Rapid profiling of tropical marine cyanobacterial communities
Cyanobacteria are important organisms in the marine ecosystem as they play vital roles in nutrient cycling and oxygen production. In this study, tropical marine cyanobacterial communities from various sites in Southeast Asia and their associated ecological parameters were examined. 16S rRNA sequence...
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| Format: | Article |
| Language: | English English |
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Elsevier
2019
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| Online Access: | https://eprints.ums.edu.my/id/eprint/34954/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/34954/1/ABSTRACT.pdf |
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| author | Curren, Emily Teruaki Yoshida Victor S. Kuwahara Leong, Sandric Chee Yew |
| author_facet | Curren, Emily Teruaki Yoshida Victor S. Kuwahara Leong, Sandric Chee Yew |
| author_sort | Curren, Emily |
| collection | UMS |
| description | Cyanobacteria are important organisms in the marine ecosystem as they play vital roles in nutrient cycling and oxygen production. In this study, tropical marine cyanobacterial communities from various sites in Southeast Asia and their associated ecological parameters were examined. 16S rRNA sequences of 1502 bp were obtained for barcode amplicon sequencing using the Nanopore MinION™ sequencing platform. Planktonic and benthic cyanobacteria were observed with total of 40 genera and 46 species of cyanobacteria identified from sites in Sabah and Singapore. Species richness analyses showed that the cyanobacterial community from the West Johor Strait was approximately two times greater than that of the East Johor Strait and the Singapore Strait. This is due to the variability in ecological parameters measured between sites such as rainfall, salinity and temperature. This is the first study that has employed this technology for phytoplankton samples with species resolution, providing insight to the diversity of cyanobacteria present in tropical marine ecosystems. Furthermore, the portability of this sequencer enables the real-time monitoring of coastal environments for the specific detection and identification of harmful algal bloom species. |
| first_indexed | 2024-03-06T03:22:25Z |
| format | Article |
| id | ums.eprints-34954 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:22:25Z |
| publishDate | 2019 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | ums.eprints-349542022-11-29T23:57:58Z https://eprints.ums.edu.my/id/eprint/34954/ Rapid profiling of tropical marine cyanobacterial communities Curren, Emily Teruaki Yoshida Victor S. Kuwahara Leong, Sandric Chee Yew GC401-881 Oceanography. By region Cyanobacteria are important organisms in the marine ecosystem as they play vital roles in nutrient cycling and oxygen production. In this study, tropical marine cyanobacterial communities from various sites in Southeast Asia and their associated ecological parameters were examined. 16S rRNA sequences of 1502 bp were obtained for barcode amplicon sequencing using the Nanopore MinION™ sequencing platform. Planktonic and benthic cyanobacteria were observed with total of 40 genera and 46 species of cyanobacteria identified from sites in Sabah and Singapore. Species richness analyses showed that the cyanobacterial community from the West Johor Strait was approximately two times greater than that of the East Johor Strait and the Singapore Strait. This is due to the variability in ecological parameters measured between sites such as rainfall, salinity and temperature. This is the first study that has employed this technology for phytoplankton samples with species resolution, providing insight to the diversity of cyanobacteria present in tropical marine ecosystems. Furthermore, the portability of this sequencer enables the real-time monitoring of coastal environments for the specific detection and identification of harmful algal bloom species. Elsevier 2019 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34954/2/FULL%20TEXT.pdf text en https://eprints.ums.edu.my/id/eprint/34954/1/ABSTRACT.pdf Curren, Emily and Teruaki Yoshida and Victor S. Kuwahara and Leong, Sandric Chee Yew (2019) Rapid profiling of tropical marine cyanobacterial communities. Regional Studies in Marine Science, 25 (100485). pp. 1-10. ISSN 2352-4855 https://www.sciencedirect.com/science/article/abs/pii/S2352485518302792 https://doi.org/10.1016/j.rsma.2018.100485 https://doi.org/10.1016/j.rsma.2018.100485 |
| spellingShingle | GC401-881 Oceanography. By region Curren, Emily Teruaki Yoshida Victor S. Kuwahara Leong, Sandric Chee Yew Rapid profiling of tropical marine cyanobacterial communities |
| title | Rapid profiling of tropical marine cyanobacterial communities |
| title_full | Rapid profiling of tropical marine cyanobacterial communities |
| title_fullStr | Rapid profiling of tropical marine cyanobacterial communities |
| title_full_unstemmed | Rapid profiling of tropical marine cyanobacterial communities |
| title_short | Rapid profiling of tropical marine cyanobacterial communities |
| title_sort | rapid profiling of tropical marine cyanobacterial communities |
| topic | GC401-881 Oceanography. By region |
| url | https://eprints.ums.edu.my/id/eprint/34954/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/34954/1/ABSTRACT.pdf |
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