Assessing seroprevalence and associated risk factors for multiple infectious diseases in Sabah, Malaysia using serological multiplex bead assays
Infectious diseases continue to burden populations in Malaysia, especially among rural communities where resources are limited and access to health care is difficult. Current epidemiological trends of several neglected tropical diseases in these populations are at present absent due to the lack of h...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Frontiers Media S.A.
2022
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| Online Access: | https://eprints.ums.edu.my/id/eprint/34831/1/ABSTRAK.pdf https://eprints.ums.edu.my/id/eprint/34831/2/FULLTEXT.pdf |
| _version_ | 1796911666435194880 |
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| author | Chan, YuYen L. Catriona L. Patterson Jeffrey W. Priest Gillian Stresman Timothy William Chua, Tock Hing Kevin Tetteh Patrick Lammie Chris Drakeley Kimberly M. Fornace |
| author_facet | Chan, YuYen L. Catriona L. Patterson Jeffrey W. Priest Gillian Stresman Timothy William Chua, Tock Hing Kevin Tetteh Patrick Lammie Chris Drakeley Kimberly M. Fornace |
| author_sort | Chan, YuYen L. |
| collection | UMS |
| description | Infectious diseases continue to burden populations in Malaysia, especially among rural communities where resources are limited and access to health care is difficult. Current epidemiological trends of several neglected tropical diseases in these populations are at present absent due to the lack of habitual and efficient surveillance. To date, various studies have explored the utility of serological multiplex beads to monitor numerous diseases simultaneously. We therefore applied this platform to assess population level exposure to six infectious diseases in Sabah, Malaysia. Furthermore, we concurrently investigated demographic and spatial risk factors that may be associated with exposure for each disease. This study was conducted in four districts of Northern Sabah in Malaysian Borneo, using an environmentally stratified, population-based cross-sectional serological survey targeted to determine risk factors for malaria. Samples were collected between September to December 2015, from 919 villages totaling 10,100 persons. IgG responses to twelve antigens of six diseases (lymphatic filariasis- Bm33, Bm14, BmR1, Wb123; strongyloides- NIE; toxoplasmosis-SAG2A; yaws- Rp17 and TmpA; trachoma- Pgp3, Ct694; and giardiasis- VSP3, VSP5) were measured using serological multiplex bead assays. Eight demographic risk factors and twelve environmental covariates were included in this study to better understand transmission in this community. Seroprevalence of LF antigens included Bm33 (10.9%), Bm14+ BmR1 (3.5%), and Wb123 (1.7%). Seroprevalence of Strongyloides antigen NIE was 16.8%, for Toxoplasma antigen SAG2A was 29.9%, and Giardia antigens GVSP3 + GVSP5 was 23.2%. Seroprevalence estimates for yaws Rp17 was 4.91%, for TmpA was 4.81%, and for combined seropositivity to both antigens was 1.2%. Seroprevalence estimates for trachoma Pgp3 + Ct694 were 4.5%. Age was a significant risk factors consistent among all antigens assessed, while other risk factors varied among the different antigens. Spatial heterogeneity of seroprevalence was observed more prominently in lymphatic filariasis and toxoplasmosis. Multiplex bead assays can be used to assess serological responses to numerous pathogens simultaneously to support infectious disease surveillance in rural communities, especially where prevalences estimates are lacking for neglected tropical diseases. Demographic and spatial data collected alongside serosurveys can prove useful in identifying risk factors associated with exposure and geographic distribution of transmission. |
| first_indexed | 2024-03-06T03:22:04Z |
| format | Article |
| id | ums.eprints-34831 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:22:04Z |
| publishDate | 2022 |
| publisher | Frontiers Media S.A. |
| record_format | dspace |
