Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities
Herbal products are preferable to synthetic medicines, and the use of traditional medicines is increasing day-by-day. The current study was designed to evaluate the potentials of bioactive compounds from Citrullus colocynthis by performing FTIR, HPLC, and GC-MS analyses, which explore the good conce...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
MDPI
2023
|
| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/35841/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/35841/2/FULL%20TEXT.pdf |
| _version_ | 1796911803518681088 |
|---|---|
| author | Muhammad Afzal Anis Shahzad Khan Basit Zeshan Muhammad Riaz Umer Ejaz Ayesha Saleem Rida Zaineb Haseeb Akram Sindhu Chan, Yean Yean Naveed Ahmed |
| author_facet | Muhammad Afzal Anis Shahzad Khan Basit Zeshan Muhammad Riaz Umer Ejaz Ayesha Saleem Rida Zaineb Haseeb Akram Sindhu Chan, Yean Yean Naveed Ahmed |
| author_sort | Muhammad Afzal |
| collection | UMS |
| description | Herbal products are preferable to synthetic medicines, and the use of traditional medicines is increasing day-by-day. The current study was designed to evaluate the potentials of bioactive compounds from Citrullus colocynthis by performing FTIR, HPLC, and GC-MS analyses, which explore the good concentration of the secondary metabolites, such as gallic acid (74.854 ppm), vanillic acid (122.616 ppm), and ferulic acid (101.045 ppm) with considerable bioactivities. Antimicrobial protein was estimated by performing SDS-PAGE, ranging from 15 to 70 kDa in all protein fractions. The current study also checked the cytotoxicity of the bioactive compounds in the active fraction of C. colocynthis, and to perform this activity, the groups of rats were arranged with 16 rats randomly divided into four groups (three experimental and one control) by administering various dosage of methanolic fractions in dose-dependent manner. Histopathology was conducted on the livers of the rats after 15 days of sacrifice under deep anesthesia. In liver cell slides examined at the maximum dose of 600 mg/kg, minimal morphological changes, such as slight ballooning, nuclear variation, vacuolar degeneration, and hydropic degeneration, were observed. Furthermore, the in silico analysis identified bioactive compounds as potential drug candidates. |
| first_indexed | 2024-03-06T03:24:11Z |
| format | Article |
| id | ums.eprints-35841 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:24:11Z |
| publishDate | 2023 |
| publisher | MDPI |
| record_format | dspace |
| spelling | ums.eprints-358412023-07-12T08:21:00Z https://eprints.ums.edu.my/id/eprint/35841/ Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities Muhammad Afzal Anis Shahzad Khan Basit Zeshan Muhammad Riaz Umer Ejaz Ayesha Saleem Rida Zaineb Haseeb Akram Sindhu Chan, Yean Yean Naveed Ahmed S1-(972) Agriculture (General) SB1-1110 Plant culture Herbal products are preferable to synthetic medicines, and the use of traditional medicines is increasing day-by-day. The current study was designed to evaluate the potentials of bioactive compounds from Citrullus colocynthis by performing FTIR, HPLC, and GC-MS analyses, which explore the good concentration of the secondary metabolites, such as gallic acid (74.854 ppm), vanillic acid (122.616 ppm), and ferulic acid (101.045 ppm) with considerable bioactivities. Antimicrobial protein was estimated by performing SDS-PAGE, ranging from 15 to 70 kDa in all protein fractions. The current study also checked the cytotoxicity of the bioactive compounds in the active fraction of C. colocynthis, and to perform this activity, the groups of rats were arranged with 16 rats randomly divided into four groups (three experimental and one control) by administering various dosage of methanolic fractions in dose-dependent manner. Histopathology was conducted on the livers of the rats after 15 days of sacrifice under deep anesthesia. In liver cell slides examined at the maximum dose of 600 mg/kg, minimal morphological changes, such as slight ballooning, nuclear variation, vacuolar degeneration, and hydropic degeneration, were observed. Furthermore, the in silico analysis identified bioactive compounds as potential drug candidates. MDPI 2023 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/35841/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/35841/2/FULL%20TEXT.pdf Muhammad Afzal and Anis Shahzad Khan and Basit Zeshan and Muhammad Riaz and Umer Ejaz and Ayesha Saleem and Rida Zaineb and Haseeb Akram Sindhu and Chan, Yean Yean and Naveed Ahmed (2023) Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities. Molecules, 28. pp. 1-20. https://doi.org/10.3390/molecules28041743 |
| spellingShingle | S1-(972) Agriculture (General) SB1-1110 Plant culture Muhammad Afzal Anis Shahzad Khan Basit Zeshan Muhammad Riaz Umer Ejaz Ayesha Saleem Rida Zaineb Haseeb Akram Sindhu Chan, Yean Yean Naveed Ahmed Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities |
| title | Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities |
| title_full | Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities |
| title_fullStr | Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities |
| title_full_unstemmed | Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities |
| title_short | Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities |
| title_sort | characterization of bioactive compounds and novel proteins derived from promising source citrullus colocynthis along with in vitro and in vivo activities |
| topic | S1-(972) Agriculture (General) SB1-1110 Plant culture |
| url | https://eprints.ums.edu.my/id/eprint/35841/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/35841/2/FULL%20TEXT.pdf |
| work_keys_str_mv | AT muhammadafzal characterizationofbioactivecompoundsandnovelproteinsderivedfrompromisingsourcecitrulluscolocynthisalongwithinvitroandinvivoactivities AT anisshahzadkhan characterizationofbioactivecompoundsandnovelproteinsderivedfrompromisingsourcecitrulluscolocynthisalongwithinvitroandinvivoactivities AT basitzeshan characterizationofbioactivecompoundsandnovelproteinsderivedfrompromisingsourcecitrulluscolocynthisalongwithinvitroandinvivoactivities AT muhammadriaz characterizationofbioactivecompoundsandnovelproteinsderivedfrompromisingsourcecitrulluscolocynthisalongwithinvitroandinvivoactivities AT umerejaz characterizationofbioactivecompoundsandnovelproteinsderivedfrompromisingsourcecitrulluscolocynthisalongwithinvitroandinvivoactivities AT ayeshasaleem characterizationofbioactivecompoundsandnovelproteinsderivedfrompromisingsourcecitrulluscolocynthisalongwithinvitroandinvivoactivities AT ridazaineb characterizationofbioactivecompoundsandnovelproteinsderivedfrompromisingsourcecitrulluscolocynthisalongwithinvitroandinvivoactivities AT haseebakramsindhu characterizationofbioactivecompoundsandnovelproteinsderivedfrompromisingsourcecitrulluscolocynthisalongwithinvitroandinvivoactivities AT chanyeanyean characterizationofbioactivecompoundsandnovelproteinsderivedfrompromisingsourcecitrulluscolocynthisalongwithinvitroandinvivoactivities AT naveedahmed characterizationofbioactivecompoundsandnovelproteinsderivedfrompromisingsourcecitrulluscolocynthisalongwithinvitroandinvivoactivities |