Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from Lactococcus lactis Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System

Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from Lactococcus lactis Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System

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This work aimed to optimize the parameters affecting partitioning of a bacteriocin-like inhibitory substances (BLIS) from Lactococcus lactis Gh1 in extractive fermentation using polyethylene glycol (PEG)/dextran aqueous two-phase system (ATPS). This system was developed for the simultaneous cell cul...

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Main Authors: Roslina Jawan, Sahar Abbasiliasi, Tan, Joo Shun, Murni Halim, Shuhaimi Mustafa, Lee, Bin Hao, Jia Sim Kwa, Arbakariya B. Ariff
Format: Article
Language:English
English
Published: MDPI 2021
Subjects:
Q1-390 Science (General)
QA1-939 Mathematics
Online Access:https://eprints.ums.edu.my/id/eprint/36168/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/36168/2/FULL%20TEXT.pdf
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author Roslina Jawan
Sahar Abbasiliasi
Tan, Joo Shun
Murni Halim
Shuhaimi Mustafa
Lee, Bin Hao
Jia Sim Kwa
Arbakariya B. Ariff
author_facet Roslina Jawan
Sahar Abbasiliasi
Tan, Joo Shun
Murni Halim
Shuhaimi Mustafa
Lee, Bin Hao
Jia Sim Kwa
Arbakariya B. Ariff
author_sort Roslina Jawan
collection UMS
description This work aimed to optimize the parameters affecting partitioning of a bacteriocin-like inhibitory substances (BLIS) from Lactococcus lactis Gh1 in extractive fermentation using polyethylene glycol (PEG)/dextran aqueous two-phase system (ATPS). This system was developed for the simultaneous cell cultivation and downstream processing of BLIS. Results showed that the molecular weight of PEG, PEG concentration, and dextran T500 affect the partition coefficient (K), purification factor (PF), and yield of BLIS partitioning. ATPS composed of 10% (w/w) PEG2000 and 8% (w/w) dextran T500, provided the greatest conditions for the extractive BLIS production. The K (1.00±0.16), PF (2.92±0.37) and yield (77.24±2.81%) were increased at selected orbital speed (200 rpm) and pH (pH 7). Sustainable growth of the cells in the bioreactor and repeated fermentation up to the eighth extractive batch were observed during the scale up process, ensuring a continuous production and purification of BLIS. Hence, the simplicity and effectiveness of ATPS in the purification of BLIS were proven in this study.
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spelling ums.eprints-361682023-07-24T08:04:20Z https://eprints.ums.edu.my/id/eprint/36168/ Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from Lactococcus lactis Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System Roslina Jawan Sahar Abbasiliasi Tan, Joo Shun Murni Halim Shuhaimi Mustafa Lee, Bin Hao Jia Sim Kwa Arbakariya B. Ariff Q1-390 Science (General) QA1-939 Mathematics This work aimed to optimize the parameters affecting partitioning of a bacteriocin-like inhibitory substances (BLIS) from Lactococcus lactis Gh1 in extractive fermentation using polyethylene glycol (PEG)/dextran aqueous two-phase system (ATPS). This system was developed for the simultaneous cell cultivation and downstream processing of BLIS. Results showed that the molecular weight of PEG, PEG concentration, and dextran T500 affect the partition coefficient (K), purification factor (PF), and yield of BLIS partitioning. ATPS composed of 10% (w/w) PEG2000 and 8% (w/w) dextran T500, provided the greatest conditions for the extractive BLIS production. The K (1.00±0.16), PF (2.92±0.37) and yield (77.24±2.81%) were increased at selected orbital speed (200 rpm) and pH (pH 7). Sustainable growth of the cells in the bioreactor and repeated fermentation up to the eighth extractive batch were observed during the scale up process, ensuring a continuous production and purification of BLIS. Hence, the simplicity and effectiveness of ATPS in the purification of BLIS were proven in this study. MDPI 2021-11 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/36168/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/36168/2/FULL%20TEXT.pdf Roslina Jawan and Sahar Abbasiliasi and Tan, Joo Shun and Murni Halim and Shuhaimi Mustafa and Lee, Bin Hao and Jia Sim Kwa and Arbakariya B. Ariff (2021) Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from Lactococcus lactis Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System. Fermentation, 7. pp. 1-19. https://doi.org/10.3390/fermentation7040257
spellingShingle Q1-390 Science (General)
QA1-939 Mathematics
Roslina Jawan
Sahar Abbasiliasi
Tan, Joo Shun
Murni Halim
Shuhaimi Mustafa
Lee, Bin Hao
Jia Sim Kwa
Arbakariya B. Ariff
Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from Lactococcus lactis Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System
title Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from Lactococcus lactis Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System
title_full Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from Lactococcus lactis Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System
title_fullStr Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from Lactococcus lactis Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System
title_full_unstemmed Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from Lactococcus lactis Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System
title_short Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from Lactococcus lactis Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System
title_sort extractive fermentation for recovery of bacteriocin like inhibitory substances derived from lactococcus lactis gh1 using peg2000 dextran t500 aqueous two phase system
topic Q1-390 Science (General)
QA1-939 Mathematics
url https://eprints.ums.edu.my/id/eprint/36168/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/36168/2/FULL%20TEXT.pdf
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