Assessment of genetic diversity of brinjal (Solanum melongena L.) germplasm by RAPD markers
Assessment of genetic diversity in a crop species is prerequisite to its improvement. The use of germplasm with distinct DNA profiles helps to generate genetically diversified breeding populations. The present study was carried out to investigate the genetic diversity in brinjal or eggplant (Solanum...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Gaurav Society of Agricultural Research Information Centre
2014
|
| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/34919/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34919/2/Full%20text.pdf |
| _version_ | 1796911685030641664 |
|---|---|
| author | Z. Islam Mahbuba Siddiqua Md Mehedi Hasan M. A. Nahar Md. Amirul Alam s.M. Shamsuzzaman Mohd Monjurul Alam Mondal Adam Puteh |
| author_facet | Z. Islam Mahbuba Siddiqua Md Mehedi Hasan M. A. Nahar Md. Amirul Alam s.M. Shamsuzzaman Mohd Monjurul Alam Mondal Adam Puteh |
| author_sort | Z. Islam |
| collection | UMS |
| description | Assessment of genetic diversity in a crop species is prerequisite to its improvement. The use of germplasm with distinct DNA profiles helps to generate genetically diversified breeding populations. The present study was carried out to investigate the genetic diversity in brinjal or eggplant (Solanum melongena L.) using random amplified polymorphic DNA (RAPD). Fifteen brinjal germplasm and three decamer primers were used for random polymorphic DNA assay. A total of 17 fragments were obtained, out of which 12 (70.59%) were polymorphic. Each primer generated 4 to 8 amplified fragments with an average of 5.67 fragments per primer. The highest genetic distance (0.8873) and the lowest genetic identity (0.4118) were observed in Laffa (Elongated) versus Jessore L and Dharola combinations. The lowest genetic distance (0.1525) was observed in several cultivars. The unweighted pair-group method of arithmetic means (UPGMA) dendrogram was constructed from genetic distance and all brinjal cultivars were grouped into five clusters. The genetic diversity of brinjal cultivars reported in this study will be useful when planning future crosses amongst these cultivars. |
| first_indexed | 2024-03-06T03:22:20Z |
| format | Article |
| id | ums.eprints-34919 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:22:20Z |
| publishDate | 2014 |
| publisher | Gaurav Society of Agricultural Research Information Centre |
| record_format | dspace |
| spelling | ums.eprints-349192022-11-22T03:24:59Z https://eprints.ums.edu.my/id/eprint/34919/ Assessment of genetic diversity of brinjal (Solanum melongena L.) germplasm by RAPD markers Z. Islam Mahbuba Siddiqua Md Mehedi Hasan M. A. Nahar Md. Amirul Alam s.M. Shamsuzzaman Mohd Monjurul Alam Mondal Adam Puteh QK1-989 Botany SB1-1110 Plant culture Assessment of genetic diversity in a crop species is prerequisite to its improvement. The use of germplasm with distinct DNA profiles helps to generate genetically diversified breeding populations. The present study was carried out to investigate the genetic diversity in brinjal or eggplant (Solanum melongena L.) using random amplified polymorphic DNA (RAPD). Fifteen brinjal germplasm and three decamer primers were used for random polymorphic DNA assay. A total of 17 fragments were obtained, out of which 12 (70.59%) were polymorphic. Each primer generated 4 to 8 amplified fragments with an average of 5.67 fragments per primer. The highest genetic distance (0.8873) and the lowest genetic identity (0.4118) were observed in Laffa (Elongated) versus Jessore L and Dharola combinations. The lowest genetic distance (0.1525) was observed in several cultivars. The unweighted pair-group method of arithmetic means (UPGMA) dendrogram was constructed from genetic distance and all brinjal cultivars were grouped into five clusters. The genetic diversity of brinjal cultivars reported in this study will be useful when planning future crosses amongst these cultivars. Gaurav Society of Agricultural Research Information Centre 2014 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34919/1/Abstract.pdf text en https://eprints.ums.edu.my/id/eprint/34919/2/Full%20text.pdf Z. Islam and Mahbuba Siddiqua and Md Mehedi Hasan and M. A. Nahar and Md. Amirul Alam and s.M. Shamsuzzaman and Mohd Monjurul Alam Mondal and Adam Puteh (2014) Assessment of genetic diversity of brinjal (Solanum melongena L.) germplasm by RAPD markers. Research on Crops, 15. pp. 416-422. ISSN 0972-3226 (P-ISSN) , 2348-7542 (E-ISSN) https://www.indianjournals.com/ijor.aspx?target=ijor:rcr&volume=15&issue=2&article=019 http://dx.doi.org/10.5958/2348-7542.2014.00131.4 http://dx.doi.org/10.5958/2348-7542.2014.00131.4 |
| spellingShingle | QK1-989 Botany SB1-1110 Plant culture Z. Islam Mahbuba Siddiqua Md Mehedi Hasan M. A. Nahar Md. Amirul Alam s.M. Shamsuzzaman Mohd Monjurul Alam Mondal Adam Puteh Assessment of genetic diversity of brinjal (Solanum melongena L.) germplasm by RAPD markers |
| title | Assessment of genetic diversity of brinjal (Solanum melongena L.) germplasm by RAPD markers |
| title_full | Assessment of genetic diversity of brinjal (Solanum melongena L.) germplasm by RAPD markers |
| title_fullStr | Assessment of genetic diversity of brinjal (Solanum melongena L.) germplasm by RAPD markers |
| title_full_unstemmed | Assessment of genetic diversity of brinjal (Solanum melongena L.) germplasm by RAPD markers |
| title_short | Assessment of genetic diversity of brinjal (Solanum melongena L.) germplasm by RAPD markers |
| title_sort | assessment of genetic diversity of brinjal solanum melongena l germplasm by rapd markers |
| topic | QK1-989 Botany SB1-1110 Plant culture |
| url | https://eprints.ums.edu.my/id/eprint/34919/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34919/2/Full%20text.pdf |
| work_keys_str_mv | AT zislam assessmentofgeneticdiversityofbrinjalsolanummelongenalgermplasmbyrapdmarkers AT mahbubasiddiqua assessmentofgeneticdiversityofbrinjalsolanummelongenalgermplasmbyrapdmarkers AT mdmehedihasan assessmentofgeneticdiversityofbrinjalsolanummelongenalgermplasmbyrapdmarkers AT manahar assessmentofgeneticdiversityofbrinjalsolanummelongenalgermplasmbyrapdmarkers AT mdamirulalam assessmentofgeneticdiversityofbrinjalsolanummelongenalgermplasmbyrapdmarkers AT smshamsuzzaman assessmentofgeneticdiversityofbrinjalsolanummelongenalgermplasmbyrapdmarkers AT mohdmonjurulalammondal assessmentofgeneticdiversityofbrinjalsolanummelongenalgermplasmbyrapdmarkers AT adamputeh assessmentofgeneticdiversityofbrinjalsolanummelongenalgermplasmbyrapdmarkers |