Morphological Changes of G. boninense Mycelia after being Challenges with Thiram, ZNO NPS and Lucsin

Basal Stem Rot (BSR)disease is one of the major threats to the oil palm industry in Southeast Asia. This paper aims to find control to BSR using thiram, Zinc Oxide nanoparticles (ZnO NPs) and Lucsin. The study was carried out in vitro to assess the antifungal effect of these agents by percentage inh...

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Bibliographic Details
Main Authors: Elleiancly Yuli, Jedol Dayou, Chong, Khim Phin
Format: Article
Language:English
English
Published: Microscopy Society Malaysia 2022
Subjects:
Online Access:https://eprints.ums.edu.my/id/eprint/33528/1/Morphological%20Changes%20of%20G.%20boninense%20Mycelia%20after%20being%20Challenges%20with%20Thiram%2C%20ZNO%20NPS%20and%20Lucsin.pdf
https://eprints.ums.edu.my/id/eprint/33528/2/Morphological%20Changes%20of%20G.%20boninense%20Mycelia%20after%20being%20Challenges%20with%20Thiram%2C%20ZNO%20NPS%20and%20Lucsin1.pdf
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Summary:Basal Stem Rot (BSR)disease is one of the major threats to the oil palm industry in Southeast Asia. This paper aims to find control to BSR using thiram, Zinc Oxide nanoparticles (ZnO NPs) and Lucsin. The study was carried out in vitro to assess the antifungal effect of these agents by percentage inhibition radius growth with the observation of their morphology under Scanning Electron Microscope (SEM)after treatment. From our observation, thiram could fully in hibit G. boninense radial growth, while ZnO NPsup to 80%. No inhibition was recorded with Lucsin. Observation under SEM confirmed G. boninense mycelia after treated with thiram shown abnormality, distorted and damaged hyphal which had resulted in less branches of the fungal mat. There are also evidences of less branches of fungal mat as well as malformation structures of G.boninense hyphae after being treated by ZnO NPs. Only minor ruptures was found on the hyphae, while the hyphal branch were shriveled, distorted and flattened when challenged with Lucsin. In summary, thiram shows potential for complete inhibition of G.boninense while ZnO NPs has slightly lower inhibition potential but Lucsin is incapable of providing significant control to the growth of G.boninense.