Multi-assay investigation of viral etiology in pediatric central nervous system infections
Introduction: In an attempt to identify a wide spectrum of viral infections, cerebrospinal fluid (CSF) specimens were collected from pediatric cases with the preliminary diagnosis of viral encephalitis/meningoencephalitis in two reference hospitals, from October 2011 to December 2015. Methodology: A...
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| Format: | Article |
| Language: | English English |
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Journal of Infection in Developing Countries
2020
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| Online Access: | https://eprints.ums.edu.my/id/eprint/32549/1/Multi-assay%20investigation%20of%20viral%20etiology%20in%20pediatric%20central%20nervous%20system%20infections.pdf https://eprints.ums.edu.my/id/eprint/32549/2/Multi-assay%20investigation%20of%20viral%20etiology%20in%20pediatric%20central%20nervous%20system%20infections%20_ABSTRACT.pdf |
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| author | Aylin Altay-Kocak Gulendam Bozdayi Janine Michel Meltem Polat Saliha Kanik-Yuksek Hasan Tezer Aykut Ozkul Kamruddin Ahmed Andreas Nitsche Koray Ergunay |
| author_facet | Aylin Altay-Kocak Gulendam Bozdayi Janine Michel Meltem Polat Saliha Kanik-Yuksek Hasan Tezer Aykut Ozkul Kamruddin Ahmed Andreas Nitsche Koray Ergunay |
| author_sort | Aylin Altay-Kocak |
| collection | UMS |
| description | Introduction: In an attempt to identify a wide spectrum of viral infections, cerebrospinal fluid (CSF) specimens were collected from pediatric cases with the preliminary diagnosis of viral encephalitis/meningoencephalitis in two reference hospitals, from October 2011 to December 2015. Methodology: A combination of nucleic acid-based assays, including in house generic polymerase chain reaction (PCR) assays for enteroviruses, flaviviruses and phleboviruses, a commercial real-time PCR assay for herpesviruses and a commercial real time multiplex PCR, enabling detection of frequently-observed viral, bacterial and fungal agents were employed for screening. Results: The microbial agent could be characterized in 10 (10%) of the 100 specimens. Viral etiology could be demonstrated in 7 (70%) specimens, which comprises Human Herpesvirus 6 (4/7), Herpes Simplex virus type1 (2/7) and Enteroviruses (1/7). In 3 specimens (30%), Streptococcus pneumoniae, Listeria monocytogenes and Staphylococcus aureus were detected via the multiplex PCR, which were also isolated in bacteriological media. All specimens with detectable viral nucleic acids, as well as unreactive specimens via nucleic acid testing remained negative in bacteriological cultures. Conclusions: Herpes and enteroviruses were identified as the primary causative agents of central nervous system infections in children. Enterovirus testing must be included in the diagnostic work-up of relevant cases. |
| first_indexed | 2024-03-06T03:15:54Z |
| format | Article |
| id | ums.eprints-32549 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:15:54Z |
| publishDate | 2020 |
| publisher | Journal of Infection in Developing Countries |
| record_format | dspace |
| spelling | ums.eprints-325492022-05-04T03:44:59Z https://eprints.ums.edu.my/id/eprint/32549/ Multi-assay investigation of viral etiology in pediatric central nervous system infections Aylin Altay-Kocak Gulendam Bozdayi Janine Michel Meltem Polat Saliha Kanik-Yuksek Hasan Tezer Aykut Ozkul Kamruddin Ahmed Andreas Nitsche Koray Ergunay RJ1-570 Pediatrics Introduction: In an attempt to identify a wide spectrum of viral infections, cerebrospinal fluid (CSF) specimens were collected from pediatric cases with the preliminary diagnosis of viral encephalitis/meningoencephalitis in two reference hospitals, from October 2011 to December 2015. Methodology: A combination of nucleic acid-based assays, including in house generic polymerase chain reaction (PCR) assays for enteroviruses, flaviviruses and phleboviruses, a commercial real-time PCR assay for herpesviruses and a commercial real time multiplex PCR, enabling detection of frequently-observed viral, bacterial and fungal agents were employed for screening. Results: The microbial agent could be characterized in 10 (10%) of the 100 specimens. Viral etiology could be demonstrated in 7 (70%) specimens, which comprises Human Herpesvirus 6 (4/7), Herpes Simplex virus type1 (2/7) and Enteroviruses (1/7). In 3 specimens (30%), Streptococcus pneumoniae, Listeria monocytogenes and Staphylococcus aureus were detected via the multiplex PCR, which were also isolated in bacteriological media. All specimens with detectable viral nucleic acids, as well as unreactive specimens via nucleic acid testing remained negative in bacteriological cultures. Conclusions: Herpes and enteroviruses were identified as the primary causative agents of central nervous system infections in children. Enterovirus testing must be included in the diagnostic work-up of relevant cases. Journal of Infection in Developing Countries 2020 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/32549/1/Multi-assay%20investigation%20of%20viral%20etiology%20in%20pediatric%20central%20nervous%20system%20infections.pdf text en https://eprints.ums.edu.my/id/eprint/32549/2/Multi-assay%20investigation%20of%20viral%20etiology%20in%20pediatric%20central%20nervous%20system%20infections%20_ABSTRACT.pdf Aylin Altay-Kocak and Gulendam Bozdayi and Janine Michel and Meltem Polat and Saliha Kanik-Yuksek and Hasan Tezer and Aykut Ozkul and Kamruddin Ahmed and Andreas Nitsche and Koray Ergunay (2020) Multi-assay investigation of viral etiology in pediatric central nervous system infections. Journal of infection in developing countries, 14. pp. 572-579. ISSN 2036-6590 (P-ISSN) , 1972-2680 (E-ISSN) https://jidc.org/index.php/journal/article/view/12327 https://doi.org/10.3855/jidc.12327 https://doi.org/10.3855/jidc.12327 |
| spellingShingle | RJ1-570 Pediatrics Aylin Altay-Kocak Gulendam Bozdayi Janine Michel Meltem Polat Saliha Kanik-Yuksek Hasan Tezer Aykut Ozkul Kamruddin Ahmed Andreas Nitsche Koray Ergunay Multi-assay investigation of viral etiology in pediatric central nervous system infections |
| title | Multi-assay investigation of viral etiology in pediatric central nervous system infections |
| title_full | Multi-assay investigation of viral etiology in pediatric central nervous system infections |
| title_fullStr | Multi-assay investigation of viral etiology in pediatric central nervous system infections |
| title_full_unstemmed | Multi-assay investigation of viral etiology in pediatric central nervous system infections |
| title_short | Multi-assay investigation of viral etiology in pediatric central nervous system infections |
| title_sort | multi assay investigation of viral etiology in pediatric central nervous system infections |
| topic | RJ1-570 Pediatrics |
| url | https://eprints.ums.edu.my/id/eprint/32549/1/Multi-assay%20investigation%20of%20viral%20etiology%20in%20pediatric%20central%20nervous%20system%20infections.pdf https://eprints.ums.edu.my/id/eprint/32549/2/Multi-assay%20investigation%20of%20viral%20etiology%20in%20pediatric%20central%20nervous%20system%20infections%20_ABSTRACT.pdf |
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