Biochemical and Microstructural Properties of Lizardfish (Saurida tumbil) Scale Collagen Extracted with Various Organic Acids
The purpose of this research was to extract collagen from the scales of lizardfish (Sauridatumbil) using various acids. Acetic acid-extracted collagen (AScC) produced a higher yield (1.8 mg/g)than lactic acid-extracted collagen (LScC) and citric acid-extracted collagen (CScC) although not significan...
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| Format: | Article |
| Language: | English English |
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Multidisciplinary Digital Publishing Institute (MDPI)
2022
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| Online Access: | https://eprints.ums.edu.my/id/eprint/34454/1/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/34454/2/ABSTRACT.pdf |
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| author | Abdul Aziz Jaziri Rossita Shapawi Ruzaidi Azli Mohd Mokhtar Wan Norhana Md. Noordin Nurul Huda |
| author_facet | Abdul Aziz Jaziri Rossita Shapawi Ruzaidi Azli Mohd Mokhtar Wan Norhana Md. Noordin Nurul Huda |
| author_sort | Abdul Aziz Jaziri |
| collection | UMS |
| description | The purpose of this research was to extract collagen from the scales of lizardfish (Sauridatumbil) using various acids. Acetic acid-extracted collagen (AScC) produced a higher yield (1.8 mg/g)than lactic acid-extracted collagen (LScC) and citric acid-extracted collagen (CScC) although not significantly different (p> 0.05). All extracted collagens were categorized as type I collagens with the presence of alpha chains (α1 and α2) based on the SDS-PAGE profiles. The triple-helical structure of the collagen was maintained in the AScC, LScC, and CScC as confirmed by the FTIR spectra. The UV-vis and X-ray diffraction spectra observed in all collagens were in agreement with previous work on fish scale and calfskin (commercial) collagens. The thermal stability of AScC (Tmax= 31.61◦C) was greater than LScC (Tmax= 30.86◦C) and CScC (Tmax= 30.88◦C). The microstructure of acid-extracted collagens was characterized as complex, fibrous, and multilayered, with irregular sheet-like structures.All samples were highly soluble in acidic pH (1.0–4.0) and in low concentrations of NaCl (0–20 g/L).In conclusion, the lizardfish scale collagen, particularly AScC, may be used as an alternative to terrestrial animal collagen. |
| first_indexed | 2024-03-06T03:20:59Z |
| format | Article |
| id | ums.eprints-34454 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:20:59Z |
| publishDate | 2022 |
| publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
| record_format | dspace |
| spelling | ums.eprints-344542022-10-18T06:04:27Z https://eprints.ums.edu.my/id/eprint/34454/ Biochemical and Microstructural Properties of Lizardfish (Saurida tumbil) Scale Collagen Extracted with Various Organic Acids Abdul Aziz Jaziri Rossita Shapawi Ruzaidi Azli Mohd Mokhtar Wan Norhana Md. Noordin Nurul Huda QP501-801 Animal biochemistry The purpose of this research was to extract collagen from the scales of lizardfish (Sauridatumbil) using various acids. Acetic acid-extracted collagen (AScC) produced a higher yield (1.8 mg/g)than lactic acid-extracted collagen (LScC) and citric acid-extracted collagen (CScC) although not significantly different (p> 0.05). All extracted collagens were categorized as type I collagens with the presence of alpha chains (α1 and α2) based on the SDS-PAGE profiles. The triple-helical structure of the collagen was maintained in the AScC, LScC, and CScC as confirmed by the FTIR spectra. The UV-vis and X-ray diffraction spectra observed in all collagens were in agreement with previous work on fish scale and calfskin (commercial) collagens. The thermal stability of AScC (Tmax= 31.61◦C) was greater than LScC (Tmax= 30.86◦C) and CScC (Tmax= 30.88◦C). The microstructure of acid-extracted collagens was characterized as complex, fibrous, and multilayered, with irregular sheet-like structures.All samples were highly soluble in acidic pH (1.0–4.0) and in low concentrations of NaCl (0–20 g/L).In conclusion, the lizardfish scale collagen, particularly AScC, may be used as an alternative to terrestrial animal collagen. Multidisciplinary Digital Publishing Institute (MDPI) 2022 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34454/1/FULL%20TEXT.pdf text en https://eprints.ums.edu.my/id/eprint/34454/2/ABSTRACT.pdf Abdul Aziz Jaziri and Rossita Shapawi and Ruzaidi Azli Mohd Mokhtar and Wan Norhana Md. Noordin and Nurul Huda (2022) Biochemical and Microstructural Properties of Lizardfish (Saurida tumbil) Scale Collagen Extracted with Various Organic Acids. Gels, 8 (266). pp. 1-18. ISSN 2310-2861 https://www.mdpi.com/2310-2861/8/5/266/htm https://doi.org/10.3390/gels8050266 https://doi.org/10.3390/gels8050266 |
| spellingShingle | QP501-801 Animal biochemistry Abdul Aziz Jaziri Rossita Shapawi Ruzaidi Azli Mohd Mokhtar Wan Norhana Md. Noordin Nurul Huda Biochemical and Microstructural Properties of Lizardfish (Saurida tumbil) Scale Collagen Extracted with Various Organic Acids |
| title | Biochemical and Microstructural Properties of Lizardfish (Saurida tumbil) Scale Collagen Extracted with Various Organic Acids |
| title_full | Biochemical and Microstructural Properties of Lizardfish (Saurida tumbil) Scale Collagen Extracted with Various Organic Acids |
| title_fullStr | Biochemical and Microstructural Properties of Lizardfish (Saurida tumbil) Scale Collagen Extracted with Various Organic Acids |
| title_full_unstemmed | Biochemical and Microstructural Properties of Lizardfish (Saurida tumbil) Scale Collagen Extracted with Various Organic Acids |
| title_short | Biochemical and Microstructural Properties of Lizardfish (Saurida tumbil) Scale Collagen Extracted with Various Organic Acids |
| title_sort | biochemical and microstructural properties of lizardfish saurida tumbil scale collagen extracted with various organic acids |
| topic | QP501-801 Animal biochemistry |
| url | https://eprints.ums.edu.my/id/eprint/34454/1/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/34454/2/ABSTRACT.pdf |
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