Study of Gc-ms And Hplc Characterized Metabolic Compounds in Guava (Psidium Guajava L.) Leaves
Psidium guajava leaves are rich source of nutrients, antioxidants, phytoconstituents and biological active compounds. The study was designed to elucidate secondary metabolites like alkaloids, saponins, flavonoids, tannins and glycosides in extracts of guava leaves through Gas chromatography-mass spe...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Pakistan Journal of Agricultural Sciences
2019
|
| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/34842/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34842/2/Full%20text.pdf |
| _version_ | 1825715127770415104 |
|---|---|
| author | Muhammad Afzal Riffat Iqbal Zahed Mahmood Basit Zeshan Javed Iqbal Wattoo |
| author_facet | Muhammad Afzal Riffat Iqbal Zahed Mahmood Basit Zeshan Javed Iqbal Wattoo |
| author_sort | Muhammad Afzal |
| collection | UMS |
| description | Psidium guajava leaves are rich source of nutrients, antioxidants, phytoconstituents and biological active compounds. The study was designed to elucidate secondary metabolites like alkaloids, saponins, flavonoids, tannins and glycosides in extracts of guava leaves through Gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) by qualitative as well as quantitative procedures. These metabolites were further tested for their antimicrobial potential against two-gram positive (Bacillus subtilis and Staphylococcus aureus) and two-gram negative (Escherichia coli and Pasteurella multocida) bacteria and three pathogenic fungal strains (Asprrgillus niger, Fusarium solani and Aspergillus flavus). GC-MS analysis revealed the presence of major constituents like Ca- Caryophyllene (22.70%), α cubebene (11.2%) and alpha Humulene (5.91%). The ethyl-acetate, methanol, n-hexane and chloroform extracts were tested for antibacterial and antifungal activities against above mentioned microbes. Among all the tested solvent extracts, Chloroform and ethyl acetate extracts of P. guajava demonstrated more sensitivity towards the growth of B. subtilis and P. multocida with MIC of 230±3.02, 316. ±6.2 and 237±5.09 and 288±1.55 μg/ml, respectively. Methanolic extracts showed higher MIC against S. aureus (233±5.51 μg/ml) and E. coli (192±2.05 μg/ml), respectively. The findings of this current study would provide the way to use guava as a potential therapeutic agent to combat antimicrobial and antifungal resistance. |
| first_indexed | 2024-03-06T03:22:06Z |
| format | Article |
| id | ums.eprints-34842 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:22:06Z |
| publishDate | 2019 |
| publisher | Pakistan Journal of Agricultural Sciences |
| record_format | dspace |
| spelling | ums.eprints-348422022-11-15T01:34:48Z https://eprints.ums.edu.my/id/eprint/34842/ Study of Gc-ms And Hplc Characterized Metabolic Compounds in Guava (Psidium Guajava L.) Leaves Muhammad Afzal Riffat Iqbal Zahed Mahmood Basit Zeshan Javed Iqbal Wattoo QK1-989 Botany SB1-1110 Plant culture Psidium guajava leaves are rich source of nutrients, antioxidants, phytoconstituents and biological active compounds. The study was designed to elucidate secondary metabolites like alkaloids, saponins, flavonoids, tannins and glycosides in extracts of guava leaves through Gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) by qualitative as well as quantitative procedures. These metabolites were further tested for their antimicrobial potential against two-gram positive (Bacillus subtilis and Staphylococcus aureus) and two-gram negative (Escherichia coli and Pasteurella multocida) bacteria and three pathogenic fungal strains (Asprrgillus niger, Fusarium solani and Aspergillus flavus). GC-MS analysis revealed the presence of major constituents like Ca- Caryophyllene (22.70%), α cubebene (11.2%) and alpha Humulene (5.91%). The ethyl-acetate, methanol, n-hexane and chloroform extracts were tested for antibacterial and antifungal activities against above mentioned microbes. Among all the tested solvent extracts, Chloroform and ethyl acetate extracts of P. guajava demonstrated more sensitivity towards the growth of B. subtilis and P. multocida with MIC of 230±3.02, 316. ±6.2 and 237±5.09 and 288±1.55 μg/ml, respectively. Methanolic extracts showed higher MIC against S. aureus (233±5.51 μg/ml) and E. coli (192±2.05 μg/ml), respectively. The findings of this current study would provide the way to use guava as a potential therapeutic agent to combat antimicrobial and antifungal resistance. Pakistan Journal of Agricultural Sciences 2019 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34842/1/Abstract.pdf text en https://eprints.ums.edu.my/id/eprint/34842/2/Full%20text.pdf Muhammad Afzal and Riffat Iqbal and Zahed Mahmood and Basit Zeshan and Javed Iqbal Wattoo (2019) Study of Gc-ms And Hplc Characterized Metabolic Compounds in Guava (Psidium Guajava L.) Leaves. Pakistan Journal of Agricultural Sciences, 56. pp. 709-713. ISSN 0552-9034 (P-ISSN) , 2076-0906 (E-ISSN) https://pakjas.com.pk/papers/3012.pdf https://doi.org/10.21162/PAKJAS/19.7131 https://doi.org/10.21162/PAKJAS/19.7131 |
| spellingShingle | QK1-989 Botany SB1-1110 Plant culture Muhammad Afzal Riffat Iqbal Zahed Mahmood Basit Zeshan Javed Iqbal Wattoo Study of Gc-ms And Hplc Characterized Metabolic Compounds in Guava (Psidium Guajava L.) Leaves |
| title | Study of Gc-ms And Hplc Characterized Metabolic Compounds in Guava (Psidium Guajava L.) Leaves |
| title_full | Study of Gc-ms And Hplc Characterized Metabolic Compounds in Guava (Psidium Guajava L.) Leaves |
| title_fullStr | Study of Gc-ms And Hplc Characterized Metabolic Compounds in Guava (Psidium Guajava L.) Leaves |
| title_full_unstemmed | Study of Gc-ms And Hplc Characterized Metabolic Compounds in Guava (Psidium Guajava L.) Leaves |
| title_short | Study of Gc-ms And Hplc Characterized Metabolic Compounds in Guava (Psidium Guajava L.) Leaves |
| title_sort | study of gc ms and hplc characterized metabolic compounds in guava psidium guajava l leaves |
| topic | QK1-989 Botany SB1-1110 Plant culture |
| url | https://eprints.ums.edu.my/id/eprint/34842/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34842/2/Full%20text.pdf |
| work_keys_str_mv | AT muhammadafzal studyofgcmsandhplccharacterizedmetaboliccompoundsinguavapsidiumguajavalleaves AT riffatiqbal studyofgcmsandhplccharacterizedmetaboliccompoundsinguavapsidiumguajavalleaves AT zahedmahmood studyofgcmsandhplccharacterizedmetaboliccompoundsinguavapsidiumguajavalleaves AT basitzeshan studyofgcmsandhplccharacterizedmetaboliccompoundsinguavapsidiumguajavalleaves AT javediqbalwattoo studyofgcmsandhplccharacterizedmetaboliccompoundsinguavapsidiumguajavalleaves |