Electrochemical DNA biosensor for the detection of Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan modified electrode using acridine orange (AO) as a redox indicator
An electrochemical DNA biosensor was developed that is based on a gold electrode modified with a nanocomposite membrane made from an ionic liquid, ZnO nanoparticles and chitosan. A single-stranded DNA probe was immobilized on this electrode. Acridine orange was used as the hybridization probe for mo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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Springer
2011
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| Online Access: | https://eprints.ums.edu.my/id/eprint/34164/1/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/34164/3/ABSTRACT.pdf |
| _version_ | 1825715006242553856 |
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| author | Md. Shafiquzzaman Siddiquee Nor Azah Yusof Abu Bakar Salleh Tan, Soon Guan Fatimah Abu Bakar |
| author_facet | Md. Shafiquzzaman Siddiquee Nor Azah Yusof Abu Bakar Salleh Tan, Soon Guan Fatimah Abu Bakar |
| author_sort | Md. Shafiquzzaman Siddiquee |
| collection | UMS |
| description | An electrochemical DNA biosensor was developed that is based on a gold electrode modified with a nanocomposite membrane made from an ionic liquid, ZnO nanoparticles and chitosan. A single-stranded DNA probe was immobilized on this electrode. Acridine orange was used as the hybridization probe for monitoring the hybridization of the target DNA. The biosensor was capable of detecting target DNA in the concentration range from 1.0 × 10–14 to 1.8 × 10–4 mol L-1, with a detection limit of 1.0 × 10–15 mol L-1. The approach towards constructing a DNA biosensor allows studies on the hybridization even with crude DNA fragments and also to analyze sample obtained from real samples. The results show that the DNA biosensor has the potential for sensitive detection of a specific sequence of the Trichoderma harzianum gene and provides a quick, sensitive and convenient method for the study of microorganisms. |
| first_indexed | 2024-03-06T03:20:14Z |
| format | Article |
| id | ums.eprints-34164 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:20:14Z |
| publishDate | 2011 |
| publisher | Springer |
| record_format | dspace |
| spelling | ums.eprints-341642022-09-27T02:53:07Z https://eprints.ums.edu.my/id/eprint/34164/ Electrochemical DNA biosensor for the detection of Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan modified electrode using acridine orange (AO) as a redox indicator Md. Shafiquzzaman Siddiquee Nor Azah Yusof Abu Bakar Salleh Tan, Soon Guan Fatimah Abu Bakar QD1-999 Chemistry QR1-502 Microbiology An electrochemical DNA biosensor was developed that is based on a gold electrode modified with a nanocomposite membrane made from an ionic liquid, ZnO nanoparticles and chitosan. A single-stranded DNA probe was immobilized on this electrode. Acridine orange was used as the hybridization probe for monitoring the hybridization of the target DNA. The biosensor was capable of detecting target DNA in the concentration range from 1.0 × 10–14 to 1.8 × 10–4 mol L-1, with a detection limit of 1.0 × 10–15 mol L-1. The approach towards constructing a DNA biosensor allows studies on the hybridization even with crude DNA fragments and also to analyze sample obtained from real samples. The results show that the DNA biosensor has the potential for sensitive detection of a specific sequence of the Trichoderma harzianum gene and provides a quick, sensitive and convenient method for the study of microorganisms. Springer 2011 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34164/1/FULL%20TEXT.pdf text en https://eprints.ums.edu.my/id/eprint/34164/3/ABSTRACT.pdf Md. Shafiquzzaman Siddiquee and Nor Azah Yusof and Abu Bakar Salleh and Tan, Soon Guan and Fatimah Abu Bakar (2011) Electrochemical DNA biosensor for the detection of Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan modified electrode using acridine orange (AO) as a redox indicator. Microchimica Acta, 172 (4). pp. 357-363. ISSN 0026-3672 https://link.springer.com/content/pdf/10.1007/s00604-010-0498-7.pdf https://doi.org/10.1007/s00604-010-0498-7 https://doi.org/10.1007/s00604-010-0498-7 |
| spellingShingle | QD1-999 Chemistry QR1-502 Microbiology Md. Shafiquzzaman Siddiquee Nor Azah Yusof Abu Bakar Salleh Tan, Soon Guan Fatimah Abu Bakar Electrochemical DNA biosensor for the detection of Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan modified electrode using acridine orange (AO) as a redox indicator |
| title | Electrochemical DNA biosensor for the detection of Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan modified electrode using acridine orange (AO) as a redox indicator |
| title_full | Electrochemical DNA biosensor for the detection of Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan modified electrode using acridine orange (AO) as a redox indicator |
| title_fullStr | Electrochemical DNA biosensor for the detection of Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan modified electrode using acridine orange (AO) as a redox indicator |
| title_full_unstemmed | Electrochemical DNA biosensor for the detection of Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan modified electrode using acridine orange (AO) as a redox indicator |
| title_short | Electrochemical DNA biosensor for the detection of Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan modified electrode using acridine orange (AO) as a redox indicator |
| title_sort | electrochemical dna biosensor for the detection of trichoderma harzianum based on ionic liquid zno nanoparticles chitosan modified electrode using acridine orange ao as a redox indicator |
| topic | QD1-999 Chemistry QR1-502 Microbiology |
| url | https://eprints.ums.edu.my/id/eprint/34164/1/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/34164/3/ABSTRACT.pdf |
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