Genomic Molecular Markers for the DNA Fingerprinting of Sperm Derived from Epinephelus lanceolatus and E. fuscoguttatus
Groupers belong to the subfamily Epinephelinae of the family Serranidae. They are an economically important marine fisheries resource and are commercially cultivated throughout the tropical and temperate regions of the world. The aquaculture industry relies on artificial breeding of groupers in orde...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
SCIENCEDOMAIN international
2014
|
| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/33236/1/Genomic%20Molecular%20Markers%20for%20the%20DNA%20Fingerprinting%20of%20Sperm%20Derived%20from%20Epinephelus%20lanceolatus%20and%20E.%20fuscoguttatus.pdf https://eprints.ums.edu.my/id/eprint/33236/2/Genomic%20Molecular%20Markers%20for%20the%20DNA%20Fingerprinting%20of%20Sperm%20Derived%20from%20Epinephelus%20lanceolatus%20and%20E.%20fuscoguttatus1.pdf |
| _version_ | 1825714844792258560 |
|---|---|
| author | Kenneth Francis Rodrigues Shafiquzzaman Siddiquee Shigeharu Senoo |
| author_facet | Kenneth Francis Rodrigues Shafiquzzaman Siddiquee Shigeharu Senoo |
| author_sort | Kenneth Francis Rodrigues |
| collection | UMS |
| description | Groupers belong to the subfamily Epinephelinae of the family Serranidae. They are an economically important marine fisheries resource and are commercially cultivated throughout the tropical and temperate regions of the world. The aquaculture industry relies on artificial breeding of groupers in order to obtain fingerlings which are free of pathogens and demonstrate a uniform growth rate. Rapid validation of sperm and eggs is a major challenge to breeders. Single locus DNA markers are ideal for the authentication of germplasm as they generate single PCR amplicons which do not require further sequencing. This study focused on the development of single locus DNA markers for genotyping of sperm samples derived from two species of grouper, the Giant grouper (Epinephelus lanceolatus) and the Tiger grouper (E. fuscoguttatus). Single locus molecular markers were developed using DNA sequences obtained from shotgun genomic libraries and tested against sperm samples derived from each of the species and the closely related groupers E. coioides and E. corallicola. A total of 54 molecular markers were developed ofwhich six were found to be specific to E. fuscoguttatus and seven to E. lanceolatus. The remaining markers generated PCR products in all of the four species and were rejected as suitable candidates for genotyping. The markers developed as a result of this study are relevant to fish breeders and fish farmers as they species specific, inexpensive and augment traditional methods of identification based on phenotypic characterization. |
| first_indexed | 2024-03-06T03:17:48Z |
| format | Article |
| id | ums.eprints-33236 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:17:48Z |
| publishDate | 2014 |
| publisher | SCIENCEDOMAIN international |
| record_format | dspace |
| spelling | ums.eprints-332362022-07-16T02:56:47Z https://eprints.ums.edu.my/id/eprint/33236/ Genomic Molecular Markers for the DNA Fingerprinting of Sperm Derived from Epinephelus lanceolatus and E. fuscoguttatus Kenneth Francis Rodrigues Shafiquzzaman Siddiquee Shigeharu Senoo QL614-639.8 Fishes Groupers belong to the subfamily Epinephelinae of the family Serranidae. They are an economically important marine fisheries resource and are commercially cultivated throughout the tropical and temperate regions of the world. The aquaculture industry relies on artificial breeding of groupers in order to obtain fingerlings which are free of pathogens and demonstrate a uniform growth rate. Rapid validation of sperm and eggs is a major challenge to breeders. Single