Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation
Curcumin is an active compound derived from turmeric, Curcuma longa, and is known for its benefits to human health. The amoebicidal activity of curcumin against Acanthamoeba triangularis was recently discovered. However, a physiological change of intracellular pathways related to A. triangularis enc...
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| Format: | Article |
| Language: | English English |
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Boonhok et al.
2022
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| Online Access: | https://eprints.ums.edu.my/id/eprint/34519/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/34519/2/FULLTEXT.pdf |
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| author | Rachasak Boonhok Suthinee Sangkanu Suganya Phumjan Ramita Jongboonjua Nawarat Sangnopparat Pattamaporn Kwankaew Aman Tedasen Lim, Chooi Ling Maria de Lourdes Pereira Mohammed Rahmatullah Polrat Wilairatana Christophe Wiart Karma G. Dolma Alok K. Paul Madhu Gupta Veeranoot Nissapatorn |
| author_facet | Rachasak Boonhok Suthinee Sangkanu Suganya Phumjan Ramita Jongboonjua Nawarat Sangnopparat Pattamaporn Kwankaew Aman Tedasen Lim, Chooi Ling Maria de Lourdes Pereira Mohammed Rahmatullah Polrat Wilairatana Christophe Wiart Karma G. Dolma Alok K. Paul Madhu Gupta Veeranoot Nissapatorn |
| author_sort | Rachasak Boonhok |
| collection | UMS |
| description | Curcumin is an active compound derived from turmeric, Curcuma longa, and is known for its benefits to human health. The amoebicidal activity of curcumin against Acanthamoeba triangularis was recently discovered. However, a physiological change of intracellular pathways related to A. triangularis encystation mechanism, including autophagy in the surviving amoeba after curcumin treatment, has never been reported. This study aims to investigate the effect of curcumin on the survival of A. triangularis under nutrient starvation and nutrient-rich condition, as well as to evaluate the A. triangularis encystation and a physiological change of Acanthamoeba autophagy at the mRNA level. In this study, A. triangularis amoebas were treated with a sublethal dose of curcumin under nutrient starvation and nutrient-rich condition and the surviving amoebas was investigated. Cysts formation and vacuolization were examined by microscopy and transcriptional expression of autophagy-related genes and other encystation-related genes were evaluated by real-time PCR. A. triangularis cysts were formed under nutrient starvation. However, in the presence of the autophagy inhibitor, 3-methyladenine (3-MA), the percentage of cysts was significantly reduced. Interestingly, in the presence of curcumin, most of the parasites remained in the trophozoite stage in both the starvation and nutrient-rich condition. In vacuolization analysis, the percentage of amoebas with enlarged vacuole was increased upon starvation. However, the percentage was significantly declined in the presence of curcumin and 3-MA. Molecular analysis of A. triangularis autophagy-related (ATG) genes showed that the mRNA expression of the ATG genes, ATG3, ATG8b, ATG12, ATG16, under the starvation with curcumin was at a basal level along the treatment. The results were similar to those of the curcumin-treated amoebas under a nutrient-rich condition, except AcATG16 which increased later. On the other hand, mRNA expression of encystation-related genes, cellulose synthase and serine proteinase, remained unchanged during the first 18 h, but significantly increased at 24 h post treatment. Curcumin inhibits cyst formation in surviving trophozoites, which may result from its effect on mRNA expression of key Acanthamoeba ATG-related genes. However, further investigation into the mechanism of curcumin in A. triangularis trophozoites arrest and its association with autophagy or other encystation-related pathways is needed to support the future use of curcumin. |
| first_indexed | 2024-03-06T03:21:11Z |
| format | Article |
| id | ums.eprints-34519 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:21:11Z |
| publishDate | 2022 |
| publisher | Boonhok et al. |
| record_format | dspace |
