LC-MS/MS and GC-MS Analysis for the Identification of Bioactive Metabolites Responsible for the Antioxidant and Antibacterial Activities of Lygodium microphyllum (Cav.) R. Br
Natural products serve as a valuable source of antioxidants with potential health benefits for various conditions. Lygodium microphyllum (Cav.) R. Br., also known as Old World climbing fern, is an invasive climbing fern native to Southeast Asia, Africa, South America, Australia, and Melanesia. It ha...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
MDPI
2023
|
| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/37450/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/37450/2/FULL%20TEXT.pdf |
| _version_ | 1825715361856618496 |
|---|---|
| author | Wuen, Yew Teoh Yoong, Soon Yong Faizan Naeem Razali Sarah Stephenie Muhammad Dawood Shah Jen, Kit Tan Charles Gnanaraj Norhaizan Mohd Esa |
| author_facet | Wuen, Yew Teoh Yoong, Soon Yong Faizan Naeem Razali Sarah Stephenie Muhammad Dawood Shah Jen, Kit Tan Charles Gnanaraj Norhaizan Mohd Esa |
| author_sort | Wuen, Yew Teoh |
| collection | UMS |
| description | Natural products serve as a valuable source of antioxidants with potential health benefits for various conditions. Lygodium microphyllum (Cav.) R. Br., also known as Old World climbing fern, is an invasive climbing fern native to Southeast Asia, Africa, South America, Australia, and Melanesia. It has been reported to possess interesting pharmacological properties including hepatoprotective and anti-inflammatory mechanisms. This study analyzed the potential bioactive metabolites that contribute to the antioxidant and antimicrobial effects of L. microphyllum (LM) by profiling the crude extract using high-resolution LC-MS/MS and GC-MS systems. Several classes of compounds such as phenolics, flavonoids, terpenoids, steroids, macrolides, vitamins, lipids, and other hydrocarbons were found in the crude extract of LM through non-targeted analysis. A total of 74 compounds were detected in LC-MS/MS, whereas a total of nine compounds were identified in GC-MS. Out of the 74 compounds detected in LC-MS/MS, 34 compounds, primarily quercetin, kaempferol, trifolin, pyroglutamic acid, arachidonic acid, and rutin were reported with antioxidant, antimicrobial, antiinflammatory, and hepatoprotective activities. The presence of phenolic and flavonoid compounds with reported bioactivities in the crude extract of LM evidence its pharmacological properties. |
| first_indexed | 2024-03-06T03:25:41Z |
| format | Article |
| id | ums.eprints-37450 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T03:25:41Z |
| publishDate | 2023 |
| publisher | MDPI |
| record_format | dspace |
| spelling | ums.eprints-374502023-10-02T07:23:10Z https://eprints.ums.edu.my/id/eprint/37450/ LC-MS/MS and GC-MS Analysis for the Identification of Bioactive Metabolites Responsible for the Antioxidant and Antibacterial Activities of Lygodium microphyllum (Cav.) R. Br Wuen, Yew Teoh Yoong, Soon Yong Faizan Naeem Razali Sarah Stephenie Muhammad Dawood Shah Jen, Kit Tan Charles Gnanaraj Norhaizan Mohd Esa QK1-474.5 General Including geographical distribution RM300-666 Drugs and their actions Natural products serve as a valuable source of antioxidants with potential health benefits for various conditions. Lygodium microphyllum (Cav.) R. Br., also known as Old World climbing fern, is an invasive climbing fern native to Southeast Asia, Africa, South America, Australia, and Melanesia. It has been reported to possess interesting pharmacological properties including hepatoprotective and anti-inflammatory mechanisms. This study analyzed the potential bioactive metabolites that contribute to the antioxidant and antimicrobial effects of L. microphyllum (LM) by profiling the crude extract using high-resolution LC-MS/MS and GC-MS systems. Several classes of compounds such as phenolics, flavonoids, terpenoids, steroids, macrolides, vitamins, lipids, and other hydrocarbons were found in the crude extract of LM through non-targeted analysis. A total of 74 compounds were detected in LC-MS/MS, whereas a total of nine compounds were