Inhibitory effect of semi-purified extracts obtained from Potato Tree (Solanum erianthum) against protein kinase and phosphatases
Kinase and phosphatase are two types of protein which copiously involved in the signal transduction cascade. Misleading of these signaling processes will cause cancer development and other related diseases in human. Therefore, over expression of these signaling proteins might be decreased by the pre...
| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
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2019
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| Online Access: | https://eprints.ums.edu.my/id/eprint/34993/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34993/2/Full%20text.pdf |
| _version_ | 1796911697642913792 |
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| author | Azlinah Matawali Nurul Ain Ismail Lee, Ping Chin How, Siew Eng Latifah Saiful Yazan Jualang Azlan Gansau |
| author_facet | Azlinah Matawali Nurul Ain Ismail Lee, Ping Chin How, Siew Eng Latifah Saiful Yazan Jualang Azlan Gansau |
| author_sort | Azlinah Matawali |
| collection | UMS |
| description | Kinase and phosphatase are two types of protein which copiously involved in the signal transduction cascade. Misleading of these signaling processes will cause cancer development and other related diseases in human. Therefore, over expression of these signaling proteins might be decreased by the presence of potential inhibitor. In the present study, an attempt was made to verify the potential of Solanum erianthum collected from Sabah, Malaysia against proteins in signal transduction involved in cancer pathway. Leaves of S. erianthum were extracted using methanol. Extracts were tested against protein phosphatase type 1 (PP1), MAPK kinase (MKK1) and MAPK kinase phosphatase (MSG5); which using PAY704-1 and PAY700-4, MKK1P386 and MKK1P386- MSG5 yeast strains, respectively. The results revealed that methanolic extracts of S. erianthum exhibited toxic activities against all assays. Bioassay-guided fractionation of S. erianthum showed positive activities from CHCl3 fraction (CE) against PP1 protein. Chromatographic separation later confirmed column fractions F1 and F2 of S. erianthum as PP1 inhibitor. In- vitro cell growth inhibition assay of this plant sample showed moderate activities against HeLa, CaOV3 and MCF7 cell lines. |
| first_indexed | 2024-03-06T03:22:31Z |
| format | Conference or Workshop Item |
| id | ums.eprints-34993 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:22:31Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ums.eprints-349932022-12-04T23:54:08Z https://eprints.ums.edu.my/id/eprint/34993/ Inhibitory effect of semi-purified extracts obtained from Potato Tree (Solanum erianthum) against protein kinase and phosphatases Azlinah Matawali Nurul Ain Ismail Lee, Ping Chin How, Siew Eng Latifah Saiful Yazan Jualang Azlan Gansau QK1-989 Botany Kinase and phosphatase are two types of protein which copiously involved in the signal transduction cascade. Misleading of these signaling processes will cause cancer development and other related diseases in human. Therefore, over expression of these signaling proteins might be decreased by the presence of potential inhibitor. In the present study, an attempt was made to verify the potential of Solanum erianthum collected from Sabah, Malaysia against proteins in signal transduction involved in cancer pathway. Leaves of S. erianthum were extracted using methanol. Extracts were tested against protein phosphatase type 1 (PP1), MAPK kinase (MKK1) and MAPK kinase phosphatase (MSG5); which using PAY704-1 and PAY700-4, MKK1P386 and MKK1P386- MSG5 yeast strains, respectively. The results revealed that methanolic extracts of S. erianthum exhibited toxic activities against all assays. Bioassay-guided fractionation of S. erianthum showed positive activities from CHCl3 fraction (CE) against PP1 protein. Chromatographic separation later confirmed column fractions F1 and F2 of S. erianthum as PP1 inhibitor. In- vitro cell growth inhibition assay of this plant sample showed moderate activities against HeLa, CaOV3 and MCF7 cell lines. 2019 Conference or Workshop Item PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34993/1/Abstract.pdf text en https://eprints.ums.edu.my/id/eprint/34993/2/Full%20text.pdf Azlinah Matawali and Nurul Ain Ismail and Lee, Ping Chin and How, Siew Eng and Latifah Saiful Yazan and Jualang Azlan Gansau (2019) Inhibitory effect of semi-purified extracts obtained from Potato Tree (Solanum erianthum) against protein kinase and phosphatases. In: 12th Seminar on Science and Technology, 2 - 3 October 2018, Kota Kinabalu, Sabah, Malaysia. https://iopscience.iop.org/article/10.1088/1742-6596/1358/1/012021 |
| spellingShingle | QK1-989 Botany Azlinah Matawali Nurul Ain Ismail Lee, Ping Chin How, Siew Eng Latifah Saiful Yazan Jualang Azlan Gansau Inhibitory effect of semi-purified extracts obtained from Potato Tree (Solanum erianthum) against protein kinase and phosphatases |
| title | Inhibitory effect of semi-purified extracts obtained from Potato Tree (Solanum erianthum) against protein kinase and phosphatases |
| title_full | Inhibitory effect of semi-purified extracts obtained from Potato Tree (Solanum erianthum) against protein kinase and phosphatases |
| title_fullStr | Inhibitory effect of semi-purified extracts obtained from Potato Tree (Solanum erianthum) against protein kinase and phosphatases |
| title_full_unstemmed | Inhibitory effect of semi-purified extracts obtained from Potato Tree (Solanum erianthum) against protein kinase and phosphatases |
| title_short | Inhibitory effect of semi-purified extracts obtained from Potato Tree (Solanum erianthum) against protein kinase and phosphatases |
| title_sort | inhibitory effect of semi purified extracts obtained from potato tree solanum erianthum against protein kinase and phosphatases |
| topic | QK1-989 Botany |
| url | https://eprints.ums.edu.my/id/eprint/34993/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34993/2/Full%20text.pdf |
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