Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis
Marine leech Zeylanicobdella arugamensis (Piscicolidae), an economically important parasite is infesting predominantly cultured groupers, hybrid groupers and other fsh in Southeast Asian countries. In this study, we tested the anti-parasitic potential of a medicinal plant Nephrolepis biserrata found...
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| Format: | Article |
| Language: | English English |
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Nature Publishing
2020
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| Online Access: | https://eprints.ums.edu.my/id/eprint/32457/1/Antiparasitic%20potential%20of%20Nephrolepis%20biserrata%20methanol%20extract%20against%20the%20parasitic%20leech%20Zeylanicobdella%20arugamensis%20%28Hirudinea%29%20and%20LC-QTOF%20analysis.pdf https://eprints.ums.edu.my/id/eprint/32457/2/Antiparasitic%20potential%20of%20Nephrolepis%20biserrata%20methanol%20extract%20against%20the%20parasitic%20leech%20Zeylanicobdella%20arugamensis%20_ABSTRACT.pdf |
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| author | Muhammad Dawood Shah Balu Alagar Venmathi Maran Fatin Khairah Haron Julian Ransangan Ching Fui Fui @ Faihana Ching Abdullah Sitti Raehanah Muhamad Shaleh Rossita Shapawi Yoong Soon Yong Susumu Ohtsuka |
| author_facet | Muhammad Dawood Shah Balu Alagar Venmathi Maran Fatin Khairah Haron Julian Ransangan Ching Fui Fui @ Faihana Ching Abdullah Sitti Raehanah Muhamad Shaleh Rossita Shapawi Yoong Soon Yong Susumu Ohtsuka |
| author_sort | Muhammad Dawood Shah |
| collection | UMS |
| description | Marine leech Zeylanicobdella arugamensis (Piscicolidae), an economically important parasite is infesting predominantly cultured groupers, hybrid groupers and other fsh in Southeast Asian countries. In this study, we tested the anti-parasitic potential of a medicinal plant Nephrolepis biserrata found in Sabah, East Malaysia against Z. arugamensis. Various concentrations of methanol extracts of the plant were tested experimentally against Z. arugamensis and disinfestation of the leech from its primary host hybrid groupers. The composition of methanol extract of N. biserrata was determined through LC-QTOF analysis. The signifcant anti-parasitic activity of 100% mortality of leeches was observed with the exposure of N. biserrata extracts. The average time to kill the leeches at concentrations of 25, 50 and 100 mg/ml was 25.11 ± 3.26, 11.91 ± 0.99, and 4.88 ± 0.50 min., respectively. Further, at various low concentrations of N. biserrata 2.5, 5 and 10 mg/ml, hybrid groupers were disinfested in an average time of 108.33± 12.65, 65.83 ± 9.70 and 29.16 ± 5.85 min., respectively. The tandem mass spectrometry data from LC-QTOF indicated some hits on useful bioactive compounds such as terpenoids (ivalin, isovelleral, brassinolide, and eschscholtzxanthin), favonoids (alnustin, kaempferol 7,4′-dimethyl ether, and pachypodol), phenolics (piscidic acid, chlorogenic acid, and ankorine), and aromatic (3-hydroxycoumarin). Thus N. biserrata can act as a potential biocontrol agent. |
| first_indexed | 2024-03-06T03:15:40Z |
| format | Article |
| id | ums.eprints-32457 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:15:40Z |
| publishDate | 2020 |
| publisher | Nature Publishing |
| record_format | dspace |
