Genome-wide association study of myrtle rust (Austropuccinia psidii) resistance in Eucalyptus obliqua (subgenus Eucalyptus)
Myrtle rust (caused by Austropuccinia psidii Beenken) is exotic to Australia, yet specific resistance is present in a range of naïve myrtaceous host plants. Resistance to myrtle rust is primarily quantitative in nature and controlled by multiple interacting loci. We undertook a genome-wide associati...
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| Format: | Article |
| Language: | English English |
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Springer
2021
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| Online Access: | https://eprints.ums.edu.my/id/eprint/32904/1/Genome-wide%20association%20study%20of%20myrtle%20rust%20%28Austropuccinia%20psidii%29%20resistance%20in%20Eucalyptus%20obliqua%20%28subgenus%20Eucalyptus%29.pdf https://eprints.ums.edu.my/id/eprint/32904/2/Genome-wide%20association%20study%20of%20myrtle%20rust%20%28Austropuccinia%20psidii%29%20resistance%20in%20Eucalyptus%20obliqua%20%28subgenus%20Eucalyptus%291.pdf |
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| author | Thau, Wilson Lym Yong Peter K. Ades Fatima Akter Runa Gerd Bossinger Karanjeet S. Sandhu Brad M. Potts Josquin F.G. Tibbits |
| author_facet | Thau, Wilson Lym Yong Peter K. Ades Fatima Akter Runa Gerd Bossinger Karanjeet S. Sandhu Brad M. Potts Josquin F.G. Tibbits |
| author_sort | Thau, Wilson Lym Yong |
| collection | UMS |
| description | Myrtle rust (caused by Austropuccinia psidii Beenken) is exotic to Australia, yet specific resistance is present in a range of naïve myrtaceous host plants. Resistance to myrtle rust is primarily quantitative in nature and controlled by multiple interacting loci. We undertook a genome-wide association study (GWAS) to discover relationships between nuclear genomic sequence variation and multiple aspects of resistance to A. psidii in Eucalyptus obliqua, a representative species from subgenus Eucalyptus. Sequence variation was assessed with respect to numeric severity, binary symptomatic, hypersensitive, and pustulation responses to inoculation of seedlings with A. psidii. A total of 1.13 million, single-nucleotide polymorphisms (SNPs) were tested for association with the rust resistance responses of 637 phenotyped E. obliqua seedlings, each from different, single mother tree seedlots. Thirty-three highly significant SNP-trait associations were detected, of which 26 associated with the binary symptomatic/asymptomatic response to A. psidii. Comparison of the genomic position of these SNPs with rust resistance loci (Ppr1-5), previously reported in species from subgenus Symphyomyrtus, suggested that several were positioned near the major Ppr1 locus and other Ppr loci. This study provides the first integrated genomic view of A. psidii resistance across the Eucalyptus subgenera and provides the foundation for discovering key resistance genes for use in marker-based resistance breeding. |
| first_indexed | 2024-03-06T03:16:53Z |
| format | Article |
| id | ums.eprints-32904 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:16:53Z |
| publishDate | 2021 |
| publisher | Springer |
| record_format | dspace |
| spelling | ums.eprints-329042022-06-21T01:33:03Z https://eprints.ums.edu.my/id/eprint/32904/ Genome-wide association study of myrtle rust (Austropuccinia psidii) resistance in Eucalyptus obliqua (subgenus Eucalyptus) Thau, Wilson Lym Yong Peter K. Ades Fatima Akter Runa Gerd Bossinger Karanjeet S. Sandhu Brad M. Potts Josquin F.G. Tibbits QK1-989 Botany QK640-(707) Plant anatomy Myrtle rust (caused by Austropuccinia psidii Beenken) is exotic to Australia, yet specific resistance is present in a range of naïve myrtaceous host plants. Resistance to myrtle rust is primarily quantitative in nature and controlled by multiple interacting