| spelling | ums.eprints-348312022-11-10T13:48:46Z https://eprints.ums.edu.my/id/eprint/34831/ Assessing seroprevalence and associated risk factors for multiple infectious diseases in Sabah, Malaysia using serological multiplex bead assays Chan, YuYen L. Catriona L. Patterson Jeffrey W. Priest Gillian Stresman Timothy William Chua, Tock Hing Kevin Tetteh Patrick Lammie Chris Drakeley Kimberly M. Fornace RA639-642 Transmission of disease Infectious diseases continue to burden populations in Malaysia, especially among rural communities where resources are limited and access to health care is difficult. Current epidemiological trends of several neglected tropical diseases in these populations are at present absent due to the lack of habitual and efficient surveillance. To date, various studies have explored the utility of serological multiplex beads to monitor numerous diseases simultaneously. We therefore applied this platform to assess population level exposure to six infectious diseases in Sabah, Malaysia. Furthermore, we concurrently investigated demographic and spatial risk factors that may be associated with exposure for each disease. This study was conducted in four districts of Northern Sabah in Malaysian Borneo, using an environmentally stratified, population-based cross-sectional serological survey targeted to determine risk factors for malaria. Samples were collected between September to December 2015, from 919 villages totaling 10,100 persons. IgG responses to twelve antigens of six diseases (lymphatic filariasis- Bm33, Bm14, BmR1, Wb123; strongyloides- NIE; toxoplasmosis-SAG2A; yaws- Rp17 and TmpA; trachoma- Pgp3, Ct694; and giardiasis- VSP3, VSP5) were measured using serological multiplex bead assays. Eight demographic risk factors and twelve environmental covariates were included in this study to better understand transmission in this community. Seroprevalence of LF antigens included Bm33 (10.9%), Bm14+ BmR1 (3.5%), and Wb123 (1.7%). Seroprevalence of Strongyloides antigen NIE was 16.8%, for Toxoplasma antigen SAG2A was 29.9%, and Giardia antigens GVSP3 + GVSP5 was 23.2%. Seroprevalence estimates for yaws Rp17 was 4.91%, for TmpA was 4.81%, and for combined seropositivity to both antigens was 1.2%. Seroprevalence estimates for trachoma Pgp3 + Ct694 were 4.5%. Age was a significant risk factors consistent among all antigens assessed, while other risk factors varied among the different antigens. Spatial heterogeneity of seroprevalence was observed more prominently in lymphatic filariasis and toxoplasmosis. Multiplex bead assays can be used to assess serological responses to numerous pathogens simultaneously to support infectious disease surveillance in rural communities, especially where prevalences estimates are lacking for neglected tropical diseases. Demographic and spatial data collected alongside serosurveys can prove useful in identifying risk factors associated with exposure and geographic distribution of transmission. Frontiers Media S.A. 2022-10-25 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34831/1/ABSTRAK.pdf text en https://eprints.ums.edu.my/id/eprint/34831/2/FULLTEXT.pdf Chan, YuYen L. and Catriona L. Patterson and Jeffrey W. Priest and Gillian Stresman and Timothy William and Chua, Tock Hing and Kevin Tetteh and Patrick Lammie and Chris Drakeley and Kimberly M. Fornace (2022) Assessing seroprevalence and associated risk factors for multiple infectious diseases in Sabah, Malaysia using serological multiplex bead assays. Frontiers in Public Health. pp. 1-11. ISSN 2296-2565 https://www.frontiersin.org/articles/10.3389/fpubh.2022.924316/full https://doi.org/10.3389/fpubh.2022.924316 https://doi.org/10.3389/fpubh.2022.924316 |
| spellingShingle | RA639-642 Transmission of disease Chan, YuYen L. Catriona L. Patterson Jeffrey W. Priest Gillian Stresman Timothy William Chua, Tock Hing Kevin Tetteh Patrick Lammie Chris Drakeley Kimberly M. Fornace Assessing seroprevalence and associated risk factors for multiple infectious diseases in Sabah, Malaysia using serological multiplex bead assays |
| title | Assessing seroprevalence and associated risk factors for multiple infectious diseases in Sabah, Malaysia using serological multiplex bead assays |
| title_full | Assessing seroprevalence and associated risk factors for multiple infectious diseases in Sabah, Malaysia using serological multiplex bead assays |
| title_fullStr | Assessing seroprevalence and associated risk factors for multiple infectious diseases in Sabah, Malaysia using serological multiplex bead assays |
| title_full_unstemmed | Assessing seroprevalence and associated risk factors for multiple infectious diseases in Sabah, Malaysia using serological multiplex bead assays |
| title_short | Assessing seroprevalence and associated risk factors for multiple infectious diseases in Sabah, Malaysia using serological multiplex bead assays |
| title_sort | assessing seroprevalence and associated risk factors for multiple infectious diseases in sabah malaysia using serological multiplex bead assays |
| topic | RA639-642 Transmission of disease |
| url | https://eprints.ums.edu.my/id/eprint/34831/1/ABSTRAK.pdf https://eprints.ums.edu.my/id/eprint/34831/2/FULLTEXT.pdf |
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