Revised annotation and characterization of novel Aedes albopictus miRNAs and their potential functions in dengue virus infection
The Asian tiger mosquito, Ae. albopictus, is a highly invasive species that transmits several arboviruses including dengue (DENV), Zika (ZIKV), and chikungunya (CHIKV). Although several studies have identified microRNAs (miRNAs) in Ae. albopictus, it is crucial to extend and improve current annotati...
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| Format: | Article |
| Language: | English English |
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MDPI
2022
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| Online Access: | https://eprints.ums.edu.my/id/eprint/36545/3/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/36545/2/FULLTEXT.pdf |
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| author | Azali Azlan Muhammad Amir Yunus Mardani Abdul Halim Ghows Azzam |
| author_facet | Azali Azlan Muhammad Amir Yunus Mardani Abdul Halim Ghows Azzam |
| author_sort | Azali Azlan |
| collection | UMS |
| description | The Asian tiger mosquito, Ae. albopictus, is a highly invasive species that transmits several arboviruses including dengue (DENV), Zika (ZIKV), and chikungunya (CHIKV). Although several studies have identified microRNAs (miRNAs) in Ae. albopictus, it is crucial to extend and improve current annotations with both the newly improved genome assembly and the increased number of small RNA-sequencing data. We combined our high-depth sequence data and 26 public datasets to re-annotate Ae. albopictus miRNAs and found a total of 72 novel mature miRNAs. We discovered that the expression of novel miRNAs was lower than known miRNAs. Furthermore, compared to known miRNAs, novel miRNAs are prone to expression in a stage-specific manner. Upon DENV infection, a total of 44 novel miRNAs were differentially expressed, and target prediction analysis revealed that miRNA-target genes were involved in lipid metabolism and protein processing in endoplasmic reticulum. Taken together, the miRNA annotation profile provided here is the most comprehensive to date. We believed that this would facilitate future research in understanding virus–host interactions, particularly in the role of miRNAs. |
| first_indexed | 2024-03-06T03:24:54Z |
| format | Article |
| id | ums.eprints-36545 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:24:54Z |
| publishDate | 2022 |
| publisher | MDPI |
| record_format | dspace |
| spelling | ums.eprints-365452023-08-17T04:18:38Z https://eprints.ums.edu.my/id/eprint/36545/ Revised annotation and characterization of novel Aedes albopictus miRNAs and their potential functions in dengue virus infection Azali Azlan Muhammad Amir Yunus Mardani Abdul Halim Ghows Azzam RC109-216 Infectious and parasitic diseases The Asian tiger mosquito, Ae. albopictus, is a highly invasive species that transmits several arboviruses including dengue (DENV), Zika (ZIKV), and chikungunya (CHIKV). Although several studies have identified microRNAs (miRNAs) in Ae. albopictus, it is crucial to extend and improve current annotations with both the newly improved genome assembly and the increased number of small RNA-sequencing data. We combined our high-depth sequence data and 26 public datasets to re-annotate Ae. albopictus miRNAs and found a total of 72 novel mature miRNAs. We discovered that the expression of novel miRNAs was lower than known miRNAs. Furthermore, compared to known miRNAs, novel miRNAs are prone to expression in a stage-specific manner. Upon DENV infection, a total of 44 novel miRNAs were differentially expressed, and target prediction analysis revealed that miRNA-target genes were involved in lipid metabolism and protein processing in endoplasmic reticulum. Taken together, the miRNA annotation profile provided here is the most comprehensive to date. We believed that this would facilitate future research in understanding virus–host interactions, particularly in the role of miRNAs. MDPI 2022-10-19 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/36545/3/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/36545/2/FULLTEXT.pdf Azali Azlan and Muhammad Amir Yunus and Mardani Abdul Halim and Ghows Azzam (2022) Revised annotation and characterization of novel Aedes albopictus miRNAs and their potential functions in dengue virus infection. Biology, 11. pp. 1-12. ISSN 2079-7737 https://doi.org/10.3390/ biology11101536 |
| spellingShingle | RC109-216 Infectious and parasitic diseases Azali Azlan Muhammad Amir Yunus Mardani Abdul Halim Ghows Azzam Revised annotation and characterization of novel Aedes albopictus miRNAs and their potential functions in dengue virus infection |
| title | Revised annotation and characterization of novel Aedes albopictus miRNAs and their potential functions in dengue virus infection |
| title_full | Revised annotation and characterization of novel Aedes albopictus miRNAs and their potential functions in dengue virus infection |
| title_fullStr | Revised annotation and characterization of novel Aedes albopictus miRNAs and their potential functions in dengue virus infection |
| title_full_unstemmed | Revised annotation and characterization of novel Aedes albopictus miRNAs and their potential functions in dengue virus infection |
| title_short | Revised annotation and characterization of novel Aedes albopictus miRNAs and their potential functions in dengue virus infection |
| title_sort | revised annotation and characterization of novel aedes albopictus mirnas and their potential functions in dengue virus infection |
| topic | RC109-216 Infectious and parasitic diseases |
| url | https://eprints.ums.edu.my/id/eprint/36545/3/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/36545/2/FULLTEXT.pdf |
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