locus DNA markers are ideal for the authentication of germplasm as they generate single PCR amplicons which do not require further sequencing. This study focused on the development of single locus DNA markers for genotyping of sperm samples derived from two species of grouper, the Giant grouper (Epinephelus lanceolatus) and the Tiger grouper (E. fuscoguttatus). Single locus molecular markers were developed using DNA sequences obtained from shotgun genomic libraries and tested against sperm samples derived from each of the species and the closely related groupers E. coioides and E. corallicola. A total of 54 molecular markers were developed ofwhich six were found to be specific to E. fuscoguttatus and seven to E. lanceolatus. The remaining markers generated PCR products in all of the four species and were rejected as suitable candidates for genotyping. The markers developed as a result of this study are relevant to fish breeders and fish farmers as they species specific, inexpensive and augment traditional methods of identification based on phenotypic characterization. SCIENCEDOMAIN international 2014 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/33236/1/Genomic%20Molecular%20Markers%20for%20the%20DNA%20Fingerprinting%20of%20Sperm%20Derived%20from%20Epinephelus%20lanceolatus%20and%20E.%20fuscoguttatus.pdf text en https://eprints.ums.edu.my/id/eprint/33236/2/Genomic%20Molecular%20Markers%20for%20the%20DNA%20Fingerprinting%20of%20Sperm%20Derived%20from%20Epinephelus%20lanceolatus%20and%20E.%20fuscoguttatus1.pdf Kenneth Francis Rodrigues and Shafiquzzaman Siddiquee and Shigeharu Senoo (2014) Genomic Molecular Markers for the DNA Fingerprinting of Sperm Derived from Epinephelus lanceolatus and E. fuscoguttatus. Annual Research & Review in Biology, 4. pp. 93-104. ISSN 2347-565X https://journalarrb.com/index.php/ARRB/article/view/26521/49763 |
| spellingShingle | QL614-639.8 Fishes Kenneth Francis Rodrigues Shafiquzzaman Siddiquee Shigeharu Senoo Genomic Molecular Markers for the DNA Fingerprinting of Sperm Derived from Epinephelus lanceolatus and E. fuscoguttatus |
| title | Genomic Molecular Markers for the DNA Fingerprinting of Sperm Derived from Epinephelus lanceolatus and E. fuscoguttatus |
| title_full | Genomic Molecular Markers for the DNA Fingerprinting of Sperm Derived from Epinephelus lanceolatus and E. fuscoguttatus |
| title_fullStr | Genomic Molecular Markers for the DNA Fingerprinting of Sperm Derived from Epinephelus lanceolatus and E. fuscoguttatus |
| title_full_unstemmed | Genomic Molecular Markers for the DNA Fingerprinting of Sperm Derived from Epinephelus lanceolatus and E. fuscoguttatus |
| title_short | Genomic Molecular Markers for the DNA Fingerprinting of Sperm Derived from Epinephelus lanceolatus and E. fuscoguttatus |
| title_sort | genomic molecular markers for the dna fingerprinting of sperm derived from epinephelus lanceolatus and e fuscoguttatus |
| topic | QL614-639.8 Fishes |
| url | https://eprints.ums.edu.my/id/eprint/33236/1/Genomic%20Molecular%20Markers%20for%20the%20DNA%20Fingerprinting%20of%20Sperm%20Derived%20from%20Epinephelus%20lanceolatus%20and%20E.%20fuscoguttatus.pdf https://eprints.ums.edu.my/id/eprint/33236/2/Genomic%20Molecular%20Markers%20for%20the%20DNA%20Fingerprinting%20of%20Sperm%20Derived%20from%20Epinephelus%20lanceolatus%20and%20E.%20fuscoguttatus1.pdf |
| work_keys_str_mv | AT kennethfrancisrodrigues genomicmolecularmarkersforthednafingerprintingofspermderivedfromepinepheluslanceolatusandefuscoguttatus AT shafiquzzamansiddiquee genomicmolecularmarkersforthednafingerprintingofspermderivedfromepinepheluslanceolatusandefuscoguttatus AT shigeharusenoo genomicmolecularmarkersforthednafingerprintingofspermderivedfromepinepheluslanceolatusandefuscoguttatus |