The possibility of a halal mix probiotic medium for the cultivation of Lactobacillus plantarum N16 and Saccharomyces cerevisiae

This study aimed to determine the effects of interaction between media type (halal mix preparation) and culture mixtures of Lactobacillus plantarum N16 and Saccharomyces cerevisiae (probiotics). A completely randomized factorial design (CRFD) consisting of 2 factors and three replications was used,...

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Main Authors: Yetti Marlida, Harnentis, Azizah, Yuliaty Shafan Nur, Frederick Adzitey, Norliza Julmohammad, Nurul Huda
Format: Article
Language:English
English
Published: Public Knowledge Project 2022
Subjects:
Online Access:https://eprints.ums.edu.my/id/eprint/34348/1/Abstract.pdf
https://eprints.ums.edu.my/id/eprint/34348/2/Full%20text.pdf
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author Yetti Marlida
Harnentis
Azizah
Yuliaty Shafan Nur
Frederick Adzitey
Norliza Julmohammad
Nurul Huda
author_facet Yetti Marlida
Harnentis
Azizah
Yuliaty Shafan Nur
Frederick Adzitey
Norliza Julmohammad
Nurul Huda
author_sort Yetti Marlida
collection UMS
description This study aimed to determine the effects of interaction between media type (halal mix preparation) and culture mixtures of Lactobacillus plantarum N16 and Saccharomyces cerevisiae (probiotics). A completely randomized factorial design (CRFD) consisting of 2 factors and three replications was used, where factor A was a mixture of Lactobacillus plantarum N16 and Saccharomyces cerevisiae at a ratio of 1:1 (A1); 1:2 (A2) and 2:1 (A3) and factor B was the type of growth media, that is, control (B1), whey tofu, molasses, and fish waste flour (B2), and coconut water, onggok flour and shrimp waste flour (B3). The variables measured were viability, cell biomass, and pH. The results showed interactions between factors A and B, which were significantly different (p <0.05) in terms of viability, cell biomass, and pH. Based on the results of the study, it can be concluded that the mixture of Lactobacillus plantarum N16 and Saccharomyces cerevisiae at a ratio of 2:1 (A3), using coconut water, onggok flour, and shrimp waste flour (B3) as medium and incubated at 36 °C for 24 hours was the best medium. It had a 2.37 viability, 42.33 mg/ml biomass cell, and a pH of 2.37.
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spelling ums.eprints-343482022-09-29T03:50:54Z https://eprints.ums.edu.my/id/eprint/34348/ The possibility of a halal mix probiotic medium for the cultivation of Lactobacillus plantarum N16 and Saccharomyces cerevisiae Yetti Marlida Harnentis Azizah Yuliaty Shafan Nur Frederick Adzitey Norliza Julmohammad Nurul Huda BP174-190 The practice of Islam TX341-641 Nutrition. Foods and food supply This study aimed to determine the effects of interaction between media type (halal mix preparation) and culture mixtures of Lactobacillus plantarum N16 and Saccharomyces cerevisiae (probiotics). A completely randomized factorial design (CRFD) consisting of 2 factors and three replications was used, where factor A was a mixture of Lactobacillus plantarum N16 and Saccharomyces cerevisiae at a ratio of 1:1 (A1); 1:2 (A2) and 2:1 (A3) and factor B was the type of growth media, that is, control (B1), whey tofu, molasses, and fish waste flour (B2), and coconut water, onggok flour and shrimp waste flour (B3). The variables measured were viability, cell biomass, and pH. The results showed interactions between factors A and B, which were significantly different (p <0.05) in terms of viability, cell biomass, and pH. Based on the results of the study, it can be concluded that the mixture of Lactobacillus plantarum N16 and Saccharomyces cerevisiae at a ratio of 2:1 (A3), using coconut water, onggok flour, and shrimp waste flour (B3) as medium and incubated at 36 °C for 24 hours was the best medium. It had a 2.37 viability, 42.33 mg/ml biomass cell, and a pH of 2.37. Public Knowledge Project 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34348/1/Abstract.pdf text en https://eprints.ums.edu.my/id/eprint/34348/2/Full%20text.pdf Yetti Marlida and Harnentis and Azizah and Yuliaty Shafan Nur and Frederick Adzitey and Norliza Julmohammad and Nurul Huda (2022) The possibility of a halal mix probiotic medium for the cultivation of Lactobacillus plantarum N16 and Saccharomyces cerevisiae. Potravinarstvo Slovak Journal of Food Sciences, 16. pp. 279-286. ISSN 1337-0960 https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1713#:~:text=Based%20on%20the%20results%20of,hours%20was%20the%20best%20medium. https://doi.org/10.5219/1713 https://doi.org/10.5219/1713
spellingShingle BP174-190 The practice of Islam
TX341-641 Nutrition. Foods and food supply
Yetti Marlida
Harnentis
Azizah
Yuliaty Shafan Nur
Frederick Adzitey
Norliza Julmohammad
Nurul Huda
The possibility of a halal mix probiotic medium for the cultivation of Lactobacillus plantarum N16 and Saccharomyces cerevisiae
title The possibility of a halal mix probiotic medium for the cultivation of Lactobacillus plantarum N16 and Saccharomyces cerevisiae
title_full The possibility of a halal mix probiotic medium for the cultivation of Lactobacillus plantarum N16 and Saccharomyces cerevisiae
title_fullStr The possibility of a halal mix probiotic medium for the cultivation of Lactobacillus plantarum N16 and Saccharomyces cerevisiae
title_full_unstemmed The possibility of a halal mix probiotic medium for the cultivation of Lactobacillus plantarum N16 and Saccharomyces cerevisiae
title_short The possibility of a halal mix probiotic medium for the cultivation of Lactobacillus plantarum N16 and Saccharomyces cerevisiae
title_sort possibility of a halal mix probiotic medium for the cultivation of lactobacillus plantarum n16 and saccharomyces cerevisiae
topic BP174-190 The practice of Islam
TX341-641 Nutrition. Foods and food supply
url https://eprints.ums.edu.my/id/eprint/34348/1/Abstract.pdf
https://eprints.ums.edu.my/id/eprint/34348/2/Full%20text.pdf
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