Biological activities of Chromolena odorata (L.) King and Robinson (Asteraceae) collected from Sabah, Malaysia as protein phosphatase type-1 inhibitor
Chromolena Odorata has been traditionally used as wound healer in local community. The present study investigated the anti-kinase and anti-phosphatases activities on methanolic C. odorata extract. Mutant yeast strains used are MKK1P386, MKK1P386_MSG5, PAY704-1 and PAY700-4. Bioassay guided fractiona...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
The Journal of Phytopharmacology
2019
|
| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/34994/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34994/2/Full%20text.pdf |
| _version_ | 1825715155205357568 |
|---|---|
| author | Azlinah Matawali Lee, Ping Chin Jualang Azlan Gansau |
| author_facet | Azlinah Matawali Lee, Ping Chin Jualang Azlan Gansau |
| author_sort | Azlinah Matawali |
| collection | UMS |
| description | Chromolena Odorata has been traditionally used as wound healer in local community. The present study investigated the anti-kinase and anti-phosphatases activities on methanolic C. odorata extract. Mutant yeast strains used are MKK1P386, MKK1P386_MSG5, PAY704-1 and PAY700-4. Bioassay guided fractionation of C. odorata revealed positive activities of hexane, ethyl acetate and chloroform partitions. Column chromatography of all partitionates later confirmed fraction F2 from chloroform extract had most favorable activity with inhibitory zone ranged between 7±0.0 mm until 15±0.0 mm. Kinetic analysis including maximum enzyme velocity (Vm) and Michealis-Menten constant (Km) were evaluated and compared for both normal and inhibited reactions. Enzyme activity with DiFMUP as substrate showed fraction F2 act as PP1 enzyme inhibitor with the Km value 0.60 mM and Vm value 200 mM/min as compared to the normal enzymatic reaction. Results provided unveil the potential of C. odorata as an effective therapeutic agent. |
| first_indexed | 2024-03-06T03:22:31Z |
| format | Article |
| id | ums.eprints-34994 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:22:31Z |
| publishDate | 2019 |
| publisher | The Journal of Phytopharmacology |
| record_format | dspace |
| spelling | ums.eprints-349942022-12-05T00:07:21Z https://eprints.ums.edu.my/id/eprint/34994/ Biological activities of Chromolena odorata (L.) King and Robinson (Asteraceae) collected from Sabah, Malaysia as protein phosphatase type-1 inhibitor Azlinah Matawali Lee, Ping Chin Jualang Azlan Gansau QK1-989 Botany Chromolena Odorata has been traditionally used as wound healer in local community. The present study investigated the anti-kinase and anti-phosphatases activities on methanolic C. odorata extract. Mutant yeast strains used are MKK1P386, MKK1P386_MSG5, PAY704-1 and PAY700-4. Bioassay guided fractionation of C. odorata revealed positive activities of hexane, ethyl acetate and chloroform partitions. Column chromatography of all partitionates later confirmed fraction F2 from chloroform extract had most favorable activity with inhibitory zone ranged between 7±0.0 mm until 15±0.0 mm. Kinetic analysis including maximum enzyme velocity (Vm) and Michealis-Menten constant (Km) were evaluated and compared for both normal and inhibited reactions. Enzyme activity with DiFMUP as substrate showed fraction F2 act as PP1 enzyme inhibitor with the Km value 0.60 mM and Vm value 200 mM/min as compared to the normal enzymatic reaction. Results provided unveil the potential of C. odorata as an effective therapeutic agent. The Journal of Phytopharmacology 2019 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/34994/1/Abstract.pdf text en https://eprints.ums.edu.my/id/eprint/34994/2/Full%20text.pdf Azlinah Matawali and Lee, Ping Chin and Jualang Azlan Gansau (2019) Biological activities of Chromolena odorata (L.) King and Robinson (Asteraceae) collected from Sabah, Malaysia as protein phosphatase type-1 inhibitor. The Journal of Phytopharmacology, 8. pp. 1-4. ISSN 2320-480X http://www.phytopharmajournal.com/Vol8_Issue1_01.pdf |
| spellingShingle | QK1-989 Botany Azlinah Matawali Lee, Ping Chin Jualang Azlan Gansau Biological activities of Chromolena odorata (L.) King and Robinson (Asteraceae) collected from Sabah, Malaysia as protein phosphatase type-1 inhibitor |
| title | Biological activities of Chromolena odorata (L.) King and Robinson (Asteraceae) collected from Sabah, Malaysia as protein phosphatase type-1 inhibitor |
| title_full | Biological activities of Chromolena odorata (L.) King and Robinson (Asteraceae) collected from Sabah, Malaysia as protein phosphatase type-1 inhibitor |
| title_fullStr | Biological activities of Chromolena odorata (L.) King and Robinson (Asteraceae) collected from Sabah, Malaysia as protein phosphatase type-1 inhibitor |
| title_full_unstemmed | Biological activities of Chromolena odorata (L.) King and Robinson (Asteraceae) collected from Sabah, Malaysia as protein phosphatase type-1 inhibitor |
| title_short | Biological activities of Chromolena odorata (L.) King and Robinson (Asteraceae) collected from Sabah, Malaysia as protein phosphatase type-1 inhibitor |
| title_sort | biological activities of chromolena odorata l king and robinson asteraceae collected from sabah malaysia as protein phosphatase type 1 inhibitor |
| topic | QK1-989 Botany |
| url | https://eprints.ums.edu.my/id/eprint/34994/1/Abstract.pdf https://eprints.ums.edu.my/id/eprint/34994/2/Full%20text.pdf |
| work_keys_str_mv | AT azlinahmatawali biologicalactivitiesofchromolenaodoratalkingandrobinsonasteraceaecollectedfromsabahmalaysiaasproteinphosphatasetype1inhibitor AT leepingchin biologicalactivitiesofchromolenaodoratalkingandrobinsonasteraceaecollectedfromsabahmalaysiaasproteinphosphatasetype1inhibitor AT jualangazlangansau biologicalactivitiesofchromolenaodoratalkingandrobinsonasteraceaecollectedfromsabahmalaysiaasproteinphosphatasetype1inhibitor |