In vitro antibacterial activity of marine microalgae extract against vibrio harveyi
Marine microalgae may produce antibacterial substances. At the exponential phase of growth, four species of marine microalgae were examined for their potential to create secondary metabolites that limit the growth of Vibrio harveyi: Nannochloropsis oceanica, Chaetoceros gracilis, Isochrysis sp. and...
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| Format: | Article |
| Language: | English English |
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Multidisciplinary Digital Publishing Institute (MDPI)
2022
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| Online Access: | https://eprints.ums.edu.my/id/eprint/32446/1/In%20vitro%20antibacterial%20activity%20of%20marine%20microalgae%20extract%20against%20vibrio%20harveyi%20_ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/32446/3/In%20vitro%20antibacterial%20activity%20of%20marine%20microalgae%20extract%20against%20vibrio%20harveyi%20_ABSTRACT.pdf |
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| author | Mohd Ridzwan Jusidin Rafidah Othman Sitti Raehanah Muhamad Shaleh Ching Fui Fui @ Faihana Ching Abdullah Shigeharu Senoo Siti Nur Hazwani Oslan |
| author_facet | Mohd Ridzwan Jusidin Rafidah Othman Sitti Raehanah Muhamad Shaleh Ching Fui Fui @ Faihana Ching Abdullah Shigeharu Senoo Siti Nur Hazwani Oslan |
| author_sort | Mohd Ridzwan Jusidin |
| collection | UMS |
| description | Marine microalgae may produce antibacterial substances. At the exponential phase of growth, four species of marine microalgae were examined for their potential to create secondary metabolites that limit the growth of Vibrio harveyi: Nannochloropsis oceanica, Chaetoceros gracilis, Isochrysis sp. and Thalassiosira weissflogii. V. harveyi is a pathogenic bacteria that can cause severe mortality and loss in aquaculture. Disc diffusion assay and co-culture assay were used to determine antibacterial activity. On TSA % NaCl media, the disc impregnated with microalgae and extracted with ethanol, methanol, saline water, and dimethyl sulfoxide (DMSO) was tested against V. harveyi at concentrations of 1.0 × 105 , 106 and 107 CFU mL−1 . The disc diffusion assay revealed that N. oceanica extracted with ethanol had the largest inhibitory zone against V. harveyi. Meanwhile, only N. oceanica, Isochrysis sp. and T. weissflogii reduced the growth of V. harveyi (105 CFU mL−1 ) in the co-culture assay (p < 0.05). The current findings reveal that the hydrophilic chemicals in microalgae extract have antibiotic activity against the highly virulent V. harveyi, which causes vibriosis, a serious disease in farmed fish and aquaculture cultivation around the world. |
| first_indexed | 2024-03-06T03:15:38Z |
| format | Article |
| id | ums.eprints-32446 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:15:38Z |
| publishDate | 2022 |
| publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
| record_format | dspace |
| spelling | ums.eprints-324462022-04-25T03:32:50Z https://eprints.ums.edu.my/id/eprint/32446/ In vitro antibacterial activity of marine microalgae extract against vibrio harveyi Mohd Ridzwan Jusidin Rafidah Othman Sitti Raehanah Muhamad Shaleh Ching Fui Fui @ Faihana Ching Abdullah Shigeharu Senoo Siti Nur Hazwani Oslan QK1-989 Botany Marine microalgae may produce antibacterial substances. At the exponential phase of growth, four species of marine microalgae were examined for their potential to create secondary metabolites that limit the growth of Vibrio harveyi: Nannochloropsis oceanica, Chaetoceros gracilis, Isochrysis sp. and Thalassiosira weissflogii. V. harveyi is a pathogenic bacteria that can cause severe mortality and loss in aquaculture. Disc diffusion assay and co-culture assay were used to determine antibacterial activity. On TSA % NaCl media, the disc impregnated with microalgae and extracted with ethanol, methanol, saline water, and dimethyl sulfoxide (DMSO) was tested against V. harveyi at concentrations of 1.0 × 105 , 106 and 107 CFU mL−1 . The disc diffusion assay revealed that N. oceanica extracted with ethanol had the largest inhibitory zone against V. harveyi. Meanwhile, only N. oceanica, Isochrysis sp. and T. weissflogii reduced the growth of V. harveyi (105 CFU mL−1 ) in the co-culture assay (p < 0.05). The current findings reveal that the hydrophilic chemicals in microalgae extract have antibiotic activity against the highly virulent V. harveyi, which causes vibriosis, a serious disease in farmed fish and aquaculture cultivation around the world. Multidisciplinary Digital Publishing Institute (MDPI) 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/32446/1/In%20vitro%20antibacterial%20activity%20of%20marine%20microalgae%20extract%20against%20vibrio%20harveyi%20_ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/32446/3/In%20vitro%20antibacterial%20activity%20of%20marine%20microalgae%20extract%20against%20vibrio%20harveyi%20_ABSTRACT.pdf Mohd Ridzwan Jusidin and Rafidah Othman and Sitti Raehanah Muhamad Shaleh and Ching Fui Fui @ Faihana Ching Abdullah and Shigeharu Senoo and Siti Nur Hazwani Oslan (2022) In vitro antibacterial activity of marine microalgae extract against vibrio harveyi. Applied Sciences, 12. pp. 1-11. ISSN 2076-3417 https://www.mdpi.com/2076-3417/12/3/1148/htm https://doi.org/10.3390/app12031148 https://doi.org/10.3390/app12031148 |
| spellingShingle | QK1-989 Botany Mohd Ridzwan Jusidin Rafidah Othman Sitti Raehanah Muhamad Shaleh Ching Fui Fui @ Faihana Ching Abdullah Shigeharu Senoo Siti Nur Hazwani Oslan In vitro antibacterial activity of marine microalgae extract against vibrio harveyi |
| title | In vitro antibacterial activity of marine microalgae extract against vibrio harveyi |
| title_full | In vitro antibacterial activity of marine microalgae extract against vibrio harveyi |
| title_fullStr | In vitro antibacterial activity of marine microalgae extract against vibrio harveyi |
| title_full_unstemmed | In vitro antibacterial activity of marine microalgae extract against vibrio harveyi |
| title_short | In vitro antibacterial activity of marine microalgae extract against vibrio harveyi |
| title_sort | in vitro antibacterial activity of marine microalgae extract against vibrio harveyi |
| topic | QK1-989 Botany |
| url | https://eprints.ums.edu.my/id/eprint/32446/1/In%20vitro%20antibacterial%20activity%20of%20marine%20microalgae%20extract%20against%20vibrio%20harveyi%20_ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/32446/3/In%20vitro%20antibacterial%20activity%20of%20marine%20microalgae%20extract%20against%20vibrio%20harveyi%20_ABSTRACT.pdf |
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