| spelling | ums.eprints-324572022-04-25T03:38:22Z https://eprints.ums.edu.my/id/eprint/32457/ Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis Muhammad Dawood Shah Balu Alagar Venmathi Maran Fatin Khairah Haron Julian Ransangan Ching Fui Fui @ Faihana Ching Abdullah Sitti Raehanah Muhamad Shaleh Rossita Shapawi Yoong Soon Yong Susumu Ohtsuka SH1-691 Aquaculture. Fisheries. Angling Marine leech Zeylanicobdella arugamensis (Piscicolidae), an economically important parasite is infesting predominantly cultured groupers, hybrid groupers and other fsh in Southeast Asian countries. In this study, we tested the anti-parasitic potential of a medicinal plant Nephrolepis biserrata found in Sabah, East Malaysia against Z. arugamensis. Various concentrations of methanol extracts of the plant were tested experimentally against Z. arugamensis and disinfestation of the leech from its primary host hybrid groupers. The composition of methanol extract of N. biserrata was determined through LC-QTOF analysis. The signifcant anti-parasitic activity of 100% mortality of leeches was observed with the exposure of N. biserrata extracts. The average time to kill the leeches at concentrations of 25, 50 and 100 mg/ml was 25.11 ± 3.26, 11.91 ± 0.99, and 4.88 ± 0.50 min., respectively. Further, at various low concentrations of N. biserrata 2.5, 5 and 10 mg/ml, hybrid groupers were disinfested in an average time of 108.33± 12.65, 65.83 ± 9.70 and 29.16 ± 5.85 min., respectively. The tandem mass spectrometry data from LC-QTOF indicated some hits on useful bioactive compounds such as terpenoids (ivalin, isovelleral, brassinolide, and eschscholtzxanthin), favonoids (alnustin, kaempferol 7,4′-dimethyl ether, and pachypodol), phenolics (piscidic acid, chlorogenic acid, and ankorine), and aromatic (3-hydroxycoumarin). Thus N. biserrata can act as a potential biocontrol agent. Nature Publishing 2020 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/32457/1/Antiparasitic%20potential%20of%20Nephrolepis%20biserrata%20methanol%20extract%20against%20the%20parasitic%20leech%20Zeylanicobdella%20arugamensis%20%28Hirudinea%29%20and%20LC-QTOF%20analysis.pdf text en https://eprints.ums.edu.my/id/eprint/32457/2/Antiparasitic%20potential%20of%20Nephrolepis%20biserrata%20methanol%20extract%20against%20the%20parasitic%20leech%20Zeylanicobdella%20arugamensis%20_ABSTRACT.pdf Muhammad Dawood Shah and Balu Alagar Venmathi Maran and Fatin Khairah Haron and Julian Ransangan and Ching Fui Fui @ Faihana Ching Abdullah and Sitti Raehanah Muhamad Shaleh and Rossita Shapawi and Yoong Soon Yong and Susumu Ohtsuka (2020) Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis. Scientific Reports, 10. ISSN 2045-2322 https://www.nature.com/articles/s41598-020-79094-4.pdf https://doi.org/10.1038/s41598-020-79094-4 https://doi.org/10.1038/s41598-020-79094-4 |
| spellingShingle | SH1-691 Aquaculture. Fisheries. Angling Muhammad Dawood Shah Balu Alagar Venmathi Maran Fatin Khairah Haron Julian Ransangan Ching Fui Fui @ Faihana Ching Abdullah Sitti Raehanah Muhamad Shaleh Rossita Shapawi Yoong Soon Yong Susumu Ohtsuka Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis |
| title | Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis |
| title_full | Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis |
| title_fullStr | Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis |
| title_full_unstemmed | Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis |
| title_short | Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis |
| title_sort | antiparasitic potential of nephrolepis biserrata methanol extract against the parasitic leech zeylanicobdella arugamensis hirudinea and lc qtof analysis |
| topic | SH1-691 Aquaculture. Fisheries. Angling |
| url | https://eprints.ums.edu.my/id/eprint/32457/1/Antiparasitic%20potential%20of%20Nephrolepis%20biserrata%20methanol%20extract%20against%20the%20parasitic%20leech%20Zeylanicobdella%20arugamensis%20%28Hirudinea%29%20and%20LC-QTOF%20analysis.pdf https://eprints.ums.edu.my/id/eprint/32457/2/Antiparasitic%20potential%20of%20Nephrolepis%20biserrata%20methanol%20extract%20against%20the%20parasitic%20leech%20Zeylanicobdella%20arugamensis%20_ABSTRACT.pdf |
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