Evaluation of 16S rRNA methyltransferase gene in aminoglycosides resistant isolates of cancer patients
Aminoglycosides are used in empiric treatment of critically ill patients. Efficacy of aminoglycoside has been reduced due to dissemination of resistance. The aim of this study was to evaluate aminoglycoside resistance in cancer patients with pneumoniae. A total of 150 Bronchoalveolar lavage and Bron...
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| Formatua: | Artikulua |
| Hizkuntza: | English English |
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University of the Central Punjab
2021
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| Sarrera elektronikoa: | https://eprints.ums.edu.my/id/eprint/34814/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34814/2/Full%20text.pdf |
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| author | Saba Ghafoor Basit Zeshan Madiha Khan Saba Kabir |
| author_facet | Saba Ghafoor Basit Zeshan Madiha Khan Saba Kabir |
| author_sort | Saba Ghafoor |
| collection | UMS |
| description | Aminoglycosides are used in empiric treatment of critically ill patients. Efficacy of aminoglycoside has been reduced due to dissemination of resistance. The aim of this study was to evaluate aminoglycoside resistance in cancer patients with pneumoniae. A total of 150 Bronchoalveolar lavage and Bronchial washing samples were collected from cancer patients. The samples were identified with standard microbiological procedures. Phenotypic susceptibility pattern of the isolates was determined against various groups of antibiotics such as Penicillins, Cephalosporins, Carbapenems, Monobactams, Aminoglycosides, Tetracyclins, Glycopeptides and Sulphonamides. The isolates with phenotypic resistant to aminoglycosides were further evaluated for the presence of armA gene. The strains of E. coli (12.5%), S. aureus (15.6%), Streptococcus (15.6%), Pseudomonas (18.7%) and K. pneumoniae (37.5%) were isolated. The phenotypic resistance profile showed highest resistance against aminoglycosides (Tobramycin, 53.1% Gentamicin and 50% Amikacin) followed by cephalosporins and sulfonamides group. The armA gene was detected in aminoglycoside resistant isolates. The overall genotypic resistance was evaluated as 21.8%. The armA gene was found in K.pneumoniae 23.5%, Pseudomonas 11.8% (4/24) and E. coli 5.9%. High level resistance to aminoglycosides raises therapeutic concern to health care professionals. These findings highlight the importance of effective monitoring and surveillance to the use of broad-spectrum antibiotics. |
| first_indexed | 2024-03-06T03:22:01Z |
| format | Article |
| id | ums.eprints-34814 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:22:01Z |
| publishDate | 2021 |
| publisher | University of the Central Punjab |
| record_format | dspace |
| spelling | ums.eprints-348142022-11-10T13:17:53Z https://eprints.ums.edu.my/id/eprint/34814/ Evaluation of 16S rRNA methyltransferase gene in aminoglycosides resistant isolates of cancer patients Saba Ghafoor Basit Zeshan Madiha Khan Saba Kabir QR1-502 Microbiology Aminoglycosides are used in empiric treatment of critically ill patients. Efficacy of aminoglycoside has been reduced due to dissemination of resistance. The aim of this study was to evaluate aminoglycoside resistance in cancer patients with pneumoniae. A total of 150 Bronchoalveolar lavage and Bronchial washing samples were collected from cancer patients. The samples were identified with standard microbiological procedures. Phenotypic susceptibility pattern of the isolates was determined against various groups of antibiotics such as Penicillins, Cephalosporins, Carbapenems, Monobactams, Aminoglycosides, Tetracyclins, Glycopeptides and Sulphonamides. The isolates with phenotypic resistant to aminoglycosides were further evaluated for the presence of armA gene. The strains of E. coli (12.5%), S. aureus (15.6%), Streptococcus (15.6%), Pseudomonas (18.7%) and K. pneumoniae (37.5%) were isolated. The phenotypic resistance profile showed highest resistance against aminoglycosides (Tobramycin, 53.1% Gentamicin and 50% Amikacin) followed by cephalosporins and sulfonamides group. The armA gene was detected in aminoglycoside resistant isolates. The overall genotypic resistance was evaluated as 21.8%. The armA gene was found in K.pneumoniae 23.5%, Pseudomonas 11.8% (4/24) and E. coli 5.9%. High level resistance to aminoglycosides raises therapeutic concern to health care professionals. These findings highlight the importance of effective monitoring and surveillance to the use of broad-spectrum antibiotics. University of the Central Punjab 2021 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34814/1/Abstract.pdf text en https://eprints.ums.edu.my/id/eprint/34814/2/Full%20text.pdf Saba Ghafoor and Basit Zeshan and Madiha Khan and Saba Kabir (2021) Evaluation of 16S rRNA methyltransferase gene in aminoglycosides resistant isolates of cancer patients. Pakistan Journal of Pharmaceutical Sciences, 34. pp. 1737-1741. ISSN 1011-601X http://www.pjps.pk/wp-content/uploads/pdfs/34/5/Paper-14.pdf https://doi.org/10.36721/PJPS.2021.34.5.REG.1737-1741.1 https://doi.org/10.36721/PJPS.2021.34.5.REG.1737-1741.1 |
| spellingShingle | QR1-502 Microbiology Saba Ghafoor Basit Zeshan Madiha Khan Saba Kabir Evaluation of 16S rRNA methyltransferase gene in aminoglycosides resistant isolates of cancer patients |
| title | Evaluation of 16S rRNA methyltransferase gene in aminoglycosides resistant isolates of cancer patients |
| title_full | Evaluation of 16S rRNA methyltransferase gene in aminoglycosides resistant isolates of cancer patients |
| title_fullStr | Evaluation of 16S rRNA methyltransferase gene in aminoglycosides resistant isolates of cancer patients |
| title_full_unstemmed | Evaluation of 16S rRNA methyltransferase gene in aminoglycosides resistant isolates of cancer patients |
| title_short | Evaluation of 16S rRNA methyltransferase gene in aminoglycosides resistant isolates of cancer patients |
| title_sort | evaluation of 16s rrna methyltransferase gene in aminoglycosides resistant isolates of cancer patients |
| topic | QR1-502 Microbiology |
| url | https://eprints.ums.edu.my/id/eprint/34814/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34814/2/Full%20text.pdf |
| work_keys_str_mv | AT sabaghafoor evaluationof16srrnamethyltransferasegeneinaminoglycosidesresistantisolatesofcancerpatients AT basitzeshan evaluationof16srrnamethyltransferasegeneinaminoglycosidesresistantisolatesofcancerpatients AT madihakhan evaluationof16srrnamethyltransferasegeneinaminoglycosidesresistantisolatesofcancerpatients AT sabakabir evaluationof16srrnamethyltransferasegeneinaminoglycosidesresistantisolatesofcancerpatients |