In vitro regeneration of popular tobacco varieties of Bangladesh from leaf disc
Regeneration ability of five Nicotiana varieties viz., Virginia, Jati, Motihari, CC Bengal and Sumatra were investigated via callus induction using leaf discs. Explants were cultured on MS medium supplemented with different concentrations and combinations of plant growth regulators. Callus formation...
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| Format: | Article |
| Language: | English English |
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Bangladesh Journals Online (BanglaJOL)
2010
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| Online Access: | https://eprints.ums.edu.my/id/eprint/34932/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34932/2/Full%20text.pdf |
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| author | MA Rahman Md. Amirul Alam MR Hossain A Hossain R Afroz |
| author_facet | MA Rahman Md. Amirul Alam MR Hossain A Hossain R Afroz |
| author_sort | MA Rahman |
| collection | UMS |
| description | Regeneration ability of five Nicotiana varieties viz., Virginia, Jati, Motihari, CC Bengal and Sumatra were investigated via callus induction using leaf discs. Explants were cultured on MS medium supplemented with different concentrations and combinations of plant growth regulators. Callus formation frequency was 67.20%. Among the varieties used, Motihari induced the highest percentage (97.50%) of callus followed by Jati (92.50%) in 2.0 rng/L Kinetin and 2.0 mg/L IAA. Shoots were induced from calli cultured on the same medium. Maximum shoot formation from leaf discs was 82.50% on medium supplemented with 2.0 mg/L Kinetin and 2.0 mg/L IAA. It was also revealed from this study that Motihari was the best variety for callus formation and subsequent plantlet regeneration which is a pre-requisite for vector mediated transformation for varietal improvement of Nicotiana species. The rooting response of regenerated shoots was observed by using 1 /2 MS medium with IBA (0.0, 0.5, and 1.0 mg/L). The highest root formation was found in Motihari (90%) with ½ MS medium supplemented with 0.5 mg/L IBA. After that regenerated plantlets with plenty of roots were transferred successfully to pots and subsequently to the field. |
| first_indexed | 2024-03-06T03:22:22Z |
| format | Article |
| id | ums.eprints-34932 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:22:22Z |
| publishDate | 2010 |
| publisher | Bangladesh Journals Online (BanglaJOL) |
| record_format | dspace |
| spelling | ums.eprints-349322022-11-22T03:37:39Z https://eprints.ums.edu.my/id/eprint/34932/ In vitro regeneration of popular tobacco varieties of Bangladesh from leaf disc MA Rahman Md. Amirul Alam MR Hossain A Hossain R Afroz QK1-989 Botany SB1-1110 Plant culture Regeneration ability of five Nicotiana varieties viz., Virginia, Jati, Motihari, CC Bengal and Sumatra were investigated via callus induction using leaf discs. Explants were cultured on MS medium supplemented with different concentrations and combinations of plant growth regulators. Callus formation frequency was 67.20%. Among the varieties used, Motihari induced the highest percentage (97.50%) of callus followed by Jati (92.50%) in 2.0 rng/L Kinetin and 2.0 mg/L IAA. Shoots were induced from calli cultured on the same medium. Maximum shoot formation from leaf discs was 82.50% on medium supplemented with 2.0 mg/L Kinetin and 2.0 mg/L IAA. It was also revealed from this study that Motihari was the best variety for callus formation and subsequent plantlet regeneration which is a pre-requisite for vector mediated transformation for varietal improvement of Nicotiana species. The rooting response of regenerated shoots was observed by using 1 /2 MS medium with IBA (0.0, 0.5, and 1.0 mg/L). The highest root formation was found in Motihari (90%) with ½ MS medium supplemented with 0.5 mg/L IBA. After that regenerated plantlets with plenty of roots were transferred successfully to pots and subsequently to the field. Bangladesh Journals Online (BanglaJOL) 2010 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34932/1/Abstract.pdf text en https://eprints.ums.edu.my/id/eprint/34932/2/Full%20text.pdf MA Rahman and Md. Amirul Alam and MR Hossain and A Hossain and R Afroz (2010) In vitro regeneration of popular tobacco varieties of Bangladesh from leaf disc. Bangladesh Journal of Agricultural Research, 35. pp. 125-134. ISSN 0258-7122 (P-ISSN) , 2408-8293 (E-ISSN) https://www.banglajol.info/index.php/BJAR/article/view/5873 https://doi.org/10.3329/bjar.v35i1.5873 https://doi.org/10.3329/bjar.v35i1.5873 |
| spellingShingle | QK1-989 Botany SB1-1110 Plant culture MA Rahman Md. Amirul Alam MR Hossain A Hossain R Afroz In vitro regeneration of popular tobacco varieties of Bangladesh from leaf disc |
| title | In vitro regeneration of popular tobacco varieties of Bangladesh from leaf disc |
| title_full | In vitro regeneration of popular tobacco varieties of Bangladesh from leaf disc |
| title_fullStr | In vitro regeneration of popular tobacco varieties of Bangladesh from leaf disc |
| title_full_unstemmed | In vitro regeneration of popular tobacco varieties of Bangladesh from leaf disc |
| title_short | In vitro regeneration of popular tobacco varieties of Bangladesh from leaf disc |
| title_sort | in vitro regeneration of popular tobacco varieties of bangladesh from leaf disc |
| topic | QK1-989 Botany SB1-1110 Plant culture |
| url | https://eprints.ums.edu.my/id/eprint/34932/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34932/2/Full%20text.pdf |
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