High‐throughput metabolomics reveals dysregulation of hydrophobic metabolomes in cancer cell lines by Eleusine indica
Eleusine indica, which is used in traditional medicine, exhibits antiproliferative activity against several cancer cell lines. However, metabolomic studies to evaluate the metabolite changes induced by E. indica in cancer cells are still lacking. The present study investigated the anticancer effects...
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| Format: | Article |
| Language: | English English |
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Nature Publishing Group
2022
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| Online Access: | https://eprints.ums.edu.my/id/eprint/33758/2/High%E2%80%90throughput%20metabolomics%20reveals%20dysregulation%20of%20hydrophobic%20metabolomes%20in%20cancer%20cell%20lines%20by%20Eleusine%20indica.pdf https://eprints.ums.edu.my/id/eprint/33758/1/High%E2%80%90throughput%20metabolomics%20reveals%20dysregulation%20of%20hydrophobic%20metabolomes%20in%20cancer%20cell%20lines%20by%20Eleusine%20indica%20_ABSTRACT.pdf |
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| author | Perng Yang Puah Jiunn, Dexter Herng Lee Soo, Huan Puah Nik Amin Sahid Nik Lah Yee, Soon Ling Fong, Alison Siat Yee |
| author_facet | Perng Yang Puah Jiunn, Dexter Herng Lee Soo, Huan Puah Nik Amin Sahid Nik Lah Yee, Soon Ling Fong, Alison Siat Yee |
| author_sort | Perng Yang Puah |
| collection | UMS |
| description | Eleusine indica, which is used in traditional medicine, exhibits antiproliferative activity against several cancer cell lines. However, metabolomic studies to evaluate the metabolite changes induced by E. indica in cancer cells are still lacking. The present study investigated the anticancer effects of a root fraction of E. indica (R-S5-C1-H1) on H1299, MCF-7, and SK-HEP-1 cell lines and analyzed metabolic changes in the treated cancer cells using ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS). Cell metabolic activity assays demonstrated that the cell viability of the three cancer cell lines was significantly reduced following treatment with R-S5-C1-H1, with half-maximal inhibitory concentrations values of 12.95 µg/mL, 15.99 µg/mL, and 13.69 µg/mL at 72 h, respectively. Microscopy analysis using Hoechst 33342 and Annexin V fluorescent dyes revealed that cells treated with R-S5-C1-H1 underwent apoptotic cell death, while chemometric analysis suggested that apoptosis was triggered 48 h after treatment with R-S5-C1-H1. Deconvoluted cellular metabolomics revealed that hydrophobic metabolites were significantly altered, including triacylglycerols, phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, and ceramide, suggesting that apoptosis induction by R-S5-C1-H1 potentially occurred through modulation of phospholipid synthesis and sphingolipid metabolism. These metabolomic profiling results provide new insights into the anticancer mechanisms of E. indica and facilitate the overall understanding of molecular events following therapeutic interventions. |
| first_indexed | 2024-03-06T03:19:09Z |
| format | Article |
| id | ums.eprints-33758 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:19:09Z |
| publishDate | 2022 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | ums.eprints-337582022-08-15T03:38:38Z https://eprints.ums.edu.my/id/eprint/33758/ High‐throughput metabolomics reveals dysregulation of hydrophobic metabolomes in cancer cell lines by Eleusine indica Perng Yang Puah Jiunn, Dexter Herng Lee Soo, Huan Puah Nik Amin Sahid Nik Lah Yee, Soon Ling Fong, Alison Siat Yee RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens RS153-441 Materia medica Eleusine indica, which is used in traditional medicine, exhibits antiproliferative activity against several cancer cell lines. However, metabolomic studies to evaluate the metabolite changes induced by E. indica in cancer cells are still lacking. The present study investigated