loci. We undertook a genome-wide association study (GWAS) to discover relationships between nuclear genomic sequence variation and multiple aspects of resistance to A. psidii in Eucalyptus obliqua, a representative species from subgenus Eucalyptus. Sequence variation was assessed with respect to numeric severity, binary symptomatic, hypersensitive, and pustulation responses to inoculation of seedlings with A. psidii. A total of 1.13 million, single-nucleotide polymorphisms (SNPs) were tested for association with the rust resistance responses of 637 phenotyped E. obliqua seedlings, each from different, single mother tree seedlots. Thirty-three highly significant SNP-trait associations were detected, of which 26 associated with the binary symptomatic/asymptomatic response to A. psidii. Comparison of the genomic position of these SNPs with rust resistance loci (Ppr1-5), previously reported in species from subgenus Symphyomyrtus, suggested that several were positioned near the major Ppr1 locus and other Ppr loci. This study provides the first integrated genomic view of A. psidii resistance across the Eucalyptus subgenera and provides the foundation for discovering key resistance genes for use in marker-based resistance breeding. Springer 2021 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/32904/1/Genome-wide%20association%20study%20of%20myrtle%20rust%20%28Austropuccinia%20psidii%29%20resistance%20in%20Eucalyptus%20obliqua%20%28subgenus%20Eucalyptus%29.pdf text en https://eprints.ums.edu.my/id/eprint/32904/2/Genome-wide%20association%20study%20of%20myrtle%20rust%20%28Austropuccinia%20psidii%29%20resistance%20in%20Eucalyptus%20obliqua%20%28subgenus%20Eucalyptus%291.pdf Thau, Wilson Lym Yong and Peter K. Ades and Fatima Akter Runa and Gerd Bossinger and Karanjeet S. Sandhu and Brad M. Potts and Josquin F.G. Tibbits (2021) Genome-wide association study of myrtle rust (Austropuccinia psidii) resistance in Eucalyptus obliqua (subgenus Eucalyptus). Tree Genetics and Genomes, 17 (31). pp. 1-18. ISSN 1614-2950 https://link.springer.com/article/10.1007/s11295-021-01511-0 https://doi.org/10.1007/s11295-021-01511-0 https://doi.org/10.1007/s11295-021-01511-0 |
| spellingShingle | QK1-989 Botany QK640-(707) Plant anatomy Thau, Wilson Lym Yong Peter K. Ades Fatima Akter Runa Gerd Bossinger Karanjeet S. Sandhu Brad M. Potts Josquin F.G. Tibbits Genome-wide association study of myrtle rust (Austropuccinia psidii) resistance in Eucalyptus obliqua (subgenus Eucalyptus) |
| title | Genome-wide association study of myrtle rust (Austropuccinia psidii) resistance in Eucalyptus obliqua (subgenus Eucalyptus) |
| title_full | Genome-wide association study of myrtle rust (Austropuccinia psidii) resistance in Eucalyptus obliqua (subgenus Eucalyptus) |
| title_fullStr | Genome-wide association study of myrtle rust (Austropuccinia psidii) resistance in Eucalyptus obliqua (subgenus Eucalyptus) |
| title_full_unstemmed | Genome-wide association study of myrtle rust (Austropuccinia psidii) resistance in Eucalyptus obliqua (subgenus Eucalyptus) |
| title_short | Genome-wide association study of myrtle rust (Austropuccinia psidii) resistance in Eucalyptus obliqua (subgenus Eucalyptus) |
| title_sort | genome wide association study of myrtle rust austropuccinia psidii resistance in eucalyptus obliqua subgenus eucalyptus |
| topic | QK1-989 Botany QK640-(707) Plant anatomy |
| url | https://eprints.ums.edu.my/id/eprint/32904/1/Genome-wide%20association%20study%20of%20myrtle%20rust%20%28Austropuccinia%20psidii%29%20resistance%20in%20Eucalyptus%20obliqua%20%28subgenus%20Eucalyptus%29.pdf https://eprints.ums.edu.my/id/eprint/32904/2/Genome-wide%20association%20study%20of%20myrtle%20rust%20%28Austropuccinia%20psidii%29%20resistance%20in%20Eucalyptus%20obliqua%20%28subgenus%20Eucalyptus%291.pdf |
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