Species determination of Malaysian Bactrocera pests using PCR-RFLP analyses (Diptera Tephritidae)
Identification of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, B. tau Walker, B. latifrons Hendel, B. cucurbitae Coquillett, B. umbrosa Fabricius and B. caudata Fabricius would pose a problem if only a body part or an immature stage were available. Analysis of polymerase chai...
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| Format: | Article |
| Language: | English English |
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John Wiley & Sons, Ltd
2010
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| Online Access: | https://eprints.ums.edu.my/id/eprint/33973/1/Species%20determination%20of%20Malaysian%20Bactrocera%20pests%20using%20PCR-RFLP%20analyses%20%28Diptera%20Tephritidae%29.ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/33973/2/Species%20determination%20of%20Malaysian%20Bactrocera%20pests%20using%20PCR-RFLP%20analyses%20%28Diptera%20Tephritidae%29.pdf |
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| author | Tock, Hing Chua Yi, Vern Chong Saw, Hoon Lim |
| author_facet | Tock, Hing Chua Yi, Vern Chong Saw, Hoon Lim |
| author_sort | Tock, Hing Chua |
| collection | UMS |
| description | Identification of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, B. tau Walker, B. latifrons Hendel, B. cucurbitae Coquillett, B. umbrosa Fabricius and B. caudata Fabricius would pose a problem if only a body part or an immature stage were available. Analysis of polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) of cytochrome oxidase I (COI) gene using primers COIR, COIF, UEA7 and UEA10 and restriction enzymes (MseI, RsaI and Alu1) was carried out. The banding profiles in the electrophoresis gel were analysed. The COI gene in six Bactrocera spp. was successfully amplified by COIR and COIF, as well as UEA7 and UEA10, while B. caudata was amplified successfully only by UEA primers. Using COI amplified PCR products and restriction enzymes, distinct banding profiles for B. tau, B. latifrons, B. cucurbitae and B. umbrosa were observed, but not for B. carambolae and B. papayae. However, using UEA7, UEA10 and RsaI, B. caudata could be identified, while B. carambolae and B. papayae might possibly be separated from one another. It was also shown that adult body parts or immature life stages of B. carambolae, B. papayae, B. latifrons and B. cucurbitae produced the same banding profiles as the adults. PCR-RFLP analyses are able to identify positively five Bactrocera species, while B. papayae and B. carambolae might possibly be separated from one another, even if immature life stages or adult body parts are used. |
| first_indexed | 2024-03-06T03:19:42Z |
| format | Article |
| id | ums.eprints-33973 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:19:42Z |
| publishDate | 2010 |
| publisher | John Wiley & Sons, Ltd |
| record_format | dspace |
| spelling | ums.eprints-339732022-08-26T02:26:13Z https://eprints.ums.edu.my/id/eprint/33973/ Species determination of Malaysian Bactrocera pests using PCR-RFLP analyses (Diptera Tephritidae) Tock, Hing Chua Yi, Vern Chong Saw, Hoon Lim QL461-599.82 Insects Identification of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, B. tau Walker, B. latifrons Hendel, B. cucurbitae Coquillett, B. umbrosa Fabricius and B. caudata Fabricius would pose a problem if only a body part or an immature stage were available. Analysis of polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) of cytochrome oxidase I (COI) gene using primers COIR, COIF, UEA7 and UEA10 and restriction enzymes (MseI, RsaI and Alu1) was carried out. The banding profiles in the electrophoresis gel were analysed. The COI gene in six Bactrocera spp. was successfully amplified by COIR and COIF, as well as UEA7 and UEA10, while B. caudata was amplified successfully only by UEA primers. Using COI amplified PCR products and restriction enzymes, distinct banding profiles for B. tau, B. latifrons, B. cucurbitae and B. umbrosa were observed, but not for B. carambolae and B. papayae. However, using UEA7, UEA10 and RsaI, B. caudata could be identified, while B. carambolae and B. papayae might possibly be separated from one another. It was also shown that adult body parts or immature life stages of B. carambolae, B. papayae, B. latifrons and B. cucurbitae produced the same banding profiles as the adults. PCR-RFLP analyses are able to identify positively five Bactrocera species, while B. papayae and B. carambolae might possibly be separated from one another, even if immature life stages or adult body parts are used. John Wiley & Sons, Ltd 2010-04 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/33973/1/Species%20determination%20of%20Malaysian%20Bactrocera%20pests%20using%20PCR-RFLP%20analyses%20%28Diptera%20Tephritidae%29.ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/33973/2/Species%20determination%20of%20Malaysian%20Bactrocera%20pests%20using%20PCR-RFLP%20analyses%20%28Diptera%20Tephritidae%29.pdf Tock, Hing Chua and Yi, Vern Chong and Saw, Hoon Lim (2010) Species determination of Malaysian Bactrocera pests using PCR-RFLP analyses (Diptera Tephritidae). Pest Management Science: formerly Pesticide Science, 66. pp. 379-384. ISSN 1526-4998 https://scholar.google.com/citations?view_op=view_citation&hl=en&user=Zk3YOnsAAAAJ&citft=3&email_for_op=dmasniah%40ums.edu.my&citation_for_view=Zk3YOnsAAAAJ:eQOLeE2rZwMC https://doi.org/10.1002/ps.1886 https://doi.org/10.1002/ps.1886 |
| spellingShingle | QL461-599.82 Insects Tock, Hing Chua Yi, Vern Chong Saw, Hoon Lim Species determination of Malaysian Bactrocera pests using PCR-RFLP analyses (Diptera Tephritidae) |
| title | Species determination of Malaysian Bactrocera pests using PCR-RFLP analyses (Diptera Tephritidae) |
| title_full | Species determination of Malaysian Bactrocera pests using PCR-RFLP analyses (Diptera Tephritidae) |
| title_fullStr | Species determination of Malaysian Bactrocera pests using PCR-RFLP analyses (Diptera Tephritidae) |
| title_full_unstemmed | Species determination of Malaysian Bactrocera pests using PCR-RFLP analyses (Diptera Tephritidae) |
| title_short | Species determination of Malaysian Bactrocera pests using PCR-RFLP analyses (Diptera Tephritidae) |
| title_sort | species determination of malaysian bactrocera pests using pcr rflp analyses diptera tephritidae |
| topic | QL461-599.82 Insects |
| url | https://eprints.ums.edu.my/id/eprint/33973/1/Species%20determination%20of%20Malaysian%20Bactrocera%20pests%20using%20PCR-RFLP%20analyses%20%28Diptera%20Tephritidae%29.ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/33973/2/Species%20determination%20of%20Malaysian%20Bactrocera%20pests%20using%20PCR-RFLP%20analyses%20%28Diptera%20Tephritidae%29.pdf |
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