identified in GC-MS. Out of the 74 compounds detected in LC-MS/MS, 34 compounds, primarily quercetin, kaempferol, trifolin, pyroglutamic acid, arachidonic acid, and rutin were reported with antioxidant, antimicrobial, antiinflammatory, and hepatoprotective activities. The presence of phenolic and flavonoid compounds with reported bioactivities in the crude extract of LM evidence its pharmacological properties. MDPI 2023 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/37450/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/37450/2/FULL%20TEXT.pdf Wuen, Yew Teoh and Yoong, Soon Yong and Faizan Naeem Razali and Sarah Stephenie and Muhammad Dawood Shah and Jen, Kit Tan and Charles Gnanaraj and Norhaizan Mohd Esa (2023) LC-MS/MS and GC-MS Analysis for the Identification of Bioactive Metabolites Responsible for the Antioxidant and Antibacterial Activities of Lygodium microphyllum (Cav.) R. Br. Separations, 10. pp. 2-12. https://doi.org/10.3390/separations10030215 |
| spellingShingle | QK1-474.5 General Including geographical distribution RM300-666 Drugs and their actions Wuen, Yew Teoh Yoong, Soon Yong Faizan Naeem Razali Sarah Stephenie Muhammad Dawood Shah Jen, Kit Tan Charles Gnanaraj Norhaizan Mohd Esa LC-MS/MS and GC-MS Analysis for the Identification of Bioactive Metabolites Responsible for the Antioxidant and Antibacterial Activities of Lygodium microphyllum (Cav.) R. Br |
| title | LC-MS/MS and GC-MS Analysis for the Identification of Bioactive Metabolites Responsible for the Antioxidant and Antibacterial Activities of Lygodium microphyllum (Cav.) R. Br |
| title_full | LC-MS/MS and GC-MS Analysis for the Identification of Bioactive Metabolites Responsible for the Antioxidant and Antibacterial Activities of Lygodium microphyllum (Cav.) R. Br |
| title_fullStr | LC-MS/MS and GC-MS Analysis for the Identification of Bioactive Metabolites Responsible for the Antioxidant and Antibacterial Activities of Lygodium microphyllum (Cav.) R. Br |
| title_full_unstemmed | LC-MS/MS and GC-MS Analysis for the Identification of Bioactive Metabolites Responsible for the Antioxidant and Antibacterial Activities of Lygodium microphyllum (Cav.) R. Br |
| title_short | LC-MS/MS and GC-MS Analysis for the Identification of Bioactive Metabolites Responsible for the Antioxidant and Antibacterial Activities of Lygodium microphyllum (Cav.) R. Br |
| title_sort | lc ms ms and gc ms analysis for the identification of bioactive metabolites responsible for the antioxidant and antibacterial activities of lygodium microphyllum cav r br |
| topic | QK1-474.5 General Including geographical distribution RM300-666 Drugs and their actions |
| url | https://eprints.ums.edu.my/id/eprint/37450/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/37450/2/FULL%20TEXT.pdf |
| work_keys_str_mv | AT wuenyewteoh lcmsmsandgcmsanalysisfortheidentificationofbioactivemetabolitesresponsiblefortheantioxidantandantibacterialactivitiesoflygodiummicrophyllumcavrbr AT yoongsoonyong lcmsmsandgcmsanalysisfortheidentificationofbioactivemetabolitesresponsiblefortheantioxidantandantibacterialactivitiesoflygodiummicrophyllumcavrbr AT faizannaeemrazali lcmsmsandgcmsanalysisfortheidentificationofbioactivemetabolitesresponsiblefortheantioxidantandantibacterialactivitiesoflygodiummicrophyllumcavrbr AT sarahstephenie lcmsmsandgcmsanalysisfortheidentificationofbioactivemetabolitesresponsiblefortheantioxidantandantibacterialactivitiesoflygodiummicrophyllumcavrbr AT muhammaddawoodshah lcmsmsandgcmsanalysisfortheidentificationofbioactivemetabolitesresponsiblefortheantioxidantandantibacterialactivitiesoflygodiummicrophyllumcavrbr AT jenkittan lcmsmsandgcmsanalysisfortheidentificationofbioactivemetabolitesresponsiblefortheantioxidantandantibacterialactivitiesoflygodiummicrophyllumcavrbr AT charlesgnanaraj lcmsmsandgcmsanalysisfortheidentificationofbioactivemetabolitesresponsiblefortheantioxidantandantibacterialactivitiesoflygodiummicrophyllumcavrbr AT norhaizanmohdesa lcmsmsandgcmsanalysisfortheidentificationofbioactivemetabolitesresponsiblefortheantioxidantandantibacterialactivitiesoflygodiummicrophyllumcavrbr |