| spelling | ums.eprints-345192022-10-27T02:01:21Z https://eprints.ums.edu.my/id/eprint/34519/ Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation Rachasak Boonhok Suthinee Sangkanu Suganya Phumjan Ramita Jongboonjua Nawarat Sangnopparat Pattamaporn Kwankaew Aman Tedasen Lim, Chooi Ling Maria de Lourdes Pereira Mohammed Rahmatullah Polrat Wilairatana Christophe Wiart Karma G. Dolma Alok K. Paul Madhu Gupta Veeranoot Nissapatorn TP1-1185 Chemical technology Curcumin is an active compound derived from turmeric, Curcuma longa, and is known for its benefits to human health. The amoebicidal activity of curcumin against Acanthamoeba triangularis was recently discovered. However, a physiological change of intracellular pathways related to A. triangularis encystation mechanism, including autophagy in the surviving amoeba after curcumin treatment, has never been reported. This study aims to investigate the effect of curcumin on the survival of A. triangularis under nutrient starvation and nutrient-rich condition, as well as to evaluate the A. triangularis encystation and a physiological change of Acanthamoeba autophagy at the mRNA level. In this study, A. triangularis amoebas were treated with a sublethal dose of curcumin under nutrient starvation and nutrient-rich condition and the surviving amoebas was investigated. Cysts formation and vacuolization were examined by microscopy and transcriptional expression of autophagy-related genes and other encystation-related genes were evaluated by real-time PCR. A. triangularis cysts were formed under nutrient starvation. However, in the presence of the autophagy inhibitor, 3-methyladenine (3-MA), the percentage of cysts was significantly reduced. Interestingly, in the presence of curcumin, most of the parasites remained in the trophozoite stage in both the starvation and nutrient-rich condition. In vacuolization analysis, the percentage of amoebas with enlarged vacuole was increased upon starvation. However, the percentage was significantly declined in the presence of curcumin and 3-MA. Molecular analysis of A. triangularis autophagy-related (ATG) genes showed that the mRNA expression of the ATG genes, ATG3, ATG8b, ATG12, ATG16, under the starvation with curcumin was at a basal level along the treatment. The results were similar to those of the curcumin-treated amoebas under a nutrient-rich condition, except AcATG16 which increased later. On the other hand, mRNA expression of encystation-related genes, cellulose synthase and serine proteinase, remained unchanged during the first 18 h, but significantly increased at 24 h post treatment. Curcumin inhibits cyst formation in surviving trophozoites, which may result from its effect on mRNA expression of key Acanthamoeba ATG-related genes. However, further investigation into the mechanism of curcumin in A. triangularis trophozoites arrest and its association with autophagy or other encystation-related pathways is needed to support the future use of curcumin. Boonhok et al. 2022-07-04 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34519/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/34519/2/FULLTEXT.pdf Rachasak Boonhok and Suthinee Sangkanu and Suganya Phumjan and Ramita Jongboonjua and Nawarat Sangnopparat and Pattamaporn Kwankaew and Aman Tedasen and Lim, Chooi Ling and Maria de Lourdes Pereira and Mohammed Rahmatullah and Polrat Wilairatana and Christophe Wiart and Karma G. Dolma and Alok K. Paul and Madhu Gupta and Veeranoot Nissapatorn (2022) Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation. Biochemistry, Biophysics And Molecular Biology, 10. pp. 1-27. https://peerj.com/articles/13657/ https://doi.org/10.7717/peerj.13657 https://doi.org/10.7717/peerj.13657 |
| spellingShingle | TP1-1185 Chemical technology Rachasak Boonhok Suthinee Sangkanu Suganya Phumjan Ramita Jongboonjua Nawarat Sangnopparat Pattamaporn Kwankaew Aman Tedasen Lim, Chooi Ling Maria de Lourdes Pereira Mohammed Rahmatullah Polrat Wilairatana Christophe Wiart Karma G. Dolma Alok K. Paul Madhu Gupta Veeranoot Nissapatorn Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation |
| title | Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation |
| title_full | Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation |
| title_fullStr | Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation |
| title_full_unstemmed | Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation |
| title_short | Curcumin effect on Acanthamoeba triangularis encystation under nutrient starvation |
| title_sort | curcumin effect on acanthamoeba triangularis encystation under nutrient starvation |
| topic | TP1-1185 Chemical technology |
| url | https://eprints.ums.edu.my/id/eprint/34519/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/34519/2/FULLTEXT.pdf |
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