoides a flexible species in nutrient uptake, thus allowing the species to survive in different nutrient conditions. The understanding of this bloom mechanism is important in monitoring and management of this harmful species, particularly in Sabah coastal waters. Department of Science and Technology 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/32441/1/Growth%20of%20harmful%20dinoflagellate%20margalefidinium%20polykrikoides%20in%20different%20nutrient%20concentrations.pdf text en https://eprints.ums.edu.my/id/eprint/32441/2/Growth%20of%20harmful%20dinoflagellate%20margalefidinium%20polykrikoides%20in%20different%20nutrient%20concentrations%20_ABSTRACT.pdf Asilah Al-Has and Normawaty Mohammad-Noor and Sitti Raehanah Muhamad Shaleh and Aimimuliani Adam and Wan Arina Azrinor and Encik Weliyadi (2022) Growth of harmful dinoflagellate margalefidinium polykrikoides in different nutrient concentrations. Philippine Journal of Science, 151. pp. 945-954. ISSN 0031-7683 https://philjournalsci.dost.gov.ph/images/pdf/pjs_pdf/vol151no3/growth_of_harmful_dinoflagellate_margalefidinium_polykrikoides_.pdf |
| spellingShingle | QE1-996.5 Geology QK1-989 Botany Asilah Al-Has Normawaty Mohammad-Noor Sitti Raehanah Muhamad Shaleh Aimimuliani Adam Wan Arina Azrinor Encik Weliyadi Growth of harmful dinoflagellate margalefidinium polykrikoides in different nutrient concentrations |
| title | Growth of harmful dinoflagellate margalefidinium polykrikoides in different nutrient concentrations |
| title_full | Growth of harmful dinoflagellate margalefidinium polykrikoides in different nutrient concentrations |
| title_fullStr | Growth of harmful dinoflagellate margalefidinium polykrikoides in different nutrient concentrations |
| title_full_unstemmed | Growth of harmful dinoflagellate margalefidinium polykrikoides in different nutrient concentrations |
| title_short | Growth of harmful dinoflagellate margalefidinium polykrikoides in different nutrient concentrations |
| title_sort | growth of harmful dinoflagellate margalefidinium polykrikoides in different nutrient concentrations |
| topic | QE1-996.5 Geology QK1-989 Botany |
| url | https://eprints.ums.edu.my/id/eprint/32441/1/Growth%20of%20harmful%20dinoflagellate%20margalefidinium%20polykrikoides%20in%20different%20nutrient%20concentrations.pdf https://eprints.ums.edu.my/id/eprint/32441/2/Growth%20of%20harmful%20dinoflagellate%20margalefidinium%20polykrikoides%20in%20different%20nutrient%20concentrations%20_ABSTRACT.pdf |
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