the anticancer effects of a root fraction of E. indica (R-S5-C1-H1) on H1299, MCF-7, and SK-HEP-1 cell lines and analyzed metabolic changes in the treated cancer cells using ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS). Cell metabolic activity assays demonstrated that the cell viability of the three cancer cell lines was significantly reduced following treatment with R-S5-C1-H1, with half-maximal inhibitory concentrations values of 12.95 µg/mL, 15.99 µg/mL, and 13.69 µg/mL at 72 h, respectively. Microscopy analysis using Hoechst 33342 and Annexin V fluorescent dyes revealed that cells treated with R-S5-C1-H1 underwent apoptotic cell death, while chemometric analysis suggested that apoptosis was triggered 48 h after treatment with R-S5-C1-H1. Deconvoluted cellular metabolomics revealed that hydrophobic metabolites were significantly altered, including triacylglycerols, phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, and ceramide, suggesting that apoptosis induction by R-S5-C1-H1 potentially occurred through modulation of phospholipid synthesis and sphingolipid metabolism. These metabolomic profiling results provide new insights into the anticancer mechanisms of E. indica and facilitate the overall understanding of molecular events following therapeutic interventions. Nature Publishing Group 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/33758/2/High%E2%80%90throughput%20metabolomics%20reveals%20dysregulation%20of%20hydrophobic%20metabolomes%20in%20cancer%20cell%20lines%20by%20Eleusine%20indica.pdf text en https://eprints.ums.edu.my/id/eprint/33758/1/High%E2%80%90throughput%20metabolomics%20reveals%20dysregulation%20of%20hydrophobic%20metabolomes%20in%20cancer%20cell%20lines%20by%20Eleusine%20indica%20_ABSTRACT.pdf Perng Yang Puah and Jiunn, Dexter Herng Lee and Soo, Huan Puah and Nik Amin Sahid Nik Lah and Yee, Soon Ling and Fong, Alison Siat Yee (2022) High‐throughput metabolomics reveals dysregulation of hydrophobic metabolomes in cancer cell lines by Eleusine indica. Scientific Reports, 12 (9347). pp. 1-15. ISSN 2045-2322 https://www.nature.com/articles/s41598-022-13575-6.pdf http://dx.doi.org/10.1038/s41598-022-13575-6 http://dx.doi.org/10.1038/s41598-022-13575-6 |
| spellingShingle | RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens RS153-441 Materia medica Perng Yang Puah Jiunn, Dexter Herng Lee Soo, Huan Puah Nik Amin Sahid Nik Lah Yee, Soon Ling Fong, Alison Siat Yee High‐throughput metabolomics reveals dysregulation of hydrophobic metabolomes in cancer cell lines by Eleusine indica |
| title | High‐throughput metabolomics reveals dysregulation of hydrophobic metabolomes in cancer cell lines by Eleusine indica |
| title_full | High‐throughput metabolomics reveals dysregulation of hydrophobic metabolomes in cancer cell lines by Eleusine indica |
| title_fullStr | High‐throughput metabolomics reveals dysregulation of hydrophobic metabolomes in cancer cell lines by Eleusine indica |
| title_full_unstemmed | High‐throughput metabolomics reveals dysregulation of hydrophobic metabolomes in cancer cell lines by Eleusine indica |
| title_short | High‐throughput metabolomics reveals dysregulation of hydrophobic metabolomes in cancer cell lines by Eleusine indica |
| title_sort | high throughput metabolomics reveals dysregulation of hydrophobic metabolomes in cancer cell lines by eleusine indica |
| topic | RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens RS153-441 Materia medica |
| url | https://eprints.ums.edu.my/id/eprint/33758/2/High%E2%80%90throughput%20metabolomics%20reveals%20dysregulation%20of%20hydrophobic%20metabolomes%20in%20cancer%20cell%20lines%20by%20Eleusine%20indica.pdf https://eprints.ums.edu.my/id/eprint/33758/1/High%E2%80%90throughput%20metabolomics%20reveals%20dysregulation%20of%20hydrophobic%20metabolomes%20in%20cancer%20cell%20lines%20by%20Eleusine%20indica%20_ABSTRACT